[The isolation, subculture and identification of human adipose derived endothelial progenitor cells].

Objective: To investigate the methods of isolating and identifying human adipose derived EPCs.

Methods: The cells obtained from human lipoaspirates were plated on culture dishes coated with human fibronectin and were cultured in DMEM containing 2% FBS. Cells of passage 2 cultured in EGM-2 (2% FBS) served as the induced cells (experimental group), with cells cultured in DMEM (2% FBS) as the non-induced cells (control group) .

[Pilot study of using autologous bone marrow stromal cells and coral to repair canine segmental mandibular defects].

Objective: To repair segmental mandibular defects with autologous bone marrow stromal cells (BMSCs) engineered bone.

Methods: Isolated BMSCs were expanded in vitro and osteogenic induced. In 12 canines, a 3 cm segmental mandibular defect at right mandible was created.

[Study of a novel dissoluble adhesive and its application in tissue engineering].

In order to prepare three-dimensional scaffolds with "ideal pore-structure" for tissue engineering, a novel water dissoluble adhesive was developed, and the property of adhesive as well as influence of experimental condition on bonded porogen assembly was investigated. Experimental results showed that it was possible to fabricate large dimensional porogen assembly with homogenous and controllable bonding extent by this adhesive, and a large dimensional (45 mm in diameter, 55mm in thickness) biodegradable poly(D,L-lactic acid)(PDLLA) scaffold resulting from bonded porogen was formed. The scaffolds with high porosity as well as with controllable and homogeneous inner-structure can be easily formed.

[Repair alveolar cleft bone defects with bone marrow stromal cells].

Objective: To explore the feasibility of repairing alveolar cleft bone defects with bone marrow stromal cells.

Methods: Total 7 patients of alveolar cleft were included in this study. The hBMSCs were isolated by percoll gradient centrifugation from patient's bone marrow aspirated from iliac crest.

Effective cryopreservation of human embryonic stem cells by programmed freezing.

Cryopreservation of human embryonic stem cells is an important and unsolved problem. A computer-controlled programmable cooler is used in the preservation of ES cells. Several effects have been experimentally studied, which include the cooling rate, the seeding temperature, the terminative temperature before the sample is plunged into liquid nitrogen.

Slab model studies of water adsorption and decomposition on clean and X- (X = C, N and O) contaminated Pd(111) surfaces.

To explore the effect of surface contaminants on water chemistry at metallic surfaces, adsorption and decomposition of water monomers on clean and X/Pd(111)(X = C, N and O) surfaces are investigated based on density functional theory calculations. It is revealed that H(2)O binds to Pd(111) surface primarily through the mixing of its 1b(1) with the Pd 4d(z(2)) state. A charge accumulation between the oxygen atom of water and the bound Pd atom is calculated, which is found to be relevant to the H(2)O-Pd interaction.

Cryopreservation of human embryonic stem cells: a protocol by programmed cooling.

Human embryonic stem (ES) cells have far-reaching applications in the areas of tissue engineering, regenerative medicine, pharmacology and basic scientific research. Although the culture conditions can maintain the human ES cells in an undifferentiated state for a transient period, spontaneous differentiation has also been observed during the routine culturing of ES cells. However, the maintenance of ES cells in the undifferentiated, pluripotent state for extended periods of time will be required in many areas of scientific research.

Urethral replacement using epidermal cell-seeded tubular acellular bladder collagen matrix.

Objectives: To investigate the feasibility of replacing urinary epithelium cells with foreskin epidermal cells to reconstruct engineered anterior urethra with an acellular collagen matrix.

Materials And Methods: Acellular collagen matrices were generated from allogeneic rabbit bladder submucosa. In nine rabbits, autologous foreskin epidermal cells were isolated, expanded in vitro, and labelled with 5-bromo2'-deoxy-uridine (BrdU) before seeding onto a tubular acellular collagen matrix (1.

Bone reconstruction with bone marrow stromal cells.

Bone marrow stromal/stem cells (BMSCs) are multipotent adult stem cells and have become the important cell source for cell therapy and engineered tissue repair. Their osteogenic differentiation potential has been well characterized in many in vitro studies. In addition, small animal model-based studies also reveal their capability of bone formation in vivo when implanted with biodegradable scaffold, indicating the great potential for therapeutic application.

Microvascular reconstruction of nasal ala using a reversed superficial temporal artery auricular flap.

Microsurgical technique allows successful transfer of an auricular flap in a one-stage procedure, using the root of the helix. Although a free composite auricular flap with the superficial temporal artery pedicle provides a good solution to repair nasal defects, its vascular pedicle is so limited that a vein graft from other area of the body is usually needed to reach the recipient site, leaving an unpleasant scar on the donor site. The authors present a reversed superficial temporal artery auricular free flap for alar reconstruction by microsurgical transfer.

Repair of canine mandibular bone defects with bone marrow stromal cells and porous beta-tricalcium phosphate.

Tissue engineering has become a new approach for repairing bone defects. Previous studies have been limited to the use of slow-degradable scaffolds with bone marrow stromal cells (BMSCs) in mandibular reconstruction. In this study, a 30 mm long mandibular segmental defect was repaired by engineered bone graft using osteogenically induced autologous BMSCs seeded on porous beta-tricalcium phosphate (beta-TCP, n=5).

Repair of experimental alveolar bone defects by tissue-engineered bone.

