BL02, a phage against carbapenem- and polymyxin-B resistant Klebsiella pneumoniae, isolated from sewage: A preclinical study.

The emergence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) represents a threat to public health. Polymyxin-B is generally considered a last-resort antibiotic. In this study, we isolated a carbapenem- and polymyxin-B resistant K.

Early application of continuous high-volume haemofiltration can reduce sepsis and improve the prognosis of patients with severe burns.

Background: In the early stage of severe burn, patients often exhibit a high level of inflammatory mediators in blood and are likely to develop sepsis. High-volume haemofiltration (HVHF) can eliminate these inflammatory mediators. We hypothesised that early application of HVHF may be beneficial in reducing sepsis and improving the prognosis of patients with severe burns.

Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered gene aberrant methylation.

Severe burns may lead to intestinal inflammation and oxidative stress resulting in intestinal barrier damage and gut dysfunction. In the management of severe burns, therapies are needed to attenuate whole-body inflammatory responses and control the burden of oxidative stress. In this study, we evaluated the effects of oral glutamine (Gln) with probiotics on burn-induced intestinal inflammation and oxidative stress using a Wistar rat burn injury model.

CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury.

The mechanism underlying pulmonary inflammation in thermal inhalation injury remains elusive. Cystic fibrosis, also hallmarked with pulmonary inflammation, is caused by mutations in CFTR, the expression of which is temperature-sensitive. We investigated whether CFTR is involved in heat-induced pulmonary inflammation.

A randomized and controlled multicenter prospective study of the Chinese medicinal compound Fufang Xuelian Burn Ointment for the treatment of superficial and deep second-degree burn wounds.

The objective of this study was to evaluate the efficacy and safety of a traditional Chinese medicine, Fufang Xuelian Burn Ointment (FXBO), to treat superficial and deep second-degree burn wounds. A four-center, randomized, controlled, and prospective study was conducted. Overall, 240 patients with either superficial or deep second-degree burn wounds were enrolled consecutively in this study.

[A propose to suspend the use of hydroxyethyl starch for fluid resuscitation in shock phase of severe burns].

Based on the result of randomized controlled trials and meta-analysis recently, the infusion of hydroxyethyl starch (HES) was not shown to over match routine crystalline solution in exerting resuscitation effect against hypovolemia of patients with burn shock, severe systematic infection, or other critical conditions, on the other hand, it may induce renal toxicity and other toxic and side effects. Since the pathological mechanism underlying hypovolemia during shock phase after burn is similar to that of severe systemic infection, we propose to suspend the use of HES for fluid resuscitation during the shock phase of severe burn until further elucidation.

[Influence of infection of murine chemokine receptor-7 recombinant lentivirus on the immunogenicity and migration of DC 2.4 cells].

Objective: To observe the influence of infection of murine chemokine receptor-7 recombinant lentivirus on the immunogenicity and migration of dendritic cell strain DC 2.4 cells.

Methods: DC 2.

[Clinical characteristics and diagnosis of sepsis in pediatric burn patients].

Pediatric burn patients account for more than 1/3 of the inpatients in the same period, and its incidence surpasses that of burn patients in other age groups. However, it brings about much difficulty to treat pediatric burn patients complicated by sepsis, which brings a significantly higher mortality than that of the adult. Moreover, the physiological characteristics, development of organs, drug metabolism, and body response to burn injury in children are obviously different from those of the adult.

Relation between proteinuria and acute kidney injury in patients with severe burns.

Introduction: Proteinuria in burn patients is common, and may be associated with acute kidney injury (AKI) and adverse outcomes. We evaluated the incidences, outcomes, characteristics and determinants of proteinuria and its influence on AKI and outcomes in burn patients.

Methods: This retrospective study was carried out in a hospital's burn department.

[Reproduction of a rat model of burn with infection].

Objective: To reproduce a reliable rat model of burn with infection for the study of prevention and treatment of infected wound.

Methods: (1) Electrical burn producing apparatus equipped with constant temperature (80°C) and pressure (0.5 kg) was used to reproduce burn injury (with area of 4.

HSP70 protects intestinal epithelial cells from hypoxia/reoxygenation injury via a mechanism that involves the mitochondrial pathways.

Though recent studies have reported the importance of several endogenous cytoprotective factors including heat shock protein 70 (HSP70) that protect intestinal epithelial cells (IECs) from the effects of stress and injury, the exact mechanism of HSP70 underlying cytoprotection against hypoxia/reoxygenation induced IEC injury remains unclear. The present study was designed to investigate the possible mechanisms by which HSP70 protected IECs against hypoxia/reoxygenation injury and focused on the effects of HSP70 on IEC apoptosis induced by hypoxia/reoxygenation injury. Recombinant adenoviruses (Ad-HSP70) were transfected into the intestinal epithelial cell line in vitro and then suffered from 90 min of hypoxia followed by 60 min of reoxygenation.

[Clinical assessment of colistin in treating infections caused by multidrug-resistant gram-negative bacillus in patients with severe burn].

Objective: To investigate the therapeutic effect and side effects of colistin in treating infections caused by multidrug-resistant (MDR) gram-negative bacillus in patients with severe burn in order to provide the basis for reasonable application of this antibiotic in clinic.

Methods: Nine burn patients suffered from infections caused by MDR gram-negative bacillus admitted to our institute from August 2005 to January 2009 were involved in this study. On the premises that isolated bacteria were only sensitive to colistin or not sensitive to other antibiotics, patients were treated with intravenous drip of colistin (100 x 10(4) - 150 x 10(4) U/d), or intravenous drip combined with administration of the drug into respiratory tract by atomization or instillation (50 x 10(4) - 100 x 10(4) U/d).

