Health-related quality of life in Chinese medical staff: a latent profile analysis.

Objective: To investigate subgroups of health-related quality of life (HRQoL) in the Chinese medical staff and identify the demographic factors associated with these profiles.

Methods: 574 Chinese medical staff were surveyed online. HRQoL was measured by using the 36-Item Short Form Health Survey, Version 2.

How to Evaluate Green Development Policy Based on the PMC Index Model: Evidence from China.

Implementing green development is important to realizing a harmonious relationship between humans and nature, and has attracted the attention of governments all over the world. This paper uses the PMC (Policy Modeling Consistency) model to make a quantitative evaluation of 21 representative green development policies issued by the Chinese government. The research finds: firstly, the overall evaluation grade of green development is good and the average PMC index of China's 21 green development policies is 6.

How to Evaluate the Level of Green Development Based on Entropy Weight TOPSIS: Evidence from China.

Evaluating the level of green development is of great significance to better implement the concept of green development. By constructing an evaluation index system for green development, this paper comprehensively uses the entropy weight Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS) method and coefficient of variation method to evaluate the green development level of 30 provinces in China from 2010 to 2019 and analyzes the regional differences of green development in China. The research findings are as follows: First, the level of green development in China is low but shows a slow rise trend, from 2010 to 2019; China's green development level rises from 0.

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect.

With reduced background and high sensitivity, photoelectrochemistry (PEC) may be applied as an intracellular nanotool and open a new technological direction of single-cell study. Nevertheless, the present palette of single-cell tools lacks such a PEC-oriented solution. Here a dual-functional photocathodic single-cell nanotool capable of direct electroosmotic intracellular drug delivery and evaluation of oxidative stress is devised by engineering a target-specific organic molecule/NiO/Ni film at the tip of a nanopipette.

The Attitude of Work-Oriented and Family-Oriented Chinese Women Toward the Evaluations Based on the Traditional Positive Stereotype That Women Are Virtuous.

People typically reject being negatively stereotyped but overlook the ways in which they are positively stereotyped. The current study focused on the attitude of Chinese women toward being evaluated based on the traditional positive stereotype that women are virtuous; family/work centrality as a boundary condition of these attitudes; and three perceptions that may mediate the link between this type of evaluation and attitudes of women. In experiment 1, female college students were identified as work-oriented or family-oriented based on their responses to a questionnaire regarding their focus on these two domains.

A Practical Electrochemical Nanotool for Facile Quantification of Amino Acids in Single Cell.

Though significant advances are made in the arena of single-cell electroanalysis, quantification of intracellular amino acids of human cells remains unsolved. Exemplified by l-histidine (l-His), this issue is addressed by a practical electrochemical nanotool synergizing the highly accessible nanopipette with commercially available synthetic DNAzyme. The fabricated nanotools are screened before operation of a single-use manner, and the l-His-provoked cleavage of the DNA molecules can be sensibly transduced by the ionic current rectification response, the intrinsic property of nanopipette governed by its interior surface charges.

Photocontrolled Nanopipette Biosensor for ATP Gradient Electroanalysis of Single Living Cells.

Emerging nanopipette tools have demonstrated substantial potential for advanced single-cell analysis, which plays vital roles from fundamental cellular biology to biomedical diagnostics. Highly recyclable nanopipettes with easy and quick regeneration are of special interest for precise and multiple measurements. However, existing recycle strategies are generally plagued by operational complexity and limited efficiency.

An untargeted metabolomic insight into the high-pressure stress effect on the germination of wholegrain Oryza sativa L.

High hydrostatic pressure (HHP) technique is used as a novel abiotic stress factor for efficiently enhancing the biosynthesis of selected bioactive phytochemicals in germinated wholegrain, but the information about HHP stress-induced metabolic changes remains rather limited. Thus, the current work employed an untargeted gas chromatography-mass spectrometry-based metabolomic approach combining with multivariate models to analyze the effect of mild HHP stress (30 MPa/5 min) on the overall metabolome shifts of wholegrain brown rice (WBR) during germination. Simultaneously, major phenolics in germinated WBR (GBR) were detected by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, to explore the potential relationship between HHP stress-induced rice metabolome alternations and the biotransformation of bioactive components.

An Integrated Electrochemical Nanodevice for Intracellular RNA Collection and Detection in Single Living Cell.

