Analysis of genotypic distribution and rare variants of patients with α/β-thalassemia screened in one hospital in Beijing, China.

Objective: Thalassemia is among the most common inherited diseases worldwide. We aimed to analyze the genotype and frequency distribution of thalassemia in a general hospital in Beijing and provide a reference for genetic counseling and prenatal diagnosis.

Methods: A total of 3196 cases of thalassemia screened at Peking Union Medical College Hospital (PUMCH) between January 2018 and January 2022 were collected.

A New target of ischemic ventricular arrhythmias-ITFG2.

ITFG2 is an intracellular protein known to modulate the immune response of T-cells. Our previous investigation revealed that ITFG2 specifically targets ATP5b to regulate ATP energy metabolism and maintain mitochondrial function, thereby protecting the heart from ischemic injury. However, the role of ITFG2 in ischemic ventricular arrhythmias and its underlying mechanisms have not been previously reported.

Duration of Hypothermia is Associated with Postoperative Complications in Patients Undergoing Gynecological Surgery: A Prospective Cohort Study.

Objectives: To investigate the relationship between hypothermia duration and postoperative complications in patients undergoing gynecological surgery.

Methods: Patients who underwent elective gynecological surgery at our hospital were consecutively enrolled between October 2020 and January 2022. Core temperature was continuously monitored intraoperatively, and early postoperative complications were collected.

Digitalized 3D Spinal Decompression and Correction Device Improved Initial Brace Corrections and Patients' Comfort Among Adolescents with Idiopathic Scoliosis: A Single-Centre, Single-Blinded Randomized Controlled Trial.

This study aimed to evaluate the efficacy of a novel three-dimensional (3D) spinal decompression and correction device in improving the in-brace correction and patient comfort level for adolescents with idiopathic scoliosis (AIS), and to assess the impact of the number of vertebrae involved in the scoliotic curve on the correction's effectiveness. A single-centre, single-blinded randomized controlled trial (RCT) was conducted in 110 AIS patients aged 10-18 years who were randomly allocated into four groups receiving 0-3 days of device intervention. Each session lasted for 30 min and was conducted twice daily.

The Biomechanics of Spinal Orthoses for Adolescent Idiopathic Scoliosis: A Systematic Review of the Controlling Forces.

Background: Orthotic treatment is a well-acknowledged conservative treatment for moderate adolescent idiopathic scoliosis (AIS). The efficacy of this treatment is significantly determined by the forces applied to the bodies of patients. However, there is uncertainty regarding the optimal force levels that should be applied to the patient's torso by spinal orthosis.

A novel efficient liquefaction process for corn starch through ternary deep eutectic solvent: Products characterization and liquefaction mechanism.

A novel approach for the solvothermal liquefaction of corn starch (CS) was investigated, using ternary deep eutectic solvent (TDES) as both an acidic catalyst and a source of liquefaction reagent. Synergistic effects from multi-component TDES were observed, leading to milder reaction conditions (110 °C, 35 min) and improved product selectivity (relative content of polyhydroxy compounds up to 97.83 %).

Evaluation of three rapid assays for detecting Mycoplasma pneumoniae in nasopharyngeal specimens.

During the 2023 autumn-winter period in China, Mycoplasma pneumoniae (MP) infections have increased. To address this, rapid and accurate MP DNA detection methods are crucial. Three nucleic acid detection assays (Ustar, Coyote Flash10, Coyote Flash 20) that are widely used in China are currently being evaluated for their effectiveness in detecting MP DNA in nasopharyngeal specimens.

Highly efficient esterification of waxy maize starch in choline chloride/acetic acid acidic deep eutectic solvent system.

In this study, to address the issue of solvent selection in the chemical modification of starch, a method was developed for the efficient esterification of waxy maize starch (WMS) using an acidic deep eutectic solvent composed of choline chloride and acetic acid (CCHAc-ADES). The impact of different mass fractions of CCHAc-ADES on the degree of substitution and reaction efficiency of lauric acid starch esters was explored. It was found that under the conditions of 70 wt% CCHAc-ADES, starch esters with the highest degree of substitution of 0.

Analytical Performance of a Novel Nanopore Sequencing for SARS-CoV-2 Genomic Surveillance.

The genomic analysis of SARS-CoV-2 has served as a crucial tool for generating invaluable data that fulfils both epidemiological and clinical necessities. Long-read sequencing technology (e.g.

Cardiopulmonary bypass of acute type A aortic dissection during pregnancy at the 20th gestational week with maternal and foetal survival: A case report.

Background: Type A acute aortic dissection, an aggressive and highly fatal disease, is particularly common among pregnant women and foetuses. This disease often occurs during the third trimester of pregnancy and the postpartum period.

Case Report: This case involves a female who was 20 weeks pregnant with type A acute aortic dissection.

