Evidence that exogenous urea acts as a potent cue to alleviate ammonium-inhibition of root system growth of cotton plant (Gossypium hirsutum).

Many plants grown with low-millimolar concentration of NH as a sole nitrogen source develop NH -toxicity symptoms. To date, crucial molecular identities and a practical approach involved in the improvement of plant NH -tolerance remain largely unknown. By phenotyping of upland cotton grown on varied nitrogen forms, we came across a phenomenon that caused sub-millimolar concentrations of urea (e.

[Effect of polysaccharides content of tissue culturing seedlings on Dendrobium candidium under sound wave stimulation].

Objective: To detect the polysaccharides content of tissue culturing seedlings on Dendrobium candidium under special sound wave stimulation.

Methods: The content of polysaccharides was detected by phenol-vitriol colorimetry method.

Results: The polysaccharides content of the groups stimulated continuously for 24d was higher obviously than the content of the control groups.

[A comparative study on isoenzymes of Sarcocystis spp. from cattle and water buffaloes].

Objective: To analyze the zymogram of peroxidase (PER) and phosphoglucose isomerase (PGI) of three species of Sarcocystis.

Methods: The collected parasites were homogenized and fragmented by ultrasonication. After centrifugation, the supernatants were analyzed by isoelectric focusing electrophoresis.

Characterization of the human PAP1 gene and its homologue possible involvement in mouse embryonic development.

We have identified PAP1 gene, a novel member of the immunoglobulin superfamily (IGSF) from U251-pTet-p53 cell line, which carried a wild-type p53 transgene. The gene has been localised to chromosome 16p12-13. Alignment of the predicted protein sequence for Human, Pan troglodytes, Canis, Mus musculus and Gallus gallus revealed it was highly conserved.

Effects of exogenous p53 transfection on the gene expression in the human brain glioma cell line U251.

The p53 gene is activated in response to several malignancy-associated stress signals by transactivation of downstream genes and by transcription-independent mechanisms. In order to identify new p53 downstream genes, we established a new system of p53 gene inducible expression, U251-pTet-p53 cell line, with the Tet-On Gene Expression System, in which exogenous p53 gene could overexpress in doxycycline (Dox) medium but not in the medium without Dox. By comparing their random primer RT-PCR products, it was proved that exogenous p53 gene expression could lead to many genes differential expression, some up-expressed and others down-expressed.