The Correlation Between Detection Value of Distortion-Product Otoacoustic Emissions and the Early Prognosis of Sudden Sensorineural Hearing Loss.

Background: To explore the correlation between the detection value of distortion-product otoacoustic emissions and the early prognosis of sudden sensorineural hearing loss.

Methods: Seventy-eight patients with first-onset sudden sensorineural hearing loss (all frequencies) from April 2018 to July 2019 were included in this study. Distortion-product otoacoustic emissions and pure-tone audiometry tests were performed at days 0, 3, and 6 of admission.

Isolation, characterization and transcriptome analysis of porcine deltacoronavirus strain HNZK-02 from Henan Province, China.

Porcine deltacoronavirus (PDCoV), an emerging porcine enteropathogenic coronavirus, causes acute watery diarrhea and vomiting in piglets. Here, we isolated a strain of PDCoV from intestinal content of a piglet with severe watery diarrhea on a farm located in Henan Province, named PDCoV strain HNZK-02. Subsequently, the complete genomes of cell-cultured PDCoV HNZK-02 passage 5 and 15 were sequenced and analyzed.

[Boost effect of recombinant IL-4 on protection of Schistosoma japonicum cathepsin B DNA vaccine in mice against the parasite].

Objective: To investigate the enhancement effect of IL-4 expression plasmid on cathepsin B DNA vaccine of Schistosoma japonicum (Sj) in mice.

Methods: The recombinant IL-4 plasmid constructed by cloning PCR amplified product of murine IL-4 gene into eukaryotic expression vector pcDNA3.1 was co-injected intramuscularly with Sj cathepsin B expression plasmid DNA to mice as the test group.

Phage displaying peptides mimic schistosoma antigenic epitopes selected by rat natural antibodies and protective immunity induced by their immunization in mice.

Aim: To obtain the short peptides mimic antigenic epitopes selected by rat natural antibodies to schistosomes, and to explore their immunoprotection against schistosomiasis in mice.

Methods: Adults worm antigens (AWA) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked transferred immunoblotting methods with normal SD rat sera (NRS). The killing effects on schistosomula with fresh and heat-inactivated sera from SD rats were observed.

[Cloning and characterization of new genes of Schistosoma japonicum].

Objective: To clone and characterize new genes of Schistosoma japonicum, Sj, and to provide efficient vaccine candidates.

Methods: Sj adult cDNA library was screened with rabbit sera raised against male worm soluble antigen. The inserted cDNA fragments from the positively selected clones were amplified with PCR and further sequenced, as well as characterized through internet NCBI GenBank software.

[Mucosal immunization of recombinant Schistosoma japonicum ferritin].

Objective: To subclone and express the gene encoding Schistosoma japonicum ferritin (SjFer) and study its immune protection against challenge infection in mice vaccinated intranasally.

Methods: The SjFer gene was amplified by PCR, and subcloned into the N-terminal of intein 2 of the pTWIN1 vector. The positive recombinant was screened by PCR, restriction enzyme digestion and sequence analysis.

Schistosoma japonicum: isolation and identification of peptides mimicking ferritin epitopes from phage display library.

In an attempt to isolate and identify the antigenic epitopes on ferritin of Schistosoma japonicum (SjFer) and to test their protective potentiality against Schistosoma japonicum (S.j), polyclonal antisera against SjFer was prepared to screen a 12-mer random peptide library. Three rounds of biopanning were performed and resulted in an enrichment.

[Shistosoma japonicum: dynamics of cytokine production induced by immunization of mice with recombinant GST-Sj32 protein before and after challenge infection].

Aim: To explore the mechanism of protective immunity of rGST-Sj32.

Methods: BALB/c mice were immunized subcutaneously with rGST-Sj32 emulsified with Freund's complete adjuvant. Five mice from each group were killed prior to immunization, prior to challenge and 10 days, 30 days and 45 days post-challenge, respectively.

[Study on the subcloning, expression and immunoprotection with Schistosoma japonicum CAI gene].

Objective: To subclone and express the new gene of Schistosoma japonicum (Sj) CAI and evaluate the immunoprotective effect of the recombinant molecule.

