Optimizing office building performance in the HSWW region of China using simulation with Hyperopt CatBoost and SPEA2.

At present, the evaluation of the comprehensive performance of urban office buildings remains an area of significant discussion. This research aims to optimize the building performance of office buildings in the hot summer and warm winter (HSWW) region, focusing on three key aspects: energy use intensity (EUI), useful daylight illuminance (UDI), and percentage of thermal comfort (PTC). The study employs the Hyperparameter Optimization (Hyperopt)-Categorical Boosting (CatBoost)-Strength Pareto Evolutionary Algorithm 2 (SPEA2) multi-objective optimization method, generating 3,000 datasets via Latin Hypercube Sampling (LHS).

Profilin 1 induces drug resistance by downregulating CD138 expression via autophagy in multiple myeloma.

Multiple myeloma (MM), a plasma cell-derived malignant hematological disease, is often treated with bortezomib, a highly effective first-generation proteasome inhibitor. However, resistance to bortezomib is a common occurrence. Profilin 1 (PFN1), a cytoskeleton-related gene known to promote autophagy in MM, induces this resistance to bortezomib, but it is unclear why.

ELF1's Role in Colorectal Cancer: Up-Regulating DCLK1 Expression to Propel Malignant Progression and Stemness Features.

Background: Exploring the pathological mechanism of colorectal cancer (CRC) onset and advancement is critical to clinical diagnosis and treatment. In this context, our study brings a novel perspective by investigating the role and regulatory mechanism of E74 Like ETS Transcription Factor 1 () in CRC, a topic that has not been extensively explored.

Methods: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blotting (WB) assays were used to detect the expression of ELF1 in CRC cells.

Effects of practical models of low-volume high-intensity interval training on glycemic control and insulin resistance in adults: a systematic review and meta-analysis of randomized controlled studies.

Objectives: The aim of this systematic review and meta-analysis was to investigate the effects of practical models of low-volume high-intensity interval training protocols (LV-HIIT) on glucose control and insulin resistance compared with moderate-intensity continuous training (MICT) protocols and no-exercise controls (CON).

Methods: Four databases (PubMed, Web of Science, Scopus, and Cochrane Library) were searched for randomized controlled studies conducted using LV-HIIT interventions (HIIT/SIT protocols involving ≤ 15 min of intense training, within a session lasting ≤ 30 min; < 30 s all-out sprint for SIT additionally). The inclusion criteria required glucose and insulin resistance markers to be evaluated pre- and post-intervention among adults who were not trained athletes.

Probiotic domestication and engineering enable one-shot treatment for bladder mucosal repair.

The bladder mucosa is an important blood-urine barrier in the human body, its destruction can lead to distressing cystitis. Traditional treatment approaches often require frequent catheterization and intravesical instillation of hyaluronic acid (HA), which greatly reduces patient compliance and therapeutic efficacy. Herein, we develop a probiotic-based one-shot therapy to repair bladder mucosa with improved convenience, efficacy, and biosafety.

Development of a colloidal gold immunochromatographic assay utilizing dual-antibody sandwich method for detecting .

A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of () antigens in the early stages of infection. Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. Colloidal gold and polyclonal antibody-colloidal gold complexes were synthesized under optimized conditions.

Early intervention with pericyte Fli-1 post-TBI attenuates hippocampal BBB disruption and subsequent neuroinflammation with neurological deficits.

The ETS transcription factor Fli-1, known for regulating the vitality of pericyte in mice, has not been thoroughly investigated in traumatic brain injury (TBI). In this study, we used a mouse TBI model to demonstrate that Fli-1 expression in pericyte within the ipsilateral hippocampus is significantly increased following TBI and is associated with pericyte loss. Interfering with Fli-1 expression in pericyte disrupted their interactions with microglia, which in turn inhibited the transformation of microglia to a pro-inflammatory phenotype.

Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1.

Background: A close relationship exists between castration-resistant prostate cancer (CRPC) and histidine metabolism by gut microbes. However, the effects of the histidine metabolite imidazole propionate (IMP) on prostate cancer (PCa) and its underlying mechanisms are not well understood.

