Biosensor-based dual-color droplet microfluidic platform for precise high-throughput screening of erythromycin hyperproducers.

Biosensor-based droplet microfluidic high-throughput screening is extensively utilized in engineering microbial cell factories for the efficient production of various natural products. Under ideal conditions, biosensors detect product concentrations in the environment and emit corresponding measurable signals. However, bacteria cell growth rates and gene expression are significantly regulated in response to fluctuating environments, leading to substantial heterogeneity in cell density and gene expression among different subpopulations.

Preparation of Hybrid Molecularly Imprinted Polymers Based on 3-Triethoxysilylpropyl Methacrylic Amide for Solid-Phase Extraction of Gatifloxacin From Lake Water.

A novel molecularly imprinted polymer (MIP) against gatifloxacin based on a hybrid monomer (3-triethoxysilylpropyl methacrylic amide, APTES-MAA) was reported. In this study, the imprinted monolith was synthesized with gatifloxacin as template, ethylene glycol dimethacrylate (EGDMA) as cross-linker, and APTES-MAA as functional monomer in a mixture of acetonitrile and isooctane as porogen via an optimization based on density functional theory (DFT). The polymers were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and mercury porosimetry.

NLRP4 unlocks an NK/macrophages-centered ecosystem to suppress non-small cell lung cancer.

Background: Tumor immune evasion extends beyond T cells, affecting innate immune elements like natural killer cells (NK) and macrophages within the tumor-immune microenvironment (TIME). Nevertheless, translational strategies to trigger collaboration of NK cells and macrophages to initiate sufficient anti-tumor cytoxicity remain scarce and are urgently needed.

Methods: In this study, TCGA datasets was used to confirm the prognosis value of the expression level of NLR family pyrin domain containing 4 (NLRP4) in NSCLC and the tumor tissues microarray was used to further check its clinical-relevance at protein-level.

Comparative proteogenomics reveals ecological and evolutionary insights into the organohalide-respiring strain T.

1,1,1-Trichloroethane (1,1,1-TCA) and chloroform (CF) are persistent groundwater contaminants because of their widespread industrial use as organic solvents and improper disposal in the past. Obligate organohalide-respiring bacteria (OHRB), such as (), play crucial roles in biotransforming and detoxifying natural or anthropogenic halogenated organics including 1,1,1-TCA and CF through reductive dechlorination. Despite their significance, only five strains have been associated with the reductive dechlorination of 1,1,1-TCA or CF.

Multi-omics analyses of the gut microbiota and metabolites in children with metabolic dysfunction-associated steatotic liver disease.

The development and severity of metabolic dysfunction-associated steatotic liver disease (MASLD) in children are closely related to alterations of gut microbiota. This study aims to investigate changes in the gut microbiota signature and microbial metabolites in children with MASLD. We collected fecal samples from children and adolescents aged 6-16 years, and the presence of MASLD was diagnosed by ultrasound.

The impact of physical activity on inhibitory control of adult ADHD: a systematic review and meta-analysis.

Objective: We aimed to Investigate physical activity's effects on inhibitory control in adult attention deficit hyperactivity disorder (ADHD).The benefits of physical activity on the inhibitory control of adult ADHD were explored in the hope of providing some suggestions for approaches to treating adult ADHD.

Methods: We searched the databases PubMed, Web of Science, CNKI and Wanfang for randomised controlled trials (RCTs) of the effect of physical activity on inhibitory control in adults with ADHD, using PRISMA guidelines.

How does internet use affect the sense of gain in older adults? A moderated mediation model.

In the context of the digital era, Internet use is of major significance for enhancing the sense of gain among elderly people and enabling them to share in the development dividend of the digital society. Based on CGSS 2021 ( = 2,929), this study explores the impact of Internet use on the sense of gain in the elderly, and a moderated mediation model was constructed to explore the mediating role of perceived social justice and the potential moderating role of social status. The results showed that (1) Internet use in general significantly enhanced elderly's sense of gain.

Single-cell transcriptome analysis reveals cellular heterogeneity in the aortas of Takayasu arteritis.

