Simultaneous Dual-Site Identification of 5C/8G in DNA Triplex Using a Nanopore Sensor.

DNA triplex participates in delivering site-specific epigenetic modifications critical for the regulation of gene expression. Among these marks, 5C with 8G functions comprehensively on gene expression. Recently, few research studies have emphasized the necessity of incorporation detection of 5C with 8G using one DNA triplex at the same time.

De novo profiling of insect-resistant proteins of rice via nanopore peptide differentiation.

Nanopore is used as a single-molecule detector for proteins, peptides and amino acids identification of biomedical importance. We, on the first try, extended its profiling application for peptidome encoded by the insect-resistance gene in rice. Taking brown planthopper (Bph) for example, we previously cloned and verified Bph32 gene, which encodes SCR ("Bph32") protein as direct executor against this injurious insect.

Nanopore detects γ-radiation inhibited HIV-1 protease activity.

Inhibition of HIV-1 protease (PR) activity is realized by exposure to Co γ-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment.