Protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment against Pneumocystis carinii in mice.

Objective: To evaluate the protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment of Pneumocystis carinii (P. carinii) against P. carinii in mice.

[Immunogenicity of p55 gene fragment from Pneumocystis carinii].

Objective: To evaluate immunogenicity of the recombinant protein GST-p55/570 of Pneumocystis carinii.

Methods: The fusion protein GST-p55/570 was expressed from the prokaryotic expression plasmid pGEX-570, and purified by using glutathione-agarose. The expressed product was analyzed by SDS-PAGE.

[Construction and expression of prokaryotic expression plasmids of Pneumocystis carinii p55 antigen gene fragment].

Objective: To construct prokaryotic recombinant expression plasmid carrying Pneumocystis carinii Mr 55 000 antigen (p55) gene fragment and express the recombinant protein.

Methods: P. carinii pneumonia (PcP) rat models were established by subcutaneous injection of dexamethasone for 14 weeks.