Publications by authors named "Yi-Wan Tan"
Successful intrauterine hematopoietic cell transplantation (IUT) for congenital hemoglobinopathies is hampered by maternal alloresponsiveness. We investigate these interactions in semi-allogenic murine IUT. E14 fetuses (B6 females × BALB/c males) were each treated with 5E+6 maternal (B6) or paternal (BALB/c) bone marrow cells and serially monitored for chimerism (>1% engraftment), trafficked maternal immune cells, and immune responsiveness to donor cells.
View Article and Find Full Text PDFIntroduction: Significant limitations with existing treatments for major haemoglobinopathies motivate the development of effective intrauterine therapy. We assessed the feasibility of fetoscopic and ultrasound-guided intrauterine haemopoietic cell transplantation (IUHCT) in macaque fetuses in early gestation when haemopoietic and immunological ontogeny is anticipated to enable long-term donor cell engraftment.
Material And Methods: Fluorescent-labelled bone marrow-derived mononuclear cells from 10 pregnant Macaca fascicularis were injected into their fetuses at E71-114 (18.
Adeno-associated viral vectors (AAVs) achieve stable therapeutic expression without long-term toxicity in adults with hemophilia. To avert irreversible complications in congenital disorders producing early pathogenesis, safety and efficacy of AAV-intrauterine gene transfer (IUGT) requires assessment. We therefore performed IUGT of AAV5 or -8 with liver-specific promoter-1 encoding either human coagulation factors IX (hFIX) or X (hFX) into Macaca fascicularis fetuses at ∼0.
View Article and Find Full Text PDFArticle Synopsis
- Major hemoglobinopathies, like thalassemia, can put a significant strain on healthcare systems, prompting the exploration of advanced treatment methods like intrauterine hemopoietic cell transplantation (IUHCT) and gene transfer (IUGT) to improve outcomes over traditional postnatal therapies.
- Research using thalassemic HbbTh3/+ mice showed that IUHCT with co-isogenic cells and high-dose bone marrow cells could create different levels of chimerism, resulting in better health indicators and smaller spleens compared to untreated mice.
- Although IUGT with a lentiviral vector improved some hematological measures, further optimization of doses is needed to enhance efficacy, particularly when combining IUHCT with post
Article Synopsis
- Managing complicated monochorionic twins and fetal anomalies is challenging in areas without advanced fetal therapy, prompting efforts in rapid selective feticide methods like radiofrequency ablation (RFA) and selective fetoscopic laser photocoagulation (SFLP) to treat twin-to-twin transfusion syndrome (TTTS).
- In a study involving five pregnancies, RFA techniques were quickly learned, while SFLP required a more structured learning approach under the guidance of an experienced proctor, leading to successful live births for most co-twins.
- The experience shows that supervised directed learning can help smaller medical units quickly provide effective fetal therapy services, complementing traditional training methods and enhancing overall team capabilities.