Screening of monoamine oxidase B inhibitors in Tibetan strawberry by ligand fishing based on enzyme functionalized cellulose filter paper.

Tibetan strawberry () is a wild medicinal and edible plant in Tibet possessing various health benefits such as neuroprotection and anti-oxidation. However, there has been little study reported on its chemical constituents. To investigate the inhibitors of monoamine oxidase B (MAO-B) in Tibetan strawberry, we immobilized the enzyme onto cellulose filter paper for the first time to develop a new screening method.

Polyphenolic glycosides with unusual four-membered ring possessing anti-Parkinson's disease potential from black wolfberry.

This work reports the isolation of seven undescribed polyphenolic glycosides (1-7) together with fourteen known compounds (8-21) from the fruit of Lycium ruthenicum Murray. The structures of the undescribed compounds were identified based on comprehensive spectroscopic methods including IR, HRESIMS, NMR and ECD, and chemical hydrolysis. Compounds 1-3 possess an unusual four-membered ring, while 11-15 were firstly isolated from this fruit.

Phloroglucinol derivatives with glucosidase inhibitory activities from .

Four new phloroglucinol derivatives (-) were isolated from the leaves of . Their structures were elucidated by means of extensive spectroscopic data. Among them, compounds and showed significant inhibitory activity against -glucosidase with IC values of 10.

Screening of Monoamine Oxidase B Inhibitors from by Ligand Fishing and Their Neuroprotective Effects.

, known as Tibetan strawberry, is an edible plant possessing various health-promoting effects. However, its functional compositions were rarely studied. In this work, monoamine oxidase B (MAO-B) inhibitors in this plant were rapidly screened using the enzyme-functionalized magnetic nanoparticles coupled with UPLC-QTOF-MS.

Ligand fishing of monoamine oxidase B inhibitors from Platycodon grandiflorus (Jacq.) A.DC. roots by the enzyme functionalised magnetic nanoparticles.

Introduction: As a famous traditional Chinese medicine, roots of Platycodon grandiflorus (Jacq.) A.DC.

Polyphenolic Glycosides from the Fruits Extract of Murr and Their Monoamine Oxidase B Inhibitory and Neuroprotective Activities.

The fruits of Murr have long been consumed as health food and used in folk medicine in China. Apart from the well-known polysaccharides, the active small molecular constituents in this fruit have not been fully studied. In this work, a systematic phytochemical study was carried out to investigate the small molecules and their potential health benefits.

Ligand fishing based on tubular microchannel modified with monoamine oxidase B for screening of the enzyme's inhibitors from Crocus sativus and Edgeworthia gardneri.

A novel strategy of performing ligand fishing with enzyme-modified open tubular microchannel was proposed for screening bioactive components present in medicinal plants. Monoamine oxidase B was immobilized onto the surface of the microchannel for the first time to specifically extract its ligands when the plant's extracts solution flows through the channel. The thermal and the storage stability of immobilized monoamine oxidase B were significantly enhanced after immobilization.

Fishing of α-Glucosidase's Ligands from by α-Glucosidase Functionalized Magnetic Nanoparticles.

α-Glucosidase was immobilized on magnetic nanoparticles (MNPs) for selective solid-phase extraction of the enzyme's ligands present in , which is a medicinal plant used for the treatment of various diseases and possesses anti-diabetic activity. One new compound, aloeacone (), together with two known compounds, aloenin aglycone () and aloin A (), were fished out as the enzyme's ligands. The structure of was determined by HR-MS and comprehensive NMR techniques.

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots.

Dopamine (DA) is a critical biomarker for a variety of neurological diseases. Methods for simple and rapid DA detection are crucial for clinical diagnosis and treatments for those diseases. In this work, we developed a novel pretreatment-free method for dopamine detection using carbon dots as a turn-on fluorescent probe synthesized in situ.

Resorcinol derivatives with -glucosidase inhibitory activities from .

Nine resorcinol derivatives including two new ones, 5-[(8,11,14)-nonadeca-8,11,14-trienyl] resorcinol () and 5-[(8,11,14)-heptadeca-8,11,14-trienyl] resorcinol (), were isolated from the leaves of . The new structures were elucidated by means of extensive spectroscopic techniques including interpretation of 1D and 2D NMR spectra. Among them, compounds , , and exhibited significant -glucosidase inhibitory activities with IC of 3.

New phloroglucinol derivatives with protein tyrosine phosphatase 1B (PTP1B) inhibitory activities from Syzygium austroyunnanense.

Five new phloroglucinol derivatives (1-5) together with one known analogue (6) were isolated from the leaves of Syzygium austroyunnanense which is an edible folk medicine used for the treatment of diabetes. The new structures were elucidated as austroyunones A - E (1-5) by means of the extensive spectroscopic analyses including high-resolution electrospray ionization mass spectrometry (HRESIMS) and 1D and 2D nuclear magnetic resonance (NMR). Compounds 4-6 showed obvious protein tyrosine phosphatase 1B (PTP1B) inhibitory activity.