Methods: We first assessed the effects of IMP on cell proliferation and migration at the cellular level.

Association between gout and kidney stone: results from mendelian randomization and the NHANES study.

Background: Kidney stones are a common urologic disease with an increasing incidence year by year, and there are similar influences between gout status and kidney stone incidence. Therefore the contribution of gout status to the incidence of kidney stones is unclear. The aim of this study was to investigate the relationship between gout status and kidney stones and to further explore the causal relationship by Mendelian randomization (MR) analysis.

Collective dynamics of swarmalators driven by a mobile pacemaker.

Swarmalators, namely, oscillators with intrinsic frequencies that are able to self-propel to move in space, may undergo collective spatial swarming and meanwhile phase synchronous dynamics. In this paper, a swarmalator model driven by an external mobile pacemaker is proposed to explore the swarming dynamics in the presence of the competition between the external organization of the moving pacemaker and the intrinsic self-organization among oscillators. It is unveiled that the swarmalator system may exhibit a wealth of novel spatiotemporal patterns including the spindle state, the ripple state, and the trapping state.

Nicorandil restores endothelial cell Kir6.2 expression to alleviate neuropathic pain in mice after chronic constriction injury.

The clinical management of neuropathic pain (NP) remains a significant challenge, as current pharmacological treatments do not fully meet clinical needs. Nicorandil, a potassium ATP channel agonist widely used in cardiovascular medicine, has recently been shown to have significant potential for analgesia. This study aimed to investigate the effects and mechanisms of nicorandil in a chronic constriction injury (CCI) mouse model.

Engineering a novel pathway for efficient biosynthesis of salicin in Escherichia coli.

Salicin is a natural glycoside compound widely used to treat fever, inflammation, and analgesia. Currently, salicin is primarily extracted from willow bark, which is not only cumbersome in terms of extraction and separate steps, but also subject to seasonal and geographic limitations. In this study, a highly efficient biosynthetic pathway for salicin synthesis was designed and constructed in E.

Soluble Polyimide Coated UHMWPE Fibers with Multiple Property Enhancements: Surface Activity, Tensile Strength, Heat Resistance, Acid Resistance, and Erasability.

Ultrahigh molecular weight polyethylene (UHMWPE) fibers possess excellent mechanical properties, yet their applications are severely limited by surface inertness and low melting points. To enhance surface activity and temperature resistance, soluble polyimide (PI) is applied to the surface of UHMWPE fibers. A mussel-inspired biomimetic polycatechol/polyamine (PA) coating is initially constructed on the UHMWPE fiber surface by oxidative self-polymerization, serving as a secondary reaction platform.

RAGE mediates hippocampal pericyte responses and neurovascular unit lesions after TBI.

Traumatic brain injury impairs brain function through various mechanisms. Recent studies have shown that alterations in pericytes in various diseases affect neurovascular function, but the effects of TBI on hippocampal pericytes remain unclear. Here, we investigated the effects of RAGE activation on pericytes after TBI using male C57BL/6 J mice.

Molecular basis for the activation of human spliceosome.

The spliceosome executes pre-mRNA splicing through four sequential stages: assembly, activation, catalysis, and disassembly. Activation of the spliceosome, namely remodeling of the pre-catalytic spliceosome (B complex) into the activated spliceosome (B complex) and the catalytically activated spliceosome (B complex), involves major flux of protein components and structural rearrangements. Relying on a splicing inhibitor, we have captured six intermediate states between the B and B complexes: pre-B, B-I, B-II, B-III, B-IV, and post-B.

Comparing the Efficacy of Antiosteoporotic Drugs in Preventing Periprosthetic Bone Loss Following Total Hip Arthroplasty: A Systematic Review and Bayesian Network Meta-Analysis.

Background: Periprosthetic bone loss is a well-known phenomenon following total hip arthroplasty (THA). However, the choice of drugs for prevention remains controversial. Therefore, the aim of this study was to determine the best drug to treat periprosthetic bone loss by comparing changes in bone mineral density (BMD) at different times after THA.

Non-Line-of-Sight Imaging and Vibrometry Using a Comb-Calibrated Coherent Sensor.

