Adenovirus-mediated Wnt5a expression inhibits the telogen-to-anagen transition of hair follicles in mice.

The canonical Wnt/β-catenin pathway plays an important role in hair cycle induction. Wnt5a is a non-canonical Wnt family member that generally antagonizes canonical Wnt signaling in other systems. In hair follicles, Wnt5a and canonical Wnt are both expressed in cells in the telogen stage.

Adenovirus-mediated Wnt10b overexpression induces hair follicle regeneration.

Hair follicles periodically undergo regeneration. The balance between activators and inhibitors may determine the time required for telogen hair follicles to reenter anagen. We previously reported that Wnt10b (wingless-type mouse mammary tumor virus integration site family member 10b) could promote the growth of hair follicles in vitro.

Immunolocalization of Wnt5a during the hair cycle and its role in hair shaft growth in mice.

Previous studies have shown that the Wnt signaling pathway plays an important role in the growth and development of hair follicles. It has been generally accepted that Wnt5a, a non-canonical Wnt gene, inhibits the Wnt/β-catenin signaling pathway. Several reports have addressed its mRNA expression in embryonic and postnatal hair follicles, but its exact role in the growth of hair follicles is currently unknown.

The construction of siRNA plasmid targeting mouse HIF-1alpha and in vitro study of its inhibition effect.

Objective: To construct effective RNA-interference plasmids targeting mouse HIF-1alpha gene and testify their effects and specificities in interfering HIF-1alpha expression.

Methods: Three RNA-interference plasmids targeting mouse HIF-1alpha gene, pBS/U6/HIF-1alpha-siRNAI~III, were constructed and identified using double digestion method in the present study. RT-PCR, immunostaining and western blotting were employed to detect the expression alterations of HIF-1alpha in 293T cells following transfections of the three plasmids, respectively.