[Fret-based single-molecule probes for monitoring induced activation of Rac, Cdc42 signaling pathways in living cells].

Rho GTPases, including Rac1, Cdc42, play a critical role in the regulation of a variety of cellular processes such as cell morphology, cell migration, transcriptional activation and gene expression. We constructed several FRET-based single-molecule probes containing red fluorescent protein dsRed1, cyan fluorescent protein ECFP, the GTPase binding domain of the effector, Pak1 or N-WASP, and Rac1 or Cdc42. Rac1 and Cdc42 signaling pathways were activated in transfected cells by the inducer, insulin or bradykinin respectively.

[Cytological observation of anther abortion and starch distribution of a cytoplasm male sterile pepper (Capsicum annum L.)].

A cytoplasm male sterile pepper (Capsicum annum L.) was examined using cytochemical method to study its pollen abortion. Thick sections of both anthers of male sterility line 8214A and its maintainer 8214B at different stages were stained using Periodic Acid-Schiff's (PAS) reaction to detect starch distribution.

[Calcium distribution in the central cell of lettuce (Lactuca sativa L.) before and after pollination].

Potassium antimonite precipitation was used to locate calcium in the central cell of lettuce (Lactuca sativa L.) before and after pollination. At 3d before anthesis, two polar nuclei of central cell separately located at two polarity of the cell, and few calcium precipitates (ppts) appeared in the polar nuclei and cytoplasm, but some ppts in its small vacuoles.

[Studies on the calcium distribution in developing synergids of lettuce (Lactuca sativa L.)].

Potassium antimonite was used to locate calcium in the synergids of lettuce (Lactuca sativa L) during their development. The two synergids on 3d before anthesis formed evident polarity with most cytoplasm located in the micropylar end and nucleus in the middle and a big vacuole in the chalazal end. At this time, calcium precipitates were a few in both cells.

[Calcium distribution in the egg cell, zygote and proembryo of lettuce (Lactuca sativa L.)].

Potassium antimonite precipitation was used to located calcium in the egg cells (before and after anthesis), zygotes and proembryos of lettuce (Lactuca sativa L.). A few calcium precipitates (ppts) were located in the small vacuoles of cytoplasm of egg cell at 3 d before anthesis, when egg cells just formed.

[The character of calcium distribution in developing anther of lettuce (Lactuca sativa L.)].

Potassium antimonite was used to locate calcium in the anther of lettuce (Lactuca sativa L) during its development. At the early stage of anther development there were few calcium granules in microspore mother cells and the cells of anther wall. After meiosis of microspore mother cells, calcium granules first appeared in the tapetal cells in which some small secretive vacuoles containing many calcium granules were formed and secreted into locule.

[Calcium distribution in fertile and sterile anthers of a genic male sterile Chinese cabbage].

Potassium antimonite was used to locate calcium in the fertile and sterile anthers of a genic male sterile Chinese cabbage (Brassica campestris L. ssp. chinensis Makino) to probe the relation between Ca(2+) and fertility and sterility of anthers of the cabbage.

[The dynamics of calcium distribution in stigma and style of lettuce (Lactuca sativa L.) before and after pollination].

Potassium antimonite was used to deposit calcium in the stigma and style of lettuce (Lactuca sativa L.) before and after pollination. The stigma of lettuce is two splits.

[Expression of the recombinant SARS coronavirus nucleocapsid protein in Pichia pastoris and identification of its bioactivity].

The full length cDNA of SARS coronavirus nucleocapsid (N) protein was amplified by PCR and cloned into yeast expression vector pPIC3.5K to generate expression vector pPIC3.5K-SCoVN.

[The dynamic of calcium distribution during megasporegenesis of lettuce (Lactuca sativa L.)].

Potassium antimonite was used to deposit calcium in the young ovule of lettuce (Lactuca sativa L.) at megasporogenesis stage to study the relationship between calcium and megaspore degeneration. At the megaspore mother cell stage, few calcium granules were formed in the cell (Plate I-1, 2).

[The distribution of calcium in the stigma and style of tobacco during pollen germination and tube growth].

After pollen grains of tobacco landed on stigma they begin to hydrate and form many small vesicles containing some calcium grains in cytoplasm. The calcium stored in pollen wall is released into tectum of stigma to make a calcium-rich environment. When a pollen tube penetrates the tectum and grows between stigma cells, numerous calcium precipitates appear in the tip tube wall.