Alveolar bone resorption caused by periodontal diseases remains a difficult clinical problem to treat. Our purpose here was to develop protocols for repairing experimental horizontal alveolar bone defects. The procedure entailed isolating bone marrow stromal cells (BMSC).

In vitro tendon engineering with avian tenocytes and polyglycolic acids: a preliminary report.

Although there are many reports of in vivo tendon engineering using different animal models, only a few studies involve the short-term investigation of in vitro tendon engineering. Our previous study demonstrated that functional tendon tissue could be engineered in vivo in a hen model using tenocytes and polyglycolic acid (PGA) fibers. This current study explored the feasibility of in vitro tendon engineering using the same type of cells and scaffold material.

Repair of tendon defect with dermal fibroblast engineered tendon in a porcine model.

Harvesting autologous tenocytes for tendon engineering may cause secondary tendon defect at the donor site. Dermal fibroblasts are an easily accessible cell source and do not cause major donor site defect. This study aims to explore the possibility of tendon engineering using dermal fibroblasts.

[Repair of canine segmental mandibular defects using autogenous bone marrow stromal cells and coralline hydroxyapatite].

Objective: To repair segmental mandibular defects with autogenous bone marrow stromal cells (BMSCs) and coralline hydroxyapatite.

Methods: Isolated BMSCs were in vitro expanded and osteogenically induced. In 11 canines, a 3 cm segmental mandibular defect in right mandible was created.

Tissue-engineered bone repair of goat-femur defects with osteogenically induced bone marrow stromal cells.

Tissue engineering can generate bone tissue and has been shown to provide a better means of repairing weight-bearing bone defect. Previous studies, however, have heretofore been limited to the use of nonosteogenically induced bone marrow stromal cells (BMSCs) or the application of slow-degradation scaffolds. In this study, weight-bearing bone was engineered using osteogenically induced BMSCs.

[Effect of adeno-BMP7 transfection on osteogenesis of BMSCs].

Objective: To study the effect of adeno-BMP7 transfection on the biology of bone marrow stromal cells (BMSCs).

Methods: Bone marrow was obtained from the goat. The BMSCs were isolated and cultured at the second passage.

[Clinical study on keloid treatment with intralesional injection of low concentration 5-fluorouracil].

Objective: To explore the effect of intralesional injection of low concentration 5-fluorouracil (5-FU) on inhibition of angiogenesis of keloid.

Methods: 35 patients with 51 keloids were treated with intralesional injection of 5-FU (2-5 mg/ml) biweekly. After 3-6 times of injection, the keloids were treated with intralesional injection of corticosteroid.

[Stem cell, the basis for tissue and organ reconstruction].

Seed cells are prerequisite for reconstruction of artificial tissues/organs by tissue engineering approach. It has been widely accepted that stem cells are the best candidate for tissue engineering. The successful repair of tissue damages with adult stem cell mediated tissue engineering therapy in both animal models and patients has demonstrated the feasibility of using this technique for tissue/organ regeneration.

Tissue engineering of nearly transparent corneal stroma.

We determined whether a polyglycolic acid (PGA) scaffold bearing an adherent corneal stromal cell insert could be integrated into the ultrastructure of rabbit corneal stroma without compromising tissue transparency. Stromal cells were isolated from 10 newborn rabbits and expanded by tissue culture. After reaching confluence, the cells were harvested and mixed with nonwoven PGA fibers to form cell-scaffold constructs.

Reconstruction of the anophthalmic orbit by orbital osteotomy and free flap transfer.

It is a challenging problem for the plastic surgeon to perform adequate reconstruction of the postenucleation and radiated orbit of patient with retinoblastoma. Rebuilding of the orbital structure, reconstruction of the eye socket, and restoration of periorbital volume are required in this kind of orbital reconstruction. In this study, we reviewed 12 patients with hypoplastic orbital deformities who underwent orbital osteotomy and free flap transfer.

[Cartilage-derived morphogenetic protein 1 initiates chondrogenic differentiation of human dermal fibroblasts in vitro].

Objective: To explore the factors influencing the differentiation of fibroblasts into chondrocyte phenotype induced by a growth factor, cartilage-derived morphogenetic protein 1 (CDMP1).

Methods: Fibroblasts isolated from foreskin obtained during circumcision were cultured in the forms of micromass and monolayer culture. The culture fluid of the fibroblasts at the passage 2, 7, and 10 was added with CDMP1 of the concentrations at the concentrations of 10, 30, 100, and 300 ng/ml respectively and co-cultured for 7 days.

[Human adipose derived stem cells suppress lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli].

Objective: To study immunological properties of adipose derived stem cells (ADSC) and their in vitro immunomodulatory effects on lymphocytes.

Methods: ADSC was isolated from fat tissue by liposuction and culture expanded. Cells at passage 2 were observed for the expression of HLAI, HLAII by FACs analysis.

[Construction and clinical application of tissue engineered epidermal membrane].

Objective: To Construct tissue engineered epidermal membrane for promoting healing of clinical skin graft donor site wound.

Methods: Epidermal cells /Chitosan-Gelatin (CG) membrane was constructed with cultured human epidermal cells(EC) and CG membrane, at the donor site of split skin graft, the wound was divided into three groups: the control group was covered with CG membrane without KC, KC/CG membrane was grafted on the treatment part of the wound area, and blank group was covered with traditional vaseline gauze. The engineered epidermal membrane and its effect on wound were evaluated with gross observation, HE, immunohistochemistry, collagen type I/III ratio by picrosirius polarization method and RT - PCR test at various time points before and after operation.