Melatonin Inhibits Endometriosis Growth via Specific Binding and Inhibition of EGFR Phosphorylation.

As a chronic gynecological disease, endometriosis is defined as the implantation of endometrial glands as well as stroma outside the uterine cavity. Proliferation is a major pathophysiology in endometriosis. Previous studies demonstrated a hormone named melatonin, which is mainly produced by the pineal gland, exerts a therapeutic impact on endometriosis.

GLUT1 exacerbates trophoblast ferroptosis by modulating AMPK/ACC mediated lipid metabolism and promotes gestational diabetes mellitus associated fetal growth restriction.

Background: Gestational diabetes mellitus (GDM) has been associated with several fetal complications, such as macrosomia and fetal growth restriction (FGR). Infants from GDM associated FGR are at increased risk for adult-onset obesity and associated metabolic disorders. However, the underlying mechanisms of GDM associated FGR remain to be explored.

Harnessing light-harvesting chlorophyll a/b-binding proteins for multiple abiotic stress tolerance in Chlamydomonas reinhardtii: Insights from genomic and physiological analysis.

Light-harvesting chlorophyll a/b-binding proteins (LHC) of photosystem II perform key functions in various processes, e.g., photosynthesis, development, and abiotic stress responses.

Tbx1 haploinsufficiency leads to local skull deformity, paraflocculus and flocculus dysplasia, and motor-learning deficit in 22q11.2 deletion syndrome.

Neurodevelopmental disorders are thought to arise from intrinsic brain abnormalities. Alternatively, they may arise from disrupted crosstalk among tissues. Here we show the local reduction of two vestibulo-cerebellar lobules, the paraflocculus and flocculus, in mouse models and humans with 22q11.

Bioinspired adaptive lipid-integrated bilayer coating for enhancing dynamic water retention in hydrogel-based flexible sensors.

While hydrogel-based flexible sensors find extensive applications in fields such as medicine and robotics, their performance can be hindered by the rapid evaporation of water, leading to diminished sensitivity and mechanical durability. Despite the exploration of some effective solutions, such as introducing organic solvents, electrolytes, and elastomer composites, these approaches still suffer from problems including diminished conductivity, interface misalignment, and insufficient protection under dynamic conditions. Inspired by cell membrane structures, we developed an adaptive lipid-integrated bilayer coating (ALIBC) to enhance water retention in hydrogel-based sensors.

Facile Preparation of High-Performance Polythiophene Derivative and Effect of Torsion Angle Between Thiophene Rings on Electrochromic Color Change.

The electrochromic phenomenon of conducting polymer is mainly dominated by the π-π* band transition. The π conjugation is influenced by the coplanarity between polymer units, deviations from which can lead to an increased ionization potential and band gap values. In order to investigate the effect of plane distortion angle on electrochromic color in the main chain structure of polymerization, high-performance poly(3,3'-dimethyl-2,2'-bithiophene) (PDMeBTh) with a large plane distortion angle is successfully synthesized in boron trifluoride diethyl etherate (BFEE) by the electrochemical anodic oxidation method.

ANK Deficiency-Mediated Cytosolic Citrate Accumulation Promotes Aortic Aneurysm.

Background: Disturbed metabolism and transport of citrate play significant roles in various pathologies. However, vascular citrate regulation and its potential role in aortic aneurysm (AA) development remain poorly understood.

Methods: Untargeted metabolomics by mass spectrometry was applied to identify upregulated metabolites of the tricarboxylic acid cycle in AA tissues of mice.

A Patching and Coding Lipid Raft-Localized Universal Imaging Platform.

Lipid rafts (LRs) are relatively well-ordered functional microdomains in cell membranes and play an irreplaceable role in physiological processes as a transduction platform for multiple signaling pathways. Due to their small size and high spatiotemporal dynamics, it is difficult to perform lipid raft-localized biomolecule imaging on the surface of living cells. Here, we report a DNA nanotechnology-based platform for reversible manipulation and localized analysis of lipid rafts, which consists of two modules: "patching and coding probe pair" and "fishing probe".

A Metalgel with Liquid Metal Continuum Immobilized in Polymer Network.

Gels are formed by fluids that expand throughout the whole volume of 3D polymer networks. To unlock unprecedented properties, exploring new fluids immobilized in polymer networks is crucial. Here, a new liquid metal-polymer gel material termed "metalgel" is introduced via fluid replacement strategy, featuring 92.

Drain versus no-drain at the donor sites of iliac crest bone graft: a retrospective study.

Background: Closed suction drainage is typically used in orthopaedic surgery to reduce the occurrence of incision complications. However, whether drainage tubes should be routinely placed in iliac crest bone graft surgeries is still unclear. This study aimed to evaluate the effect of closed suction drainage at iliac crest bone donor site on clinical prognosis.

Electrospun zeolitic imidazole framework-8 loaded silk fibroin/polycaprolactone nanofibrous scaffolds for biomedical application.

The development of electrospun nanofibrous scaffolds (NFSs) have aroused much attraction in the field of biomedical engineering, due to their small fiber diameter, high specific surface area, and excellent extracellular matrix comparability. The main focus of this study is to design and fabricate novel zeolitic imidazole framework-8 (ZIF-8)-loaded silk fibrin/polycaprolactone (SF/PCL) nanofiber composite scaffolds by using the electrospinning strategy. Firstly, ZIF-8 was synthesized and characterized, which showed remarkable features in terms of shape, size, chemical and physical properties.

Ultrasound and its coupled oak blocks treatment based on absorbed energy density for comprehensive insight and scale-up consideration of phenolic and astringency profiles in red wines.

