Discovery of a heparan sulfate binding domain in monkeypox virus H3 as an anti-poxviral drug target combining AI and MD simulations.

Viral adhesion to host cells is a critical step in infection for many viruses, including monkeypox virus (MPXV). In MPXV, the H3 protein mediates viral adhesion through its interaction with heparan sulfate (HS), yet the structural details of this interaction have remained elusive. Using AI-based structural prediction tools and molecular dynamics (MD) simulations, we identified a novel, positively charged α-helical domain in H3 that is essential for HS binding.

Dissecting Interactions of and to Shape Kiwifruit Wine Flavor.

Mixed fermentation with and has been shown to enhance wine aroma, yet the underlying mechanisms remain unclear. Monoculture of , monoculture of , and mixed culture of and were conducted, and the study analyzed and compared the biomass, flavor profile, and transcriptome responses of the three groups. Both yeast species exhibited growth inhibition in mixed culture, especially .

Innovation in manufacturing SMEs during the COVID-19 pandemic: How does environmental dynamism reinforce employee proactive behavior?

In a turbulent environment such as during the COVID-19 pandemic crisis, employee proactive behavior is imperative for innovation initiatives in small- and medium-sized enterprises (SMEs). We ask whether and how turbulent environments motivate employees to proactively engage in innovative behavior. This study argues that employees' perceptions of environmental dynamism reinforce employee proactive innovation behavior.

Conditionally ablated Pten in prostate basal cells promotes basal-to-luminal differentiation and causes invasive prostate cancer in mice.

Prostate glands comprise two major epithelial cell types: luminal and basal. Luminal cells have long been considered the cellular origin of prostate cancer (CaP). However, recent evidence from a prostate regeneration assay suggests that prostate basal cells can also give rise to CaP.

Mass spectrometry-based analyses for identifying and characterizing S-nitrosylation of protein tyrosine phosphatases.

All members in the protein tyrosine phosphatase (PTP) family of enzymes contain an invariant Cys residue which is absolutely indispensable for catalysis. Due to the unique microenvironment surrounding the active center of PTPs, this Cys residue exhibits an unusually low pKa characteristic, thus being highly susceptible to oxidation or S-nitrosylation. While oxidation-dependent regulation of PTP activity has been extensively examined, the molecular details and biological consequences of PTP S-nitrosylation remain unexplored.

[Effect of noninvasive positive pressure ventilation on treatment of acute respiratory distress syndrome].

Objective: To investigate the factors affecting the effect of noninvasive positive pressure ventilation (NIPPV) on treatment of acute respiratory distress syndrome (ARDS).

Methods: According to the disease-induced factors, patients with ARDS were divided into two groups: group A (pulmonary factors), group B (extra-pulmonary factors). Different kinds of ventilator s were used in the course of NIPPV via facial or nasal mask.

[Changes of blood vessel active substances in acute pulmonary embolism induced by echinococus granulous cyst in rabbits].

Objective: To investigate the role of blood vessel active substances, such as endothelin-1 (ET-1), thromboxane B(2) (TXB(2)), and 6-keto-prostaglandin F(1alpha) (6-K-PGF(1alpha)) in a rabbit model of acute pulmonary embolism induced by echinococus granulous cyst.

Methods: Catheters were placed into the femoral artery and the femoral vein. Saline, fresh clear cyst fluid or sand containing cyst fluid in a volume of 2 ml/kg were infused through the femoral vein catheter.