[Distribution of genetic polymorphisms of serum amyloid protein A1 in healthy Chinese Han and Uighur populations in Xinjiang].

Objective: To investigate the genetic polymorphisms of rs2229338 and rs12218 loci of serum amyloid protein A1 (SAA1) gene in healthy Chinese Han and Uighur populations of Xinjiang.

Methods: The genotypes of the SAA1 gene were detected in 316 Uighur and 362 Han healthy individuals by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).

Results: The genotype distributions of both populations were in the Hardy-Weinberg equilibrium (both P>0.

[Incidence and distributing feature of chronic heart failure in adult population of Xinjiang].

Objective: To investigate the prevalence and distributing feature of chronic heart failure (CHF) in adult population of Xinjiang.

Methods: Four-stage random sampling method was used to analyze the prevalence and distributing feature of self-reported congestive heart failure among different nationalities in adult (35 years and over) population of Xinjiang. Sampling was collected from 6 localities (Urumqi, Kelamayi, Fukang, Turfan Basin, Hetian, Yili Hazakh).

Association of interaction between smoking and CYP 2C19*3 polymorphism with coronary artery disease in a Uighur population.

Objectives: Cytochrome P450 (CYP) 2C19 is expressed in vascular endothelium and metabolizes arachidonic acid to biologically active epoxyeicosatrienoic acids (EETs), which are potent endogenous vasodilators and inhibitors of vascular inflammation. The purpose of this study is to explore the relationship between the interaction of CYP2C19*3 polymorphism and smoking and coronary artery disease (CAD) in a Uighur population.

Methods: In a Chinese Uighur case-control study of patients with CAD (n = 336) and healthy controls (n = 370), we investigated the roles of polymorphism in the CYP2C19 gene by the use of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis.

[Construction and expression of a recombinant adenovirus with hEPO].

Aim: To construct the adenovirus vector containing human erythropoietin gene (hEPO) and to detect its expression in HeLa cells.

Methods: hEPO gene was subcloned into the shuttle plasmid pAdTrack-CMV and then a two-step transformation procedure was employed to construct a recombinant adenoviral plasmid with hEPO. The recombinant adenoviral plasmid with hEPO was digested with Pac I and then transfected into HEK293 cells to package recombinant adenovirus particles.

[Outcome of percutaneous balloon pulmonary valvuloplasty for patients with pulmonary valve stenosis].

Objective: To assess the effects of percutaneous balloon pulmonary valvuloplasty (PBPV) for patients with pulmonary valve stenosis (PS).

Methods: From February 1996 to March 2003, 65 patients with isolated PS were diagnosed by echocardiography and received PBPV in our department, clinical data were analyzed in this study.

Results: Age of 65 patients ranged from 1 to 48 years [mean (13.

[Association between wnt signal pathway and post-infarction cardiac remodeling/rupture in aged mice].

Objective: To observe the association between wnt signal pathway and post infarction left ventricular remodeling/rupture in mice with various ages.

Methods: Three months-old (young group, n = 116) and 18 months-old (aged group, n = 116) male C57/BL mice were studied. Seventy mice underwent ligation of left coronary artery, 10 sham-operation and echocardiography and hemodynamics were performed 7 d post-infarction, 36 infarcted mice were used for detecting expression of dvl-1, beta-catenin and connexin 43 in left ventricular (LV) myocardium and infarction region at 3 d, 7 d, 14 d post infarction (n = 12 each).

[Recombinant adeno-associated virus serotype 9 transfection of rats H9C2 cells in vitro].

Aim: To evaluate the transfection efficiency using recombinant adeno-associated virus Serotype 9 mediated enhanced green fluorescent protein (rAAV9- EGFP) to rats H9C2 cells and the impact on growth of H9C2 cells.

Methods: rAAV9-EGFP was transfected into H9C2 cells at different multiplicities of infection (MOI=1 x 10(5), 1 x 10(6), 1 x 10(7)). EGFP expression in the cells was observed under inverted fluorescence microscope, and the EGFP-positive cell percentage determined by flow cytometry.

[Association on the haplotypes of CYP4F2 gene and myocardial infarction].

Objective: The aim of this study was to assess the association between human CYP4F2 gene and myocardial infarction (MI), using a haplotype-based case-control study. A separate analysis on gender was also carried out.

Methods: There were 250 MI patients and 250 control subjects genotyped for 5 SNPs of the human CYP4F2 gene (rs3093105, rs3093135, rs1558139, rs2108622, rs3093200).

