Development of a Bioorthogonal Click-to-Release Reaction for Hydrogen Polysulfide (HS) Detection.

In this study, we present an innovative "click-to-release" strategy for the design of highly specific HS bioorthogonal probes that undergo a specific click reaction with HS and release fluorophores by a following rearrangement. A library of cyclooctyne derivatives was established and successfully demonstrated the availability of the release strategy. Then, a model probe was synthesized, which can achieve effective fluorophore release (>80%) in the presence of a HS donor.