A phenothiazine coumarin based ratiometric fluorescent probe for real-time detection of lysosomal hypochlorite in living cell and zebra fish.

Hypochlorite (ClO), a type of reactive oxygen species (ROS), plays an essential role in complex biological systems. Real-time detection of the content and distribution of ClO in cells or subcellular organelle is critically essential. In this paper, a lysosomal-targeted fluorescent probe, Cou-Lyso, was constructed for real-time detection of ClO in a ratiometric manner, achieving high sensitivity with a low detection limit (0.

A bifunctional fluorescent probe for simultaneous detection of GSH and HS (n > 1) from different channels with long-wavelength emission.

In this work, we presented a long-wavelength emission fluorescent probe DCM-Cou-SePh that can discriminatively detect glutathione (GSH) and hydrogen polysulfides (HS, n > 1) from green and red emission channels, respectively. With the addition of GSH, probe DCM-Cou-SePh displayed green fluorescence emission (λ = 430/530 nm). In the presence of HS, the probe exhibited a significant fluorescence enhancement in red channel (λ = 560/680 nm).