Publications by authors named "Yi-Long Zhao"
Objective: To investigate the expression of Zfx gene in spermatogenic cells.
Methods: The testes of d6, d8, d17 and adult mice were collected, single cell suspension was prepared by combinatorial enzyme digestion, spermatogenic cells were isolated by BSA density gradient method, and Zfx expression was detected by real-time quantitative polymerase chain reaction (qRT-PCR) and Western Blot (WB).
Results: Single cell suspension prepared by combination enzyme digestion method and density gradient method laid with BSA can obtain various types of spermatogenic cells with purity>85%; The expression level of the Zfx gene is low in primitive type A spermatogonia, type A spermatogonia, and type B spermatogonia, whereas it is high in preleptotene spermatocytes, pachytene spermatocytes, and round spermatid cells.
Article Synopsis
- Low to moderate ethanol intake may protect neurons from damage caused by ischemia/reperfusion, but the exact mechanism is still unclear.
- This study found that low-dose ethanol increased neuron survival during oxygen-glucose deprivation and reoxygenation, while reducing harmful calcium levels and apoptosis.
- The protective effects were linked to the activation of a specific potassium channel (BK channel) and changes in the expression of apoptosis-related proteins Bcl-2 and Bax.