Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model.

A rapid, sensitive and specific test for blood is reported based on a novel application of recombinase polymerase amplification integrated with CRISPR-Cas and lateral flow assay (LFA). The blood specific marker ALAS2 was used as the target to record the presence of blood. The assay used either RNA extracted from a body fluid as a template, or omitting this extraction step and using a direct approach where the questioned body fluid was added directly to the assay.

Survey on production quality of electrodialysis reversal and reverse osmosis on municipal wastewater desalination.

Water shortage has become an emerging environmental issue. Reclamation of the effluent from municipal wastewater treatment plant (WWTP) is feasible for meeting the growth of water requirement from industries. In this study, the results of a pilot-plant setting in Futian wastewater treatment plant (Taichung, Taiwan) were presented.