Association of the purinergic receptor P2Y, G-protein coupled, 2 (P2RY2) gene with myocardial infarction in Japanese men.

Background: Atherosclerosis leads to myocardial infarction (MI) and P2RY2 plays an important role in this process. The aim of the present study was to investigate the association between human P2RY2 and MI via a haplotype-based case-control study that additionally analyzed the group by sex.

Methods And Results: The 310 MI patients and 254 controls were genotyped for 5 single-nucleotide polymorphisms (SNPs) of the human P2RY2 gene (rs4944831, rs1783596, rs4944832, rs4382936, rs10898909).

[Haplotypes analysis of the prostacyclin synthase gene and myocardial infarction in Uigur population].

Objective: To investigate the association between the polymorphisms and haplotypes of prostacyclin synthase gene with MI in Uigur patients in Xinjiang.

Methods: 210 patients with MI and 206 healthy control subjects were genotyped for 3 SNPs of the human prostacyclin synthase gene by polymerase chain reaction and restriction fragment length polymorphism.

Results: The genotype distributions of the control group and MI group were in the Hardy-Weinberg equilibrium (both P > 0.

[Ischemic postconditioning protects hypertrophic myocardium by ERK1/2 signaling pathway: experiment with mice].

Objective: To investigate the effects of ischemic postconditioning (IPost) in protecting hypertrophic myocardium subjected to ischemic-reperfusion (I/R) and to study the role of extracellular regulated protein kinase (ERK1/2) in mediating such protection.

Methods: Transverse aortic constriction (TAC) operation was performed on 12-week-old C57/BL mice to establish left ventricular hypertrophy models. Sixty-four isolated TAC mouse hearts were mounted onto the Langendorff perfusion system and randomly divided into 4 equal group: (1) I/R group undergoing stable perfusion for 30 min, ischemia for 30 min, and re-perfusion for 120 min (an I/R cycle) to cause hypertrophic myocardium I/R injury, (2) IPost group undergoing ischemia for 10s and reperfusion for 10s, totally 3 cycles (60s) before reperfusion for 120 min, (3) I/R+ PD98059 (an ERK1/2 inhibitor) group undergoing perfusion of Krebs-Henseleit (KH) buffer with PD98059 10(-5)mol/L for 15 min and perfusion of KH buffer without PD98059 at the beginning of re-perfusion, and (4) IPost + PD98059 group undergoing 3 cycles of IPost and perfusion of KH buffer with PD98059 10(-5)mol/L for 15 min at the beginning of re-perfusion.

Downregulation of survival signalling pathways and increased apoptosis in the transition of pressure overload-induced cardiac hypertrophy to heart failure.

1. Transition from compensated left ventricular (LV) hypertrophy to decompensated heart failure was characterized using a pressure-overload induced model to elucidate the temporal relationship between cardiomyocyte apoptosis and survival signalling in this transition. 2.

[Association of GLu461ALa polymorphism of prostacyclin synthase gene with myocardial infarction in Uigur population].

Objective: To investigate the association between the polymorphism of prostacyclin synthase gene (CYP8A1) and myocardial infarction (MI) in Uigur population.

Methods: Totally 210 patients with MI and 206 healthy control subjects were detected by polymerase chain reaction and restriction fragment length polymorphism. The serum 6-keto-PGF(1alpha) was detected with radioimmunoassay kit in all subjects.

Association of polymorphisms of PTGS2 and CYP8A1 with myocardial infarction.

Background: Cyclooxygenase-2 (COX-2) and prostacyclin synthase (PGIS) are enzymes involved in prostaglandin and prostacyclin synthesis, which have been linked to cardiovascular disease risk. We hypothesized that genetic variations altering the function of these enzymes would modify the risk of myocardial infarction (MI).

Methods: In a Chinese case control study of MI patients (n=356) and healthy controls (n=350), we investigated the roles of polymorphisms in the PGIS gene (CYP8A1) and the COX-2 gene (PTGS2) using polymerase chain reaction-restriction fragment length polymorphism analysis.

[Change of tissue factor pathway ratio during attack in patients with acute myocardial infarction and its clinical significance].

This study was aimed to investigate the change of tissue factor pathway (TFP) ratio during the attack of acute myocardial infarction (AMI) and its clinical significance. Plasma recalcification time was assayed by manual operation. Plasma tissue factor (TF), TF pathway inhibitor (TFPI) antigen, FVII:Ag, activated FVII (FVIIa) and D-Dimer were measured by enzyme linked immunoabsorbent assay (ELISA).

