Determination of quinolones residues in prawn using high-performance liquid chromatography with Ce(IV)-Ru(bpy)(3)2+-HNO3 chemiluminescence detection.

A novel method was developed for the determination of quinolone (QN) residues such as ofloxacin, norfloxacin, ciprofloxacin and lomefloxacin by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The procedure was based on the chemiluminescent enhancement by QNs of the Ce(SO(4))(2)-Ru(bpy)(3)(2+)-HNO(3) system. The separation was carried out with an isocratic elution using the mobile phase of 3:15:82 (v/v/v) acetonitrile-methanol-ammonium acetate buffer (containing 7.