Titania nanotubes decorated with gold nanoparticles for electrochemiluminescent biosensing of glycosylated hemoglobin.

A glycated hemoglobin (HbA1c) biosensor with high performance has been constructed in this work. Here the fructosyl amino acid oxidase was immobilized onto a pre-functionalized indium tin oxide glass with titania nanotubes decorated with gold nanoparticles. The property of nanocomposite was characterized by transmission electromicroscopy, scanning electron microscopy, electrochemistry and spectroscopy.

Study on a luminol-based electrochemiluminescent sensor for label-free DNA sensing.

Automatic, inexpensive, simple and sensitive methods for DNA sensing and quantification are highly desirable for biomedical research. The rapid development of both the fundamentals and applications of electrochemiluminescence (ECL) over the past years has demonstrated its potential for analytical and bio-analytical chemistry. This paper reports the quenching effect of DNA on the ECL of luminol and the further development of a DNA sensing device.

[Studies on sensitization for electrochemiluminescence of luminol with platinum-gold bimetallic nanoparticles modified electrode].

The present paper reports the researches on sensitization of nano-bimetalic material for the electrochemiluminescence (ECL) of luminol. The platinum-gold bimetallic nanoparticles with different component ratio and size were prepared by chemical reduction. The analytical methods such as UV-Vis spectra, TEM and XRD were applied to characterize the properties ofthe nanoparticles.

[Spectrophotometric determination of deoxyribonucleic acid by its quenching effect on acridine orange].

Based on the reaction of acridine orange with deoxyribonucleic acid (DNA), and under the optimized condition, a novel method to determine the DNA was developed with the spectrophotometry. The absorbance of acridine orange at the maximum absorption wavelength of 444 nm responded to the concentration of DNA negatively with excellent linearity. It has an upper linear limit concentration of 8.

[The study on electrochemiluminescence of luminol on ITO glass].

The indium tin oxide(ITO) glass was applied as the electrode to study the electrochemilumuinescent behavior of luminol on its surface. The experimental results indicated that it was an excellent electrode material for ECL of luminol with very high sensitivity. The detection limit of the luminol is as low as 10(-15) mol x L(-1) order of magnitude.

[Determination of I- by the ECL of luminol in neutral medium].

Based on the studies of electrochemiluminescence(ECL) of luminol in neutral medium, the iodide would greatly sensitize the ECL of luminol. The ECL luminous intensity responded linearly to the concentration of iodide within the range of 3.8 x 10(-7) mol x L(-1) to 2.

[The determination of glutathione by electrochemiluminescence quenching method].

Glutathione is an important active substance in organisms. Its study and determination are very significant to human being's health and life. In neutral medium, the quenching effect of reduced glutathione on the electrochemiluminescence of luminol-iodide was studied in this paper.

[Determination of total effective value of flavonoids in propolis by quenched electrochemiluminescence analysis].

Propolis is a kind of quality health foodstuff containing flavonoids, which is an important clinical effective component in many Chinese traditional medicines. The flavonoids could be used to reduce or clear free radicals, which manifests its medical functions. But there are no satisfactory methods to analyze the content of flavonoids in propolis.

[UV-vis spectroscopic study of the effect of Cu(II) ions on dopachrome].

The effect of Cu(II) ions on dopachrome has been studied by means of UV-Vis spectra in buffer solution at pH 6.5. Cu(II) ions markedly accelerated the rate of bleaching of dopachrome (DC) at 475 nm and simultaneously exhibited general absorption in the range of 300-800 nm in the system with oxygen removed by bubble nitrogen gas.

[Spectrophotometric determination of vanadium(V) through the formation of a tungstovanadophosphate-TMBPS charge transfer complex].

A new spectrophotometric method was developed for the determination of vanadium(V). The method was based on the formation of tungstovanadophosphate-3,3',5,5'-tetramethylbenzidine-N-propanesulfonic (TMBPS) charge transfer complex. The spectrophotometric measurements were directly carried out at 450 nm and the apparent molar absorptivity was 2.

A nano-molar sensitive disposable biosensor for determination of dopamine.

The studies on a nano-molar sensitive disposable biosensor based on the screen-printing basal electrode and the supramolecular assembly of dual mediators, tetramethylbenzidine and ferrocene, by beta-cyclodextrin inclusion complexation have been reported in this paper. This biosensor shows the high quality of analytical performance because of the complexation and the microenvironment in the supramolecular structure. The extracted protein of mushroom, which contains abundant polyphenol oxidase, was immobilized in the membrane of cross-linked cyclodextrin film.