Haplotype analysis of the CYP8A1 gene associated with myocardial infarction.

Objective: The aim of this study was to assess the association between the human CYP8A1 gene and myocardial infarction (MI) in Chinese people.

Methods: 210 MI patients and 206 age-matched controls were genotyped and constructed haplotypes for 3 SNPs [3982C>T (rs5602), C1117A (rs5629), C251T (rs454-98106)] of the human CYP8A1 gene.

Results: The CC genotype of rs5629 was more frequently in MI patients than in control subjects (P = .

[Effects of ischemia postconditioning on ischemia-reperfusion injury and reperfusion injury salvage kinase signal transduction pathways in isolated mouse hearts].

Objective: To explore the effects of ischemia postconditioning (IPC) on ischemia-reperfusion (I/R) injury and associated reperfusion injury salvage kinase (RISK) signal transduction pathways changes in isolated mouse hearts.

Methods: Langendorff perfused C57/BL mouse hearts were divided to 6 groups: (1) control: 30 min global ischemia and 2 h reperfusion (I/R); (2) IPC with 3 episodes, IPC with 3 episodes of 10 s of ischemia and 10 s reperfusion after 30 min ischemia and before 2 h reperfusion; (3) IPC with 6 episodes, IPC with six episodes of 10 s of ischemia and 10 s reperfusion after 30 min ischemia and before 2 h reperfusion; (4) delayed IPC, IPC with 3 episodes of 10 s of ischemia and 10 s reperfusion after 30 min ischemia and at one minute after reperfusion; (5) IPC + ERK1/2 inhibitor PD98059 (10(-5) mol/L for 15 min); (6) I/R + ERK1/2 inhibitor PD98059 (10(-5) mol/L for 15 min). The effects of IPC on hemodynamics, coronary artery flow, creatine kinase (CK) and lactate dehydrogenase (LDH) release, myocardial SOD, MDA, phospho-protein kinase (P-ERK1/2) and phospho-protein kinase B (P-Akt) as well as myocardial infarction size were measured.

[Study on the association of cyclooxygenase-2 -765g>C and prostacyclin synthase C1117A polymorphisms and the risk of myocardial infarction in Uigur population of Xinjiang, China].

Objective: The purpose of this study was to investigate the association of genetic polymorphism of cyclooxygenase-2 and prostacyclin synthase with myocardial infarction (MI) in Uigur population in Xinjiang.

Methods: 178 patients with MI and 175 healthy control subjects were detected on the genetic polymorphism of cyclooxygenase-2 and prostacyclin synthase by polymerase chain reaction-based restriction fragment length polymorphism. Other serum 6-keto-PGF1alpha concentration and biochemical indicators were detected in all the subjects.

Percutaneous transcatheter closure of patent ductus arteriosus with an amplatzer duct occluder using retrograde guidewire-established femoral arteriovenous loop.

1. The traditional antegrade wire-guided percutaneous transcatheter approach is not ideal in closing some types of patent ductus arteriosus (PDA) with abnormal morphology. The aim of the present study was to evaluate the efficacy of a retrograde wire-guided transcatheter approach for closure of some types of PDA using the Amplatzer duct occluder (ADO).

Preparation of monoclonal antibodies against human ventricular myosin light chain 1 (HVMLC1) for functional studies.

Using purified recombinant human ventricular myosin light chain 1 (HVMLC1) as the antigen, three monoclonal antibodies, designated C8, C9 and B12, were prepared. Immunoblot experiments demonstrated that all monoclonal antibodies could react with the ventricular myosin light chain 1 isolated from different sources, such as human, rat or pig. It was also demonstrated that C8 was directed against the NN part of the N-fragment (amino acid 1-40) of HVMLC1, and both C9 and B12 against the C-fragment (amino acid 99-195).

[The morphologic changes of Amplatzer asymmetric ventricular septal defect occluder after transcatheter closure of perimembrane ventricular septal defect].

Objective: Catheter closure of perimembrane ventricular septal defect (PMVSD) using the Amplatzer asymmetric ventricular septal defect occluder (AAVSDO) is a potential alternative for open surgical repair. However, the profile of the device obtained after closure probably continues to change some concerns regarding its safety. This study was designed to evaluate the morphologic changes of AAVSDO by transthoracic echocardiography examination (TTE) and X-ray examination after transcatheter closure of PMVSD.