Casein kinase 1 (CK1) promotes the proliferation and metastasis of glioma cells via the phosphatidylinositol 3 kinase-matrix metalloproteinase 2 (AKT-MMP2) pathway.

Background: Glioma is a type of tumor that usually occurs in the adult central nervous system. Protein kinases have become important targets for oncotherapy since they are closely correlated with signal transduction. The role of the casein kinase 1 (CK1) gene in glioma remains to be fully elucidated.

[The expression and significance of ANXA2 in human glioma].

Aim: to investigate the relation between the expression of ANXA2 in human glioma and its pathological grades, and research the possibility that if ANXA2 could be as a useful diagnostic marker for glioma.

Method: Semi-quantity RT-PCR and immunohistochemistry were proceeded fro corresponding specimens to detect the level of ANXA2 and ANXA2mRNA.

Results: In RT-PCR, the expression level of ANXA2mRNA in glioma was higher than that in surrounding brain tissue.

[Proteomic analysis of rat astrocytes and C6 glioma cells].

Objective: To compare the protein profiles between glioma cells and normal astrocytes.

Methods: Proteomic assay was performed on human C6 glioma cells and purified SD rat astrocytes. Two-dimensional gel electrophoresis (2-DE) and PDQuest software were applied for the comparison of protein image between the C6 cells and astrocytes.

[Expression and significance of ABCG2 in human malignant glioma].

Background & Objective: ATP-binding cassette transporter protein ABCG2 is a marker derived from hematopoietic stem cells. However, its role in tumorigenesis and malignant progression of glioma is unclear. This study was to investigate the expression and significance of ABCG2 in gliomas of different malignant grades.

Expression change of stem cell-derived neural stem/progenitor cell supporting factor gene in injured spinal cord of rats.

Objective: To explore the expression change of stem cell-derived neural stem/progenitor cell supporting factor (SDNSF) gene in the injuried spinal cord tissues of rats, and the relation between the expressions of SDNSF and nestin.

Methods: The spinal cord contusion model of rat was established according to Allen's falling strike method. The expression of SDNSF was studied by RT-PCR and in situ hybridization (ISH), and the expression of nestin was detected by immunochemistry.

Human plasma DNP level after severe brain injury.

Objective: To determine the relationship between DNP level after human severe brain injury and hyponatremia as well as isorrhea.

Methods: The peripheral venous plasma as control was collected from 8 volunteers. The peripheral venous plasma from 14 severe brain injury patients were collected in the 1, 3, 7 days after injury.

[Inhibition of human glioma cell growth by a soluble recombinant human CD40 ligand].

Background & Objective: CD40 presents on B cells, monocytes, dendritic cells, and endothelial cells, as well as a variety of neoplastic cell types, including carcinomas. CD40 stimulated by its antibody or CD40 ligand previously had been demonstrated to induce activation-induced cell death in ovarian carcinoma cell lines, colon carcinoma cell lines, breast carcinoma cell lines, and lung carcinoma cell lines and to inhibit their growth in vivo. This study was designed to assess the effects of a recombinant soluble human CD40 ligand (srhCD40L) on human glioma cell lines.