Objectives: Takayasu arteritis (TAK) is an inflammatory vasculitis that affects the aorta and its primary branches. The pathogenesis of TAK remains elusive, yet identifying key cell types in the aorta of TAK patients is crucial for uncovering cellular heterogeneity and discovering potential therapeutic targets.

Methods: This study utilized single-cell transcriptome analysis on aortic specimens from three TAK patients, with control data sourced from a publicly available database (GSE155468).

TLR4-mediated endoplasmic reticulum stress regulates pyroptosis in macrophages infected with the Bacillus Calmette-Guérin mycobacterial.

Tuberculosis results from Mycobacterium tuberculosis (Mtb) infection. Immune responses controlled by Toll-like receptor 4 (TLR4) are closely associated with the host response to pathogens, including Mtb. NLRP3 inflammasome-mediated pyroptosis forms a significant part of the inflammatory response during Mtb infection, and endoplasmic reticulum stress (ERS) is implicated in the activation of the NLRP3 inflammasome.

Awake Prone Positioning in Adults With COVID-19: An Individual Participant Data Meta-Analysis.

Importance: The impact of awake prone positioning (APP) on clinical outcomes in patients with COVID-19 and acute hypoxemic respiratory failure (AHRF) remains uncertain.

Objective: To assess the association of APP with improved clinical outcomes among patients with COVID-19 and AHRF, and to identify potential effect modifiers.

Data Sources: PubMed, Embase, the Cochrane Library, and ClinicalTrials.

SE(3)-Equivariant Ternary Complex Prediction Towards Target Protein Degradation.

Targeted protein degradation (TPD) induced by small molecules has emerged as a rapidly evolving modality in drug discovery, targeting proteins traditionally considered "undruggable". Proteolysis-targeting chimeras (PROTACs) and molecular glue degraders (MGDs) are the primary small molecules that induce TPD. Both types of molecules form a ternary complex linking an E3 ligase with a target protein, a crucial step for drug discovery.

Robust Spatial Cell-Type Deconvolution with Qualitative Reference for Spatial Transcriptomics.

Many spatially resolved transcriptomic technologies have been developed to provide gene expression profiles for spots that may contain heterogeneous mixtures of cells. To decompose cellular composition and expression levels, various deconvolution methods have been developed using single-cell RNA sequencing (scRNA-seq) data with known cell-type labels as a reference. However, in the absence of a reliable reference dataset or in the presence of heterogeneous batch effects, these methods may introduce bias.

Association of prenatal exposure to perfluoroalkyl and polyfluoroalkyl substances with fetal growth trajectories.

Background: Maternal perfluoroalkyl and polyfluoroalkyl substances (PFAS) exposure during pregnancy is linked to adverse birth outcomes; however, its impact on fetal growth trajectories remains unclear.

Objective: To examine the relationship between prenatal PFAS exposure and fetal growth trajectories.

Methods: We included 352 mother-infant pairs from a prospective study conducted at Changsha Hospital for Maternal and Child Health Care, Hunan Province, China.

Bioinspired Cu/Zn-ZIF nanozyme with excellent laccase-like activity for selective colorimetric detection of phenolic pollutants.

The ingenious design of active sites in mimetic enzymes is crucial for developing enzyme-like functional materials with high activity and selectivity. Inspired by the N-ligand-rich copper centers of natural laccase, a novel laccase-like nanozyme was developed by loading copper ions into zeolite imidazolate framework-8 (Cu/Zn-ZIF). Benefiting from the precise mimicry of the catalytic center and the high dispersion of catalytic sites which were supported by the MOF backbone, Cu/Zn-ZIF manifested superior laccase-like activity.

Rewilding catalyzes maturation of the humoral immune system.

Inbred mice used for biomedical research display an underdeveloped immune system compared with adult humans, which is attributed in part to the artificial laboratory environment. Despite representing a central component of adaptive immunity, the impact of the laboratory environment on the B cell compartment has not been investigated in detail. Here, we performed an in-depth examination of B cells following rewilding, the controlled release of inbred laboratory mice into an outdoor enclosure.

A local adaptive fuzzy spectral clustering method for robust and practical clustering.

Traditional spectral clustering algorithms are sensitive to the similarity matrix, which impacts their performance. To address this, a local adaptive fuzzy spectral clustering (FSC) method is introduced, incorporating a fuzzy index to reduce this sensitivity. FSC also simplifies the traditional process through a local adaptive framework, optimizing the similarity matrix's use.