New tools for single-cell interrogation enable deeper understanding of cellular heterogeneity and associated cellular behaviors and functions. Information of RNA expression in single cell could contribute to our knowledge of the genetic regulatory circuits and molecular mechanism of disease development. Although significant progresses have been made for intracellular RNA analysis, existing methods have a trade-off between operational complexity and practical feasibility.

Target-Triggered Assembly in a Nanopipette for Electrochemical Single-Cell Analysis.

Engineered nanopipette tools have recently emerged as a powerful approach for electrochemical nanosensing, which has major implications in both fundamental biological research and biomedical applications. Herein, we describe a generic method of target-triggered assembly of aptamers in a nanopipette for nanosensing, which is exemplified by sensitive and rapid electrochemical single-cell analysis of adenosine triphosphate (ATP), a ubiquitous energy source in life and important signaling molecules in many physiological processes. Specifically, a layer of thiolated aptamers is immobilized onto a Au-coated interior wall of a nanopipette tip.

Effects of microbial fermentation on the flavor of cured duck legs.

In this study, fermentation with Lactobacillus plantarum and Saccharomyces cerevisiae was applied to improve the flavor of cured duck leg meat. Odor and taste evaluations, lipid oxidation, volatile flavor substances, and protein degradation were determined to investigate the effects of microbial fermentation on flavor improvement. The results showed that the utilization of L.

CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing.

The photoelectrochemical (PEC) biosensor, in which light is utilized to excite the photoactive species and current is employed as the detection signal, is a newly appeared yet dynamically developing technique for biological analysis. Based on the assay of DNA binding proteins upon visible light irradiation, a PEC biosensor is constructed for successfully probing a DNA-protein interaction.

Simple-categorization dominance and evaluation of the crossed-categorization target: comparison of different perspectives.

The current study analyzed the effect of simple-categorization dominance on the evaluation of crossed-categorization target from the perspective of observer (other) and actor (self). With a focus on the young-poor target, in Study 1 and Study 2, participants were assigned to observer or actor perspective and were randomly assigned to one of three experimental groups: age simple-categorization dominant, wealth simple-categorization dominant, and a control group. Study 1 demonstrated that in the observer perspective group, the evaluation of the competence of the young-poor target in the wealth simple-categorization dominant group was higher than that in the age simple-categorization dominant group.

Three-Dimensional TiO@CuO@Nickel Foam Electrodes: Design, Characterization, and Validation of O-Independent Photocathodic Enzymatic Bioanalysis.

This work reports the innovative design and application of a three-dimensional (3D) TiO@CuO@nickel foam electrode synergized with enzyme catalysis toward the proof-of-concept study for oxygen-independent photocathodic enzymatic detection. Specifically, a 3D-nanostructured photoelectrode has great potential in the semiconductor-based photoelectrochemical (PEC) biological analysis. On the other hand, using various photocathodes, cathodic PEC bioanalysis, especially the photocathodic enzymatic detection, represents an attractive frontier in the field.

Revealing transient events of molecular recognition via super-localization imaging of single-particle motion.

Molecular recognition plays an important role in biological systems and relates to a wide range of applications in disease diagnostics and therapeutics. Studies based on steady state or ensemble analysis may mask critical dynamic information of single recognition events. Here we report a study of monitoring the transient molecular recognition via single particle motion.

Ultrasmall Nanopipette: Toward Continuous Monitoring of Redox Metabolism at Subcellular Level.

Ionic current rectification (ICR) based nanopipettes allow accurate monitoring of cellular behavior in single living cells. Herein, we proposed a 30 nm nanopipette functionalized with G-quadruplex DNAzyme as an efficient biomimetic recognizer for ROS generation at subcellular level via the changes of current-voltage relationship. Taking advantages of the ultra-small tip, the nanopipette could penetrate into a single living cell repeatedly or keep measuring for a long time without compromising the cellular functions.

Organic Photo-Electrochemical Transistor-Based Biosensor: A Proof-of-Concept Study toward Highly Sensitive DNA Detection.

Organic bioelectronics have shown promising applications for various sensing purposes due to their significant advantages in term of high flexibility, portability, easy fabrication, and biocompatibility. Here, a new type of organic device, organic photo-electrochemical transistor (OPECT), is reported, which is the combination of an organic electrochemical transistor and a photo-electrochemical gate electrode modified with CdS quantum dots (QDs). Thanks to the inherent amplification function of the transistor, the OPECT-based biosensor exhibits much higher sensitivity than that of a traditional biosensor.

