Circ_0000144 facilitates the progression of thyroid cancer via the miR-217/AKT3 pathway.

Background: Thyroid carcinoma (TC) is the most common malignancy of the endocrine system. Circular RNA (circRNA) is vital in the regulation of tumor progression. Circ_0000144 serves as a novel oncogenic circRNA, and miR-217 is reported to inhibit the malignant phenotypes of cancer cells by targeting AKT3 in TC.

Cell penetrating peptide of sodium-iodide symporter effect on the I-131 radiotherapy on thyroid cancer.

The aim of the present study was to clarify whether the cell penetrating peptide of sodium-iodide symporter (NIS) has an effect on the I-131 radiotherapy of thyroid cancer. Firstly, we combined the HIV-1 TAT peptide (a cell penetrating peptide, dTAT) and established a nanoparticle vector (dTAT NP) to study the delivery efficiency of this cell-penetrating strategy for tumor-targeted gene delivery. dTAT NP was transfected into cultured TPC-1 cells as a model to study the effects of I-131 radiotherapy on thyroid cancer.

[In vivo and in vitro stability of ¹³¹I-Herceptin and its form of existence in the blood of rabbits].

Objective: To evaluate the in vivo and in vitro stability of (131)I-Herceptin and its form of existence in the blood.

Methods: Herceptin was labelled with iodine-131 using the Iodogen method. (131)I-Herceptin was stored at 4 degrees celsius for 3, 24, 48, 72 and 96 h, and the radiochemical purity (RCP) was measured by high performance liquid chromatography (HPLC).

[Mechanism of cardiotoxicity associated with Herceptin using (131)I-Herceptin radioimmunoimaging].

Objective: To study the mechanism of cardiotoxicity associated with Herceptin.

Methods: Herceptin was labeled with iodine-131 using the Iodogen method. Radioimmunoimaging was performed in 5 rabbits at 3 h to 5 days following (131)I-Herceptin injection to investigate the biodistribution of Herceptin.

[Relationship between fluorodeoxyglucose uptake and vascular endothelial growth factor in early-stage nasophygeal carcinoma].

Objective: To study the overexpression of vascular endothelial growth factor (VEGF) and fluorine-18 fluorodeoxyglucose (FDG) uptake in early-stage nasopharyngeal carcinoma (NPC) and evaluate their relationship.

Methods: FDG positron emission tomography (PET) was performed in forty patients with stage I and stage II NPC. The maximum and mean standard uptake values (SUVmax and SUVmean, respectively) were measured in each patient, and the expression of VEGF was measured on paraffin sections using immunohistochemistry.

[Analysis of radioactive distribution in the sacrum in whole-body bone scanning].

Objective: To analyze the radiogenic distribution in the sacrum in whole-body bone scanning.

Methods: A total of 212 patients receiving whole-body bone scanning without any explicit bone metastases were divided into different age and gender groups. The radioactive distribution in the sacrum in whole-body bone scanning was analyzed statistically.

[Effects of interferon-gamma on Her-2/neu expression and antitumor activity of 131I-Herceptin in breast cancer cell lines].

Background & Objective: Herceptin plays an important role in treating metastatic breast cancer by targeting Her2/neu, therefore, combining Herceptin with iodine-131 (131I) might enhance its antitumor activity. This study was to up-regulate Her2/neu expression by interferon-gamma (IFN-gamma), and explore its effect on binding and antitumor activity of 131I-Herceptin in breast cancer cell lines MCF-7, SKBR-3 and BT-474.

Methods: MCF-7, SKBR-3 and BT-474 cells were cultured with or without IFN-gamma (500 U/ml) for 48 h.

[Preparation, quality control and biodistribution of 131I-herceptin in New Zealand rabbits].

Objective: To study the immunoactivity,biodistribution and metabolic pattern of (131)I-Herceptin in rabbits.

Methods: Herceptin was radiolabelled with (131)I and its radiochemicalpurity (RCP) measured by size-exclusion high-pressure liquid chromatography (HPLC). The binding rate to BT-474 cells was measured to evaluate the immunoactivity of (131)I-Herceptin.

[Killing effect of sequential Herceptin and adriamycin treatment on breast cancer cell line in vitro].

Objective: To observe the killing effect of Herceptin and adriamycin sequentially applied on breast cancer cell line in vitro.

