[Experimental study on influence of bone marrow mesenchymal stem cells on activation and function of mouse peritoneal macrophages].

Objective: To explore the influence of bone marrow (BM) mesenchymal stem cells (MSCs) on macrophage activation after lipopolysaccharide (LPS) stimulation.

Methods: Mouse BM MSCs were isolated and purified by adherence screening, and mouse peritoneal macrophages (MPM) were collected by sodium thioglycollate peritoneal injection, and the co-culture system was established by planting macrophages on the MSCs monolayer. The grouping of experiments: group A: MPM; group B: MPM + LPS; group C: MPM + LPS + MSC; group D: MPM + LPS + MSC supernatant.

[Effects of mesenchymal stem cells on proliferation and apoptosis of allogeneic B lymphocytes].

Objective: To study the influence of bone marrow mesenchymal stem cells (MSC) on the proliferation and apoptosis of allogeneic peripheral B lymphocytes in vitro.

Methods: MSCs were isolated from the peripheral blood of healthy volunteer and cultured. B lymphocytes were isolated from another healthy volunteer and co-cultured with the MSCs at the B:MSC ratios of 50:1, 10:1, and 1:1 or with different concentrations of MSC supernatant (12.