Establishment and evaluation of MIRA-qPCR assay for the rapid and sensitively detection of .

(MP) is a common cause of respiratory infections, and its incidence has increased post-COVID-19 due to "immune debt." Real-time quantitative polymerase chain reaction (qPCR) is the standard for detecting MP, but it has a lengthy detection time. This study aimed to establish a highly sensitive rapid detection method for MP.

The dysfunction of CD4(+)CD25(+) regulatory T cells contributes to the abortion of mice caused by Toxoplasma gondii excreted-secreted antigens in early pregnancy.

Toxoplasma gondii is an opportunistic intracellular parasite that is highly prevalent in human and warm-blooded animals throughout the world, leading to potentially severe congenital infections. Although the abortion caused by T. gondii is believed to be dependent on the timing of maternal infection during pregnancy, the mechanism remains unclear.

[Effects of excreted/secreted antigens of Toxoplasma gondii on CD4+ CD25+ Foxp3+ T cells and NK cells of melanoma-bearing mice].

Objective: To explore the effects of excreted/secreted antigens (ESA) of Toxoplasma gondii on CD4+CD25+ Foxp3+ T cells and NK cells of melanoma-bearing mice, as well as the tumor growth.

Methods: B16F10 (denoted B16) tumor cells were cultured in complete medium and maintained by serial passage in vitro. The 2x 10(5) B16 tumor cells were injected into the right flank of the mouse to establish the tumor-bearing mice model.

[Changes of CD4+CD25+ T cells in the spleen of mice infected with Toxoplasma gondii].

Objective: To observe the changes of CD4+CD25+ regulatory T cells in the spleen of mice infected with T.gondii.

Methods: Twenty-eight female C57BL/6 mice were randomly divided into four groups.