Mining of the Novel Virulent ATP-Binding Cassette Importers in by Comparative Genomic Strategy.

The virulent ATP-binding cassette (ABC) importers from , the most common native multidrug resistant and emerging opportunistic pathogen in rapidly growing NTM, were explored by comparative genomic study, in view of the fact that the ABC importers of , responsible for uptaking metals, anions, amino acids, peptides, sugars, and other crucial substances from the host, had been proved to be closely related with the bacillus's virulence, survival in the host macrophages, antibiotic resistance, modulation of host immune system, and so on, although detailed mechanism was unclear. For virulent ABC importers from predicted by orthology and phylogeny analysis of nucleotide-binding domains (NBDs) of , , and , the antibiotic susceptibility of overexpression transformant and knockout mutant was assayed after confirmation by experiment. Three-domain importers were dominant ones in (60.

Identification of peptide biomarkers for authentication of Atlantic salmon and rainbow trout with untargeted and targeted proteomics approaches and quantitative detection of adulteration.

Atlantic salmon is often adulterated or substituted by rainbow trout with much lower price and quality. However, it is extremely difficult to distinguish Atlantic salmon and rainbow trout due to their similar appearance and close relationship in species. In the present work, untargeted and targeted proteomics approaches were both implemented to identify species-specific peptide biomarkers of Atlantic salmon and rainbow trout.