Systematic research of Hdedpa derivatives as potent inhibitors of New Delhi Metallo-β-lactamase-1.

New Delhi Metallo-β-lactamase-1 (NDM-1), a Zn (II)-dependent enzyme, can catalyze the hydrolysis of almost all β-lactam antibiotics including carbapenems, resulting in bacterial antibiotic resistance, which threatens public health globally. Based on our finding that Hdedpa is as an efficient NDM-1 inhibitor, a series of Hdedpa derivatives was systematically prepared. These compounds exhibited significant activity against NDM-1, with IC values 0.

Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa.

To investigate whether parallel complementary RNA (pRNA) could induce gene-specific silencing in Pseudomonas aeruginosa, pRNA of the mexA gene was expressed in it. Compared to the control strains, the strain expressing pRNA of mexA showed a 50% decrease in minimum inhibitory concentrations (MICs) of several antimicrobial agents and a twofold increase in the initial accumulation rate of ethidium bromide, all of which are substrates of the MexAB-OprM efflux pump. These results suggest that gene-specific silencing was induced by pRNA.