Simultaneous Detection of Adenosine-to-Inosine Editing and -Methyladenosine at Identical RNA Sites through Deamination-Assisted Reverse Transcription Stalling.

Adenosine-to-inosine (A-to-I) editing and -methyladenosine (mA) modifications are pivotal RNA modifications with widespread functional significance in physiological and pathological processes. Although significant effort has been dedicated to developing methodologies for identifying and quantifying these modifications, traditional approaches have often focused on each modification independently, neglecting the potential co-occurrence of A-to-I editing and mA modifications at the same adenosine residues. This limitation has constrained our understanding of the intricate regulatory mechanisms governing RNA function and the interplay between different types of RNA modifications.

Single-Nucleotide Resolution Mapping of -Methyladenine in Genomic DNA.

-Methyladenine (6mA) is a naturally occurring DNA modification in both prokaryotes and eukaryotes. Herein, we developed a deaminase-mediated sequencing (DM-seq) method for genome-wide mapping of 6mA at single-nucleotide resolution. The method capitalizes on the selective deamination of adenine, but not 6mA, in DNA mediated by an evolved adenine deaminase, ABE8e.

Single-Base Resolution Detection of -Methyladenosine in RNA by Adenosine Deamination Sequencing.

-Methyladenosine (mA) is one of the most abundant and prevalent natural modifications occurring in diverse RNA species. mA plays a wide range of roles in physiological and pathological processes. Revealing the functions of mA relies on the faithful detection of individual mA sites in RNA.

Occurrence and migration of phthalates in adhesive materials to fruits and vegetables.

Labels or tapes are widely used on fresh fruits and vegetables, which may contain phthalates (PAEs). There are few studies on the contamination pathway of PAEs from labels or tapes to food stuff. In this study, the concentrations of eleven PAEs in adhesive labels, tapes, labeled fruits and vegetables on the market were investigated.

Burden and Risk of Polychlorinated Naphthalenes in Chinese Human Milk and a Global Comparison of Human Exposure.

Polychlorinated naphthalenes (PCNs) are carcinogenic contaminants. Residues from historical production and ongoing unintentional releases from industrial thermal sources have led to the ubiquitous presence of PCNs in the environment. Our previous study has revealed that unintentional releases may be the main sources of PCNs in human milk from China.

Polychlorinated naphthalenes in human milk: Health risk assessment to nursing infants and source analysis.

Polychlorinated naphthalenes are teratogenic environmental contaminants. Mother milk is the most important food for nursing infants. The World Health Organization actively promotes breastfeeding for its immunological, psychological, and economic advantages.