The necroptosis signature and molecular mechanism of lung squamous cell carcinoma.

Background: Given the poor prognosis of lung squamous cell carcinoma (LUSC), the aim of this study was to screen for new prognostic biomarkers.

Methods: The TGCA_LUSC dataset was used as the training set, and GSE73403 was used as the validation set. The genes involved in necroptosis-related pathways were acquired from the KEGG database, and the differential genes between the LUSC and normal samples were identified using the GSEA.

Complexation with pagoda[]arene ( = 4, 5) protects ferrocenium from oxidation.

The complexation behaviors of pagoda[]arenes ( = 4, 5) with ferrocene, ferrocenium and analogues cobaltocenium were studied. The inclusion complexes of pagoda[]arenes can protect ferrocenium from oxidation in organic solvents and improve the stability and oxygen resistance of ferrocenium.

Pagoda[]arenes ( = 4, 5): Extremely Strong Binders for the Tropylium Cation.

Herein, host-guest complexation between pagoda[]arenes ( = 4, ; = 5, ) and tropylium cation () was investigated in detail. It was found that both and showed surprisingly strong binding affinities toward the tropylium cation with association constants of more than 10 M for the 1:1 host-guest complexes. The theoretical calculations showed different host-guest complexion ways for complexes @ and @ and the strong π···π interactions and multiple C-H···π interactions play a very important role in the formation of these stable complexes, respectively.

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma.

Background: Spindle and kinetochore-associated complex subunits 1-3 (SKA1-3) stabilize the kinetochore-attached spindle microtubules in metaphase. Due to the dysregulation in multiple cancers, SKA1-3 is considered a predictor for the prognosis of the patients. However, the potential clinical applications of SKA1-3, particularly in hepatocellular carcinoma (HCC) prognosis and progression, have completely unknown yet.

Perylenequinone-based "turn on" fluorescent probe for hydrogen sulfide with high sensitivity in living cells.

Hydrogen sulfide (HS) is a kind of gaseous signal molecule in many physiological processes. In order to detect HS, a novel "turn on" fluorescent probe 6,12-dihydroxyperylene-1,7-dione (DPD) was designed and synthesized. The probe DPD is fluorescence silence, while the addition of HS induces an obvious green fluorescence with an obvious color change from dark blue to yellow-green.

Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture.

Gecko () extracts have been used in traditional Chinese medicine for many years. It has been proven that the gecko polypeptide mixture (GPM) extracted from gecko can inhibit the growth of multiple types of tumor cells. In order to investigate the possible anti-tumor molecular mechanisms of GPM, we used RNA-seq technology to identify the differentially expressed genes (DEGs) of human hepatocellular carcinoma (HCC) HepG2 cells treated with or without GPM.