In Vitro Plasma Protein Binding Determination of Lefamulin by Equilibrium Dialysis and High-Performance Liquid Chromatography Coupled With Evaporative Light Scattering Detector.

A credible method utilizing equilibrium dialysis and high-performance liquid chromatography with an evaporative light scattering detector (HPLC-ELSD) was developed to determine the plasma protein binding of lefamulin in swine. The method demonstrated excellent linearity, with a logarithmic calibration curve in the range of 0.1 to 2 μg/mL (r > 0.