Methods: BT-474 human breast cancer cells in exponential growth phase were treated with Herceptin alone, adriamycin alone and their sequential administration (Herceptin before adriamycin and vice versa), respectively. Under optical microscope, the morphological changes of the cells were observed before and after drug administration.

[Therapeutic effects of strontium-89 against osseous metastases of lung cancer: analysis of 126 cases].

Objective: To investigate the therapeutic effects of strontium-89 against osseous metastases of lung cancer.

Methods: A total of 126 patients with osseous metastases of lung cancer received strontium-89 treatment ((89)SrCl(2)) at the dose of 148 MBq given through a single intravenous injection. The analgesic effect was evaluated by the changes in the degree, frequency and scores of the pain, and the therapeutic effect assessed by observing the changes in the number and volume of osseous lesions after therapy and compared between different pathological types of lung cancer by Ka-square test.

[Diagnostic value of 99mTc-MIBI brain SPECT for brain glioma].

Objective: To evaluate the value of (99m)Tc-MIBI brain single-photon emission computerized tomography (SPECT) in diagnosis of glioma.

Methods: Fifty-nine patients with glioma, 6 with brain abscess and 9 healthy controls underwent (99m)Tc-MIBI brain SPECT, and the diagnostic indices such as the sensitivity, specificity and accuracy were calculated. The tumor to non-tumor (T/NT) ratios were calculated according to the region of interest (ROI) and compared between the glioma group, healthy control group and brain abscess group by t test.

[Diagnosis of osseous metastases of malignant tumors by bone scanning combined with bone alkaline phosphatase detection].

Objective: To assess the diagnostic value of bone alkaline phosphatase (B-AKP) detection in diagnosis of osseous metastases of malignant tumors.

Methods: Bone scanning and B-AKP detection were performed in 106 patients with malignancies. According to the findings in bone imaging and clinical symptoms, the patients were divided into bone metastases group (BM) and non-bone metastases group (NBM), between whom B-AKP was compared by t test.

[Lower limb hemodynamic evaluation in patients with diabetic foot using 99mTc-methylene diphosphonate three-phase bone scintigraphy].

Objective: To evaluate the efficacy of (99m)Tc-methylene diphosphonate (MDP) bone scintigraphy in diagnosis of diabetic foot.

Methods: (99m)Tc-MDP three-phase bone imaging was performed in 56 patients with diabetic foot and 36 non-diabetic control subjects, each foot was analyzed independently. The radioactive concentration per pixel hence the foot to tibia ratio (R(m/t)) was calculated and the time-radioactivity curve of each region of interest generated.

[Evaluation for degree of malignancy and prognosis of patients with brain glioma using 99Tcm-MIBI brain SPECT].

Background & Objective: It is important and useful to evaluate the degree of malignancy for therapeutic scheme and prognosis evaluation. Traditional imaging methods such as CT and MRI play the important role in distinguishing glioma, but sometimes it was still difficult to diagnose accurately only having the aid of these anatomic information. Stereotaxic needle biopsy is an invasive method and indicates only local pathologic change, sometimes the error of sample collection will result in inaccurate grading.

[Repair of tibial defect with tissue-engineered bone graft and radionuclide bone imaging in goats].

Objective: To observe the effect of tissue-engineered bone grafts in repairing large tibial defect in goats, and assess the value of radionuclide bone imaging in monitoring the therapeutic effect of this approach.

Methods: Tibial defects measuring 2 cm was artificially made in the left tibia of 27 normal goats that were subsequently divided into 3 groups (9 each) to undergo treatment with tissue-engineered bone grafts, artificial bone grafts or without any grafts (as control group) respectively. The tissue-engineered bone grafts contained bone marrow stroma cells (BMSCs) of the goats and coral hydroxyapatite (CHAP), while the artificial bone grafts were from CHAP only.

Radiosensitivity of hepatocarcinoma cell line BEL-7402.

OBJECTIVE: To assess the radiosensitivity of human hepatocarcinoma cell line BEL-7402. METHODS: Human hepatocarcinoma cell line BEL-7402 was cultured in vitro and then subjected to exposure to 6 MV X-ray at the doses ranging from 0 to 10 Gy. The survival rate of the cells following the exposure was assessed by determining the colony-forming units during further cell culture 12 to 14 d after the exposure, and the parameters for the radiosensitivity calculated.