Semiconducting Organic-Inorganic Nanodots Heterojunctions: Platforms for General Photoelectrochemical Bioanalysis Application.

In this study, semiconducting organic polymer dots (Pdots) and inorganic quantum dots (Qdots) were first utilized to construct the organic-inorganic nanodots heterojunction for the photoelectrochemical (PEC) bioanalysis application. Specifically, n-type CdS Qdots, p-type CdTe Qdots, and tetraphenylporphyrin (TPP)-doped poly[(9,9-dioctylfluorenyl-2,7-diyl)- co-(1,4-benzo-{2,1',3}-thiadazole)] (PFBT) Pdots were fabricated, and their energy levels, that is, their valence band (VB)/conduction band (CB) or lowest unoccupied molecular orbital (LUMO)/highest occupied molecular orbital (HOMO) values, were also determined. Then, these nanodots were integrated to construct four types of p-n and p-p organic-inorganic nanodots heterojunctions, that is, CdS Qdots/TPP-doped PFBT Pdots, TPP-doped PFBT Pdots/CdS Qdots, CdTe Qdots/TPP-doped PFBT Pdots, and TPP-doped PFBT Pdots/CdTe Qdots, on the transparent glass electrode.

Bismuth Oxyiodide Couples with Glucose Oxidase: A Special Synergized Dual-Catalysis Mechanism for Photoelectrochemical Enzymatic Bioanalysis.

On the basis of a special synergized dual-catalysis mechanism, this work reports the preparation of a BiOI-based heterojunction and its use for cathodic photoelectrochemical (PEC) oxidase biosensing, which, unexpectedly, revealed that hydrogen peroxide (HO) had a greater impact than dioxygen (O). Specifically, the BiOI layer was in situ formed on the substrate through an impregnating hydroxylation method for the following coupling with the model enzyme of glucose oxidases (GOx). The constructed cathodic PEC enzyme sensor exhibited a good analytical performance of rapid response, high stability, and good selectivity.

Nanochannels Photoelectrochemical Biosensor.

Nanochannels have brought new opportunities for biosensor development. Herein, we present the novel concept of a nanochannels photoelectrochemical (PEC) biosensor based on the integration of a unique CuO-nanopyramid-islands (NPIs) photocathode, an anodic aluminum oxide (AAO) membrane, and alkaline phosphatase (ALP) catalytic chemistry. The CuO-NPIs photocathode possesses good performance, and further assembly with AAO yields a designed architecture composed of vertically aligned, highly ordered nanoarrays on top of the CuO-NPIs film.

Photoelectrochemical Bioanalysis Platform of Gold Nanoparticles Equipped Perovskite BiNbOCl.

We have developed sensitive photoelectrochemical (PEC) detection of cysteine using the gold nanoparticles (Au NPs) equipped perovskite BiNbOCl heterostructure. The BiNbOCl was prepared by a solid-state reaction, and the Au NPs/BiNbOCl electrode was made through the electrostatic layer-by-layer self-assembly technique. The Au NPs/BiNbOCl electrode provided much enhanced photocurrent with a great increase compared to the bare BiNbOCl electrode and allowed for the plasmon-enhanced PEC detection of cysteine with good performance.

Quantum-dots-based photoelectrochemical bioanalysis highlighted with recent examples.

Photoelectrochemical (PEC) bioanalysis is a newly developed methodology that provides an exquisite route for innovative biomolecular detection. Quantum dots (QDs) are semiconductor nanocrystals with unique photophysical properties that have attracted tremendous attentions among the analytical community. QDs-based PEC bioanalysis comprises an important research hotspot in the field of PEC bioanalysis due to its combined advantages and potentials.

Simultaneous photoelectrochemical and visualized immunoassay of β-human chorionic gonadotrophin.

Herein, on the basis of the alkaline phosphate (ALP) induced reaction, a simultaneous photoelectrochemical (PEC) and visualized immunoassay has been established for the detection of β-human chorionic gonadotrophin (β-HCG). Specifically, in the proposed system, ALP stimulated the oxidative hydrolyzing transformation of 5-bromo-4-chloro-3-indoyl phosphate (BCIP) to an indigo precipitation, generating an insulating layer that impeded the interfacial mass and electron transfer and thus the photocurrent production. Meanwhile, a visualized detection could be performed according to the change of color intensity.