Methods: The cDNA of SjCAI gene was subcloned into expression vector pGEX-5X-3 to form recombinants which were then used to transform to E. coli strain ER 2566.

[Studies on the mimic short peptide-based vaccine: immunoprotection in mice against Schistosoma japonicum infection].

Objective: To evaluate the immunoprotective effect of the mimic short peptide-based vaccine of Schistosoma japonicum (S.j) in mice.

Methods: Phage random peptide library of 12 amino acids was immunoscreened with purified IgG from sera of rats.

[Immunoscreening of Schistosoma japonicum adult worm cDNA library with sera vaccinated with cercaria antigen and analysis of novel genes].

Objective: To explore antigens possessing common immunogenicity with Schistosoma japonicum (Sj) cercariae antigens, and to find out new candidate antigens for schistosomiasis diagnosis and vaccine.

Methods: Sj adult cDNA library was screened using sera from rabbits vaccinated with Sj cercariae antigen, the inserts of positive clones were amplified by PCR, all positive clones were sequenced and the data were analysed using Nucleotide BLAST software of NCBI and Expert Protein Analysis System of Swiss Institute of Bioinformatics.

Results: Thirteen positive clones were obtained after three rounds of immunoscreening, and all amplified by PCR.

[Study on diagnosis of schistosomiasis by ELISA using periodate-treated soluble egg antigen].

Objective: To improve SEA-ELISA, an immunodiagnostic assay for schistosomiasis.

Methods: Soluble egg antigen (SEA) of Schistosoma japonicum was treated with sodium periodate (SP) in order to oxidate its glycosylated epitopes. ELISA using the treated SEA was then performed to detect specific antibodies to SEA in the sera of schistosomiasis patients.

[Studies on fractionation and protective immunity of the membrane antigen from hepato-portal juveniles of Schistosoma japonicum].

Objective: To explore the immunological characteristics of the membrane antigen from hepato-portal juveniles of Schistosoma japonicum and its protective immunity against S. japonicum (Sj) in mice.

Methods: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and enzyme-linked immune electro-transfer blot(EITB) methods were used to recognize the membrane antigens from hepato-portal schistosomula (SjHmAg) by infected rabbit sera (IRS) and normal rabbit sera (NRS).

Molecular cloning, expression and vaccination of a novel gene Sj-MA of Schistosoma japonicum.

In order to obtain new gene and to develop the new vaccine candidate of immunoprotection against Schistosoma japonicum (Sj), Sj adult worm cDNA library was screened with anti-sera to soluble male adult worm antigen and resulted in the discovery of a novel gene designated as Sj-MA. Sequence analysis showed that Sj-MA as a complete cDNA contains one open reading frame. It was deduced to contain 249 amino acid residues and encode a 28.

[Intranasal or intragastric vaccination of mice with recombinant Schistosoma japonicum ferritin induces immune protection against challenge infection].

Objective: To test the immune protection against challenge infection in mice vaccinated intranasally or intragastrically with recombinant Schistosoma japonicum (S.j) Ferritin (rSjFer).

Methods: Mice were divided into 8 groups each with 10 mice.

[Protective effect against Schistosoma japonicum of recombinant fusion protein SjGST-32 in mice].

Objective: To explore the optimal immune doses of recombinant antigen rSjGST-32, with QuilA as adjuvant.

Methods: BALB/c mice were immunized with doses of 50, 100 and 200 micrograms rSjGST-32/mouse plus 50 micrograms QuilA adjuvant. QuilA and PBS were used as controls.

Cloning, expression and immunization of the new antigen gene Sj-Ts4 of Schistosoma japonicum.

In order to explore the molecular mechanism of high immune protection against schistosomes infection in animals infected with Trichinella spiralis, and to provide several cross-protective antigen genes for developing anti-schistosomiasis vaccine, a Schistosoma japonicum adult worm cDNA library was immunoscreened using sera taken from mice infected with Trichinella spiralis. Nine positive clones were obtained after 3 rounds of immunoscreening. Among them, Sj-Ts4 represents a novel gene of S.