Diagnostic potential of rhinovirus C using reverse-transcription loop-mediated isothermal amplification (RT-LAMP).

Rhinoviruses (RVs), particularly RV-C, frequently cause acute respiratory infections and asthma exacerbations. However, there is a lack of routine detection methods. Thus, this study aims to develop a rapid molecular and differential diagnostic detection method for RV-C using the reverse transcription (RT) loop-mediated isothermal amplification (LAMP) approach.

A Multi-landscape Assessment of Leptospira Prevalence on a Diversity of Small Mammals.

Leptospirosis is a major zoonotic disease, especially in the tropics, and rodents were known to be carriers of this bacterium. There was established information on Leptospira prevalence among animal reservoirs in human-dominated landscapes from previous literature. However, there was very little focus given comparing the prevalence of Leptospira in a wide range of habitats.

Room-temperature stable loop-mediated isothermal amplification (LAMP) reagents to detect leptospiral DNA.

Background: Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting spp. However, LAMP is hampered by cold storage to maintain the enzymatic activity of DNA polymerase.

Objective: To overcome the drawback of cold storage requirement for LAMP reagents we modified the reagents by adding sucrose as stabilizer.