RfaH contributes to maximal colonization and full virulence of hypervirulent .

Hypervirulent (hvKp) have emerged as clinically important pathogens, posing a serious threat to human health. RfaH, a transcriptional elongation factor, has been regarded as implicated in facilitating the transcription of long virulence operons in certain bacterial species. In , RfaH plays a vital role in promoting CPS synthesis and hypermucoviscosity, as well as mediating bacterial fitness during lung infection.

Isolation and characterization of three novel lytic phages against K54 serotype carbapenem-resistant hypervirulent .

The emergence of carbapenem-resistant hypervirulent (CR-hvKP) has driven us to explore alternative treatments for the limitation of antimicrobial agents. Lytic phages are considered a promising alternative treatment for CR-hvKP infection. In this study, we reported three novel lytic phages, vB_KpnA_SCNJ1-Z, vB_KpnS_SCNJ1-C, and vB_KpnM_SCNJ1-Y, against a CR-hvKP strain SCNJ1, and they possess genomes of double-stranded DNA with a size of 43,428 bp, 46,039 bp, and 50,360 bp, respectively.

Genomic characterization of tigecycline-resistant and isolates from hospital sewage.

Introduction: The tigecycline-resistant Enterobacterales have emerged as a great public concern, and the mobile (X) variants and efflux pump are mainly responsible for the spread of tigecycline resistance. Hospital sewage is considered as an important reservoir of antimicrobial resistance, while tigecycline resistance in this niche is under-researched.

Methods: In this study, five and six strains were selected from a collection of tigecycline-resistant Enterobacterales for further investigation by antimicrobial susceptibility testing, conjugation, whole-genome sequencing, and bioinformatics analysis.

A new class A beta-lactamase gene bla coexists with bla in a novel untypable plasmid in Comamonas aquatica.

Antimicrobial resistance, especially carbapenem resistance, poses a serious threat to global public health. Here, a carbapenem-resistant Comamonas aquatica isolate SCLZS63 was recovered from hospital sewage. Whole-genome sequencing showed that SCLZS63 has a 4,048,791-bp circular chromosome and three plasmids.

Genomic characteristics of clinical multidrug-resistant isolates from a tertiary care hospital in southwest China.

Multidrug-resistant (MDR) , especially those strains producing extended-spectrum β-lactamases (ESBL) and carbapenemases, represents a major public health concern. In the present work, we characterized 27 MDR clinical isolates, including 23 , three , and one , by whole-genome analysis. Among the 27 isolates analyzed, SXT/R391 ICEs were detected in 14 strains, and the complete sequences of nine ICEs were obtained.

Genomic characterization of a carbapenem-resistant Citrobacter freundii cocarrying bla and bla.

Objectives: Citrobacter freundii is an important opportunistic pathogen, and carbapenem-resistant strains pose a significant challenge to public health. Here we report the genetic features of antimicrobial resistance genes of a carbapenem-resistant C. freundii SCLZS47 from hospital sewage by using whole genome sequencing.

Genetic and virulence characteristics of a Raoultella planticola isolate resistant to carbapenem and tigecycline.

Raoultella planticola is an emerging pathogen causing several infections in humans, and its roles in the propagation of antibiotic resistance genes (ARGs) remain uncharacterized. In this study, a carbapenem and tigecycline-resistant R. planticola isolate was recovered from hospital sewage.

Genomic Characterization of a sp. Strain of Animal Origin Co-Carrying and .

The emergence of carbapenem-resistant represents a serious threat to global public health due to limited antibiotic treatment options. Here, we characterize a isolate NMG38-2 of swine origin that exhibits extensive drug resistance, including carbapenems. Whole-genome sequencing based on Illumina and MinION platforms showed that NMG38-2 contains 24 acquired antibiotic resistance genes and three plasmids, among which, pNDM_NMG38-2, a pPvSC3-like plasmid, is transferable and co-carries and .

Whole-Genomic Analysis of NDM-5-Producing Enterobacteriaceae Recovered from an Urban River in China.

Purpose: Three NDM-5-producing Enterobacteriaceae (, and , one each) were isolated during a screening study for the presence of carbapenemase-producing Enterobacteriaceae (CPE) strains in urban rivers in China. The aim of the present study was to characterize these NDM-5-producing isolates by using whole-genome analysis.

Methods: In vitro susceptibility testing was performed using the broth microdilution method.