Non-line-of-sight (NLOS) imaging has the ability to reconstruct hidden objects, allowing a wide range of applications. Existing NLOS systems rely on pulsed lasers and time-resolved single-photon detectors to capture the information encoded in the time of flight of scattered photons. Despite remarkable advances, the pulsed time-of-flight LIDAR approach has limited temporal resolution and struggles to detect the frequency-associated information directly.

Broadening Eligibility Criteria and Diversity among Patients for Cancer Clinical Trials.

Background: Certain populations have been historically underrepresented in clinical trials. Broadening eligibility criteria is one approach to inclusive clinical research and achieving enrollment goals. How broadened trial eligibility criteria affect the diversity of eligible participants is unknown.

Response- and Progression-Based End Points in Trial and Observational Cohorts of Patients With NSCLC.

Importance: Response Evaluation Criteria in Solid Tumors (RECIST) are commonly used to assess therapeutic response in clinical trials but not in routine care; thus, RECIST-based end points are difficult to include in observational studies. Clinician-anchored approaches for measuring clinical response have been validated but not widely compared with clinical trial data, limiting their use as evidence for clinical decision-making.

Objective: To compare response- and progression-based end points in clinical trial and observational cohorts of patients with non-small cell lung cancer (NSCLC).

Structural insights into branch site proofreading by human spliceosome.

Selection of the pre-mRNA branch site (BS) by the U2 small nuclear ribonucleoprotein (snRNP) is crucial to prespliceosome (A complex) assembly. The RNA helicase PRP5 proofreads BS selection but the underlying mechanism remains unclear. Here we report the atomic structures of two sequential complexes leading to prespliceosome assembly: human 17S U2 snRNP and a cross-exon pre-A complex.

Bleeder's Femur: The Proximal Femoral Morphology in Hemophilic Patients Who Underwent Total Hip Arthroplasty.

Introduction: Patients with hemophilia (PWH) constantly suffer hemarthrosis, which leads to deformity of the hip joint. Therefore, PWH who are going to receive total hip arthroplasty (THA) should be exclusively treated before the surgery with careful measurement of their proximal femur. Hence, we conducted a retrospective study to explore the anatomical parameters of and differences in the proximal femur in hemophilic patients who underwent THA.

Oriented assembly of hydrophilic nanochains modified by porous zirconium-based coordination polymers for glycopeptides analysis.

Mass spectrometry (MS)-based glycoproteomics research requires additional sample pretreatment to improve the effective identification of low-abundance glycopeptides without interference from non-glycoproteins. Herein, an attractive strategy using resorcinol-formaldehyde (RF) resin and zirconium-based coordination polymer (Zr-BCP) was established to prepare one-dimensional porous coordination polymer composites for glycopeptide enrichment before MS analysis. The obtained FeO@RF@Zr-BCP nanochains feature excellent magnetic response (42.

Long-term spatiotemporal variations in surface NO for Beijing reconstructed from surface data and satellite retrievals.

Remote sensing data from the Ozone Monitoring Instrument (OMI) and the TROPOspheric Monitoring Instrument (TROPOMI) play important roles in estimating surface nitrogen dioxide (NO), but few studies have compared their differences for application in surface NO reconstruction. This study aims to explore the effectiveness of incorporating the tropospheric NO vertical column density (VCD) from OMI and TROPOMI (hereafter referred to as OMI and TROPOMI, respectively, for conciseness) for deriving surface NO and to apply the resulting data to revisit the spatiotemporal variations in surface NO for Beijing over the 2005-2020 period during which there were significant reductions in nitrogen oxide emissions. In the OMI versus TROPOMI performance comparison, the cross-validation R values were 0.

Twin-Field Quantum Key Distribution without Phase Locking.

Twin-field quantum key distribution (TF-QKD) has emerged as a promising solution for practical quantum communication over long-haul fiber. However, previous demonstrations on TF-QKD require the phase locking technique to coherently control the twin light fields, inevitably complicating the system with extra fiber channels and peripheral hardware. Here, we propose and demonstrate an approach to recover the single-photon interference pattern and realize TF-QKD without phase locking.