Ultrasound and ultrasound-coupled oak blocks techniques on the phenolic and astringency profiles of Petit Verdot wines were discussed in this study. The relationship between techniques at varying absorbed energy density (AED) gradients and astringency was revealed, elucidating potential molecular mechanisms regarding compound interactions. Ultrasound was found to promote phenolics degradation and condensed tannins maturation, while oak blocks improved ellagitannins release into wines, facilitating polycondensation reactions to form larger complexes.

Comparison and Determination of the Content of Mosapride Citrate by Different qNMR Methods.

As a salt-type compound, mosapride citrate's metabolism and side effects are correlated with its salt-forming ratio. Several techniques were developed in this work to compare various quantitative nuclear magnetic resonance (qNMR) methodologies and to quantitatively examine the content of raw materials. Among the qNMR techniques, methods for H NMR and F NMR were developed.

Imaging specific proteins in living cells with small unnatural amino acid attached Raman reporters.

Fluorescence labeling fluorescent proteins (FPs) or immunofluorescence has been routinely applied for microscopic imaging of specific proteins. However, due to these over-weight and oversized labels ( GFP, 238 aa, 27 kDa, ∼4 nm in size), the potential physiological malfunctions of the target proteins are largely underestimated in living cells. Herein, for living cells, we report a small and minimally-invasive Raman reporter (about 2 aa and <1 kDa), which can be site-specifically introduced into proteins by genetic codon expansion.

Peripapillary retinal thickness and its risk factors in dry-type high myopia.

Introduction: To evaluate the alterations in the peripapillary retinal thickness and its risk factors in dry-type high myopia (HM).

Methods: One hundred and twenty eyes in 69 HM subjects were collected from March 2023 to July 2023 with the age, refractive diopter, axial length (AL), posterior scleral staphyloma, type of myopic maculopathy, and peripapillary retinal thickness. Divided into three groups according to the international photo-graphic classification system: Category 0 (C0) with no myopic retinal degenerative lesions, Category 1 (C1) with tessellated fundus, and Category 2 (C2) with diffuse chorioretinal atrophy.

regulates angiogenesis and tumor progression in hepatocellular carcinoma via the HIF-1α/VEGF pathway.

Hepatocellular carcinoma (HCC) is a highly aggressive malignant tumor with a poor prognosis. This research aimed to investigate the role of F13B in HCC and its underlying mechanisms. Through comprehensive bioinformatics analysis of the GSE120123 and The Cancer Genome Atlas (TCGA)-Liver hepatocellular carcinoma (LIHC) datasets, we identified 220 overlapping prognosis-related genes.

Phosphorylation of Piezo1 at a single residue, serine-1612, regulates its mechanosensitivity and in vivo mechanotransduction function.

Piezo1 is a mechanically activated cation channel that converts mechanical force into diverse physiological processes. Owing to its large protein size of more than 2,500 amino acids and complex 38-transmembrane helix topology, how Piezo1 is post-translationally modified for regulating its in vivo mechanotransduction functions remains largely unexplored. Here, we show that PKA activation potentiates the mechanosensitivity and slows the inactivation kinetics of mouse Piezo1 and identify the major phosphorylation site, serine-1612 (S1612), that also responds to PKC activation and shear stress.

Optimization Design of the Multidimensional Heterostructure toward Lightweight, Broadband, Highly Efficient, and Flame-Retarding Electromagnetic Wave-Absorbing Composites.

A novel multidimensional electromagnetic wave-absorbing material was developed by combining carboxylated carbon nanotubes (CNT) with graphene oxide (GO) through multidimensional design, and cobalt/nickel-based metal organic frameworks (Co/Ni-MOF) were subsequently loaded onto the GO surface via its rich functional groups to form the composite absorbing material CNT-rGO-Co/Ni-MOF. Incorporating 25 wt % of CNT-rGO-Co/Ni-MOF into the paraffin matrix led to a remarkable RL value of -43 dB at 16.4 GHz, with an effective absorbing bandwidth (EAB) exceeding 4 GHz, all within a thickness of just 1.

CIRCADIAN CLOCK-ASSOCIATED1 Delays Flowering by Directly Inhibiting the Transcription of in Pak-choi.

Flowering is critical to the success of plant propagation. The MYB family transcription factor () is an essential component of the core loop of the circadian clock and plays a crucial role in regulating plant flowering time. In this study, we found that photoperiod affects the expression pattern and expression level of , which is delayed flowering time under short-day conditions in Pak-choi [ (.

Electrochemical Activation Inducing Rocksalt-to-Spinel Transformation for Prolonged Service Life of LiMnO Cathodes.

LiMnO spinel is emerging as a promising cathode material for lithium-ion batteries, largely due to its open framework that facilitates Li diffusion and excellent rate performance. However, the charge-discharge cycling of the LiMnO cathode leads to severe structural degradation and rapid capacity decay. Here, an electrochemical activation strategy is introduced, employing a facile galvano-potentiostatic charging operation, to restore the lost capacity of LiMnO cathode without damaging the battery configuration.

Developing a mouse model of human coronavirus NL63 infection: comparison with rhinovirus-A1B and effects of prior rhinovirus infection.

Human coronavirus (HCoV)-NL63 causes respiratory tract infections in humans and uses angiotensin-converting enzyme 2 (ACE2) as a receptor. We sought to establish a mouse model of HCoV-NL63 and determine whether prior rhinovirus (RV)-A1B infection affected HCoV-NL63 replication. HCoV-NL63 was propagated in LLC-MK2 cells expressing human ACE2.