[A clinical study of fungal infection in burn patients].

Objective: To address the features of the fungal infection after burn injury in clinic.

Methods: Three thousand nine hundred and nine burn patients admitted to our institute from Jan. 2003 to Dec.

In vitro maturation and migration of immature dendritic cells after chemokine receptor 7 transfection.

Dendritic cells are specialized antigen-presenting cells that regulate immunity and tolerance. Chemokine receptor 7 (CCR7), which is expressed by mature dendritic cells, mediates the migration of the cells to secondary lymphoid organs and thus regulates immune responses. It has been demonstrated that immature dendritic cells can induce immune tolerance, but they do not express CCR7 and cannot migrate to secondary lymphoid organs.

[Clinical practice and evaluation of relative fluid resuscitation formula at burn shock stage].

Objective: To evaluate the application of the Third Military Medical University (TMMU) formula for fluid resuscitation on the major burn patients during shock stage.

Methods: Seventy-one thermal injury patients (burn area more than 30% TBSA, without especial illness, hospitalization within 8 hour after burn) admitted from 2005 to 2007 were divided into adult group (n = 46), child group (n = 25). Fluid resuscitation was initiated as per the TMMU formula.

[Influence of HSP70 on function and energy metabolism of mitochondria in intestinal epithelial cells after hypoxia/reoxygenation].

Objective: To investigate the effect of recombinant adenovirus-mediated heat shock protein 70 (HSP70) on energy metabolism of mitochondria in intestinal epithelial cells (IEC-6) after hypoxia/reoxygenation injury .

Methods: IEC-6 cells were transfected with HSP70 recombinant adenovirus vectors (Ad-HSP70) and empty adenovirus vectors. The expression of HSP70 protein was detected by Western blotting.

[Study on the induction of skin transplantation tolerance against rejection in mice by third-party dendritic cells loaded with donor's antigens].

Objective: To investigate whether the third-party dendritic cells (DC) incubated with donor's antigens possess the similar immune functions with donor derived immature DC.

Methods: Female C57 BL/6, BALB/c and Kunming mice were used as skin transplant donors, recipients and third-party, respectively. Forty BALB/c mice were randomly divided into A (normal control), B (cyclophosphamide administration), C (CTX and donor derived immature DC), D (CTX and third-party immature DC), E (CTX and third-party DC loaded with donor's antigens) groups, with 8 mice in each group.

[Standardized definitions and diagnostic criteria for infection in burn patients].

There are many criteria for the diagnosis of infection and sepsis in most patients, but the standardized definitions for infection and sepsis in burn patients are less applicable to the burn population and have never been developed. We recommend that suspicious systemic infection (sepsis) should be considered as a clinical syndrome defined by the presence of signs and symptoms of systemic infection even with negative blood microbial culture, systemic infection should be identified with positive blood microbial culture or clinical response to antimicrobials. We also expand the list of diagnostic criteria for systemic infection to reflect clinical experience in burn patients.

[Influence of heat shock protein 90 on protein serine threonine kinases expression in hypoxic cardiomyocytes].

Objective: To explore the effect of endogenous heat shock protein 90 (HSP90) on the AKT signaling pathway of hypoxic cardiomyocytes.

Methods: The hypoxia model of neonatal rat cardiomyocyte was established. The cells were randomly divided into normal control, hypoxia, Geldanamycin (GA, with hypoxia after Geldanamycin treatment) groups.

Induction of heat shock protein 70 by sodium arsenite attenuates burn-induced intestinal injury in severe burned rats.

The present study was designed to assess the effects of induced heat shock protein 70 (HSP70) on intestinal injury after severe burn. Wistar rats were randomly divided into four groups: control group, burn group (B group), sodium arsenite pretreatment group (SA group), and sodium arsenite+quercetin pretreatment group (SA+Qu group). Plasma endotoxin and d-lactic acid content were determined at 3, 6, 12, 24, and 48h after severe burn.

[Influence of adenovirus transfection on the maturation characteristics of human immature dendritic cells].

Objective: To observe the changes in the phenotype characteristics and immune function after transfection of cord blood derived immature dendritic cells( imDC) with Adeasy-EGFP adenovirus vector, and to explore the function of IL-10 in inhibition of imDC maturation.

Methods: Immature dendritic cells were generated from human cord blood(CB) monocyte cultured with rhGM-CSF and rhIL-4. The recombinant adenovirus vector AdEASY-EGFP was transduced into immature dendritic cells on the third day with or without adding IL-10.

[Experimental study on cryopreservation of immature dendritic cells derived from cord blood].

Objective: To investigate the biological properties of immature dendritic cells( imDC) derived from cord blood before and after cryopreservation, so as to provide a method for preservation of imDC.

Methods: Immature dendritic cells were generated from human cord blood (CB) monocytes and cultured with rhGM-CSF and rhIL-4, and 10% DMSO was added into culture medium as cryopreservation reagent. After freezing in - 80 degrees C refrigerator, the cells were finally cryopreserved in - 196 degrees C liquid nitrogen, and then thawed with 40 'C water, and they were finally named frozen-thawed imDC.

[The influence of antigen loading on the immunological characteristics of dendritic cells induced by low concentrations of granulocyte macrophage colony stimulating factor].

Objective: To investigate the influence of low doses of granulocyte macrophage colony stimulating factor on the allogeneic antigen (Ag) ingestion capacity of immature dendritic cells (GM low DC), and subsequently the changes in the cellular phenotype and function.

Methods: Mononuclear cells from C57BL/6 mice was labelled with 3H-Leu to make Ag supernatant. The Ag supernatant was cocultured with GM low DC or mature DC for 30,60 and 90 mins, then cpm value were determined.