Protein tyrosine phosphatase 1 protects human pancreatic cancer from erastin-induced ferroptosis.

Background: Pancreatic ductal adenocarcinoma (PDAC) is a fatal malignancy due to the lack of early detection method, therapeutic drug and target. We noticed that the expression of Protein Tyrosine Phosphatase Mitochondria1(PTPMT1) is upregulated in PDAC. However, its role in pancreatic cancer remains unknown.

Synthesizing High--Value Diffusion-weighted Imaging of the Prostate Using Generative Adversarial Networks.

Purpose: To develop and evaluate a diffusion-weighted imaging (DWI) deep learning framework based on the generative adversarial network (GAN) to generate synthetic high--value ( =1500 sec/mm) DWI (SYN) sets from acquired standard--value ( = 800 sec/mm) DWI (ACQ) and acquired standard--value ( = 1000 sec/mm) DWI (ACQ) sets.

Materials And Methods: This retrospective multicenter study included 395 patients who underwent prostate multiparametric MRI. This cohort was split into internal training (96 patients) and external testing (299 patients) datasets.

Novel role of Snail 1 in promoting tumor neoangiogenesis.

Snail1 plays an important role in epithelial to mesenchymal transition (EMT) during tumor metastasis; however, whether Snai1 potentiates the process of neoangiogenesis is completely unknown. In the present study, tube formation assay was used to evaluate neoangiogenesis The expression of Snai1 and other pro-neoangiogenic factors was measured by quantitative real time PCR. Tumor derived endothelial cells (TDECs) were stimulated with fibroblast growth factor 1 (FGF1) or VEGF and formed more tubes compared with untreated, whereas cells treated with Sulforaphane had less tube formation.

[Construction of Lentiviral Vector Over-Expressing MEG3 and Its Effect on XG-7 Cell Apoptosis].

Objective: To construct a recombinant lentiviral expression vectors carrying MEG3 and to evaluate its effects on XG-7 cell apoptosis.

Methods: A full-length genomic fragment of human MEG3 was cloned from the pcDNA3.0-MEG3 packaging plasmid and was amplified by PCR.

SIRT1 mediates Sphk1/S1P-induced proliferation and migration of endothelial cells.

Angiogenesis is one of the most important components of embryonic organ formation and vessel growth after birth. Sphingosine kinase 1 (Sphk1) and S1P has been confirmed to participate in various cell signaling pathways and physiological processes including neovascularisation. However, the mechanisms that Sphk1/S1P regulates neovascularisation remain unclear.

Hepatocyte growth factor enhances the inflammation-alleviating effect of umbilical cord-derived mesenchymal stromal cells in a bronchiolitis obliterans model.

Background Aims: Specific and effective therapy for prevention or reversal of bronchiolitis obliterans (BO) is lacking. In this study, we evaluated the therapeutic effect of hepatocyte growth factor (HGF) gene modified mesenchymal stromal cells (MSCs) on BO.

Methods: A mouse model of experimental BO was established by subcutaneously transplanting the tracheas from C57BL/6 mice into Balb/C recipients, which were then administered saline, Ad-HGF-modified human umbilical cord-MSCs (MSCs-HGF) or Ad-Null-modified MSCs (MSCs-Null).

HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity.

Background: Effective therapeutic strategies to address intestinal complications after radiation exposure are currently lacking. Mesenchymal stem cells (MSCs), which display the ability to repair the injured intestine, have been considered as delivery vehicles for repair genes. In this study, we evaluated the therapeutic effect of hepatocyte growth factor (HGF)-gene-modified MSCs on radiation-induced intestinal injury (RIII).

[Effect of anxin granules combined with tirofiba on patients with acute myocardial infarction after elective percutaneous coronary intervention].

To investigate the influence of Anxin granules combined with tirofiban on acute myocardial infarction (AMI) Patients after elective percutaneous coronary intervention (PCI). One hundred and twenty AMI patients were randomly divided into treatment group and control group. The patients in the two groups were all given Tirofiban 30mins before PCI .

[The Mie scattering lidar return signal denoising research based on EMD-DISPO].

Lidar echo signal is a typical non-steady-state, non-stationary signal, and difficult to be dealt with by the traditional filtering methods. As a new signal processing theory proposed in recent years, empirical mode decomposition method can adaptively divide the lidar echo signal into different intrinsic mode function (IMF) components according to different time scale, and noise mainly concentrates in the high-frequency component. However, when filtered with simply removing high frequency component, the useful signal will be possibly reduced.

Overexpression of microRNA-29b induces apoptosis of multiple myeloma cells through down regulating Mcl-1.

MicroRNAs (miRNAs) are small, noncoding ribonucleic acids (ncRNAs), which regulate gene expression by targeting mRNAs for translational repression and degradation. Several lines of evidences have indicated that miRNAs act as tumor suppressors and oncogenes. However, the role of miRNAs in pathogenesis of multiple myeloma (MM) remains unclear.

[Development of the human/rat chimera model with neonatal rats].

The purpose of this study was to transplant neonatal rat with human cord blood Lin(-) cells to test the possibility of this xenograft model. The Lin(-) cells were purified from human cord blood (CB) using negative selection strategy based on different lineage-specific antigens. The Lin(-) cells were injected into the liver of neonatal rats using a microinjector at an average of 5 x 10(5) cells for each.