Central Thalamic Deep Brain Stimulation Modulates Autonomic Nervous System Responsiveness in Disorders of Consciousness.

Background: The heart rate variability (HRV) of patients with disorders of consciousness (DOC) differs from healthy individuals. However, there is rarely research on HRV among DOC patients following treatment with deep brain stimulation (DBS). This study aims to investigate the modulatory effects of DBS-on the central-autonomic nervous system of DOC based on the study of HRV variations.

Risk factors for sarcopenia in community setting across the life course: A systematic review and a meta-analysis of longitudinal studies.

Background: Sarcopenia is generally an age-related condition in older people that impacts adverse health consequences in terms of quality of life, morbidity and mortality. With the increasing interest of clinical and research interest in sarcopenia internationally, the epidemiological evidence reveals sarcopenia risk begins in early adulthood, influenced by gene, lifestyle and a range of chronic conditions across life course.

Objectives: The purpose of this study was to systematically summarize the risk factors for sarcopenia across the life course, and to identity the high-risk population.

Evaluation of FXR Activity in Pollutants Identified in Sewage Sludge and Subsequent and Characterization of Metabolic Effects of Triphenyl Phosphate.

Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common liver disease worldwide, and increasing evidence suggests that exposure to environmental pollutants is associated with the increased incidence of MASLD. The farnesoid X receptor (FXR) plays an important role in the development of MASLD by regulating bile acids (BAs) and lipid metabolism. However, whether FXR-active pollutants are the environmental drivers of MASLD remains unclear.

[Research progress of ICI and CAR-T in tumor immunotherapy].

Tumor immunotherapy has revolutionized the treatment prospects for various malignant tumors. Immune checkpoint inhibitors (ICIs) and chimeric antigen receptor T-cell therapy (CAR-T) , as representative of tumor immunotherapy, have achieved tremendous success in clinical practice and have become the first-line clinical treatment options for certain tumors. This article summarizes the progress and challenges of immune checkpoint inhibitors and CAR-T therapy in tumor treatment, and discusses the future direction of tumor therapeutic vaccines development.

Clinical outcomes and pharmacokinetics/pharmacodynamics of intravenous polymyxin B treatment for various site carbapenem-resistant gram-negative bacterial infections: a prospective observational multicenter study.

Polymyxin B, a last resort for carbapenem-resistant gram-negative bacteria (CRGNB) infections, has infection site-specific pharmacokinetic/pharmacodynamic (PK/PD) properties. However, there is little clinical evidence to support optimal exposures of polymyxin B for different site infections. We performed a prospective, observational, multicenter study to evaluate the clinical outcomes and PK/PD of intravenous polymyxin B treatment for various site CRGNB infections.

A case of aortic root replacement for acute type A dissection in Marfan syndrome with twin pregnancy.

Type A aortic dissection is a life-threatening condition requiring urgent surgery. However, clinical experience with type A aortic dissection during twin pregnancy is extremely limited. We report a case of a 32-year-old woman with Marfan syndrome who was diagnosed with type A aortic dissection at 37 weeks of gestation with boy-girl twins.

Qingre Huayu Jianpi prescription alleviates the inflammatory transformation of colitis-associated colorectal cancer by inhibiting the IL-17RA/ACT1/NF-κB axis.

Ethnopharmacological Relevance: Inflammation-to-cancer transformation is critical for the progression of ulcerative colitis to colitis-associated colorectal cancer (CAC).

Aim Of The Study: To explore the role and potential mechanisms of Qingre Huayu Jianpi prescription (QHJ) treatment in the development of CAC.

Materials And Methods: Combined network pharmacology and transcriptome analyses were used to investigate QHJ-associated targets and pathways in the context of CAC.

ABVS breast tumour segmentation via integrating CNN with dilated sampling self-attention and feature interaction Transformer.

Given the rapid increase in breast cancer incidence, the Automated Breast Volume Scanner (ABVS) is developed to screen breast tumours efficiently and accurately. However, reviewing ABVS images is a challenging task owing to the significant variations in sizes and shapes of breast tumours. We propose a novel 3D segmentation network (i.