Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
March 2004
Objective: To evaluate the replication and encapsidation of HBV mutants with the truncated C gene.
Methods: The HBV mutants with the truncated C gene were constructed by molecular cloning and PCR-based deletion in vitro. The replication and encapsidation of HBV mutants were investigated by Southern blotting, PCR and real-time fluorescence PCR respectively after transfecting the HBV mutants plasmid into HepG2 cells by using liposome.