Deformation Control in Mesoscale Micro-Milling of Curved Thin-Walled Structures.

The micro-machining scale effect makes it challenging to forecast and control the process parameters of the micro-milling process, which makes the micro-flanking-milling of weak-rigidity micro-thin-walled parts prone to deformation. To determine the critical cutting parameters for chip formation in the micro-milling of curved thin-walled parts at the mesoscale, the strain-softening effect of titanium alloy during high-speed milling and the scale effect of mesoscale cutting were comprehensively considered and a finite element prediction model for curved micro-thin-wall micro-milling was established to determine the critical milling parameters for effective material removal. Based on the determined milling parameters, an experimental design of response surface optimization was carried out.

Influence of COVID-19 pandemic on prevalence and genotype distribution of HPV in cervical cancer screening population.

Background: Human Papillomavirus (HPV) DNA screening was a crucial element in the fight against cervical cancer and had been adopted in many countries, including China. However, the onset of the COVID-19 pandemic in March 2020 disrupted this program significantly.

Methods: The aim of this study is to investigate the prevalence and distribution of HPV genotypes among the population undergoing cervical cancer screening during the pandemic period.

Chinese Anesthesiology Milestones in Resident Evaluation: Reliability, Validity, and Correlation with Objective Examination Scores: a Cross-sectional Study.

Background: Evaluating competency acquisition during residency training is crucial. The Anesthesiology Milestones have been implemented in the United States. The China Consortium of Elite Teaching Hospitals for Residency Education has also developed the Chinese Resident Core Competency Milestone Evaluation System.

Photothermal driven BMSCs osteogenesis and M2 macrophage polarization on polydopamine-coated TiC nanosheets/poly(vinylidene fluoride trifluoroethylene) nanocomposite coatings.

Mild thermal stimulation plays an active role in bone tissue repair and regeneration. In this work, a bioactive polydopamine/TiC/poly(vinylidene fluoride trifluoroethylene) (PDA/TiC/P(VDF-TrFE)) nanocomposite coating with excellent near-infrared light (NIR)-triggered photothermal effect was designed to improve the osteogenic ability of implants. By incorporating dopamine (DA)-modified TiC nanosheets into the P(VDF-TrFE) matrix and combining them with alkali initiated in situ polymerization, the resulting PDA/TiC/P(VDF-TrFE) nanocomposite coating gained high adhesion strength on Ti substrate, excellent tribological and corrosion resistance properties, which was quite important for clinical application of implant coatings.

Clinical diagnostic value of targeted next‑generation sequencing for infectious diseases (Review).

As sequencing technology transitions from research to clinical settings, due to technological maturity and cost reductions, metagenomic next‑generation sequencing (mNGS) is increasingly used. This shift underscores the growing need for more cost‑effective and universally accessible sequencing assays to improve patient care and public health. Therefore, targeted NGS (tNGS) is gaining prominence.

Temperature effect on coagulation function in mild hypothermic patients undergoing thoracic surgeries: thromboelastography (TEG) versus standard tests.

Purpose: Our previous research has revealed that mild hypothermia leads to excessive bleeding in thoracic surgeries, while the underlying mechanism stayed unrevealed by the standard coagulation tests. The research question in this study was as follows: "How does mild hypothermia impair the hemostatic function in patients receiving thoracic surgeries?". The purpose was to detect the disturbed coagulation processes by comparing the TEG parameters in patients receiving active vs.

Emerging role of mesenchymal stem cell-derived exosome microRNA in radiation injury.

Purpose: Radiation injury (RI) is a common occurrence in malignant tumors patients receiving radiation therapy. While killing tumor cells, normal tissue surrounding the target area is inevitably irradiated at a certain dose, which can cause varying results of radiation injury. Currently, there are limited clinical treatments available for radiation injuries.

Individualized rotation of left double lumen endobronchial tube to improve placement success rate: a randomized controlled trial.

Background: In conventional practice, the left double lumen tube (DLT) is rotated 90° counterclockwise when the endobronchial cuff passes glottis. Success rate upon the first attempt is < 80%, likely owing to varying morphology of the bronchial bifurcation.

Methods: We conducted a randomized controlled trial to compare 90° counterclockwise rotation versus individualized degree of rotation in adult patients undergoing elective thoracic surgery using left DLT.

Cocirculation and coinfection of multiple respiratory viruses during autumn and winter seasons of 2023 in Beijing, China: A retrospective study.

China experienced severe epidemics of multiple respiratory pathogens in 2023 after lifting "Zero-COVID" policy. The present study aims to investigate the changing circulation and infection patterns of respiratory pathogens in 2023. The 160 436 laboratory results of influenza virus and respiratory syncytial virus (RSV) from February 2020 to December 2023, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from June 2020 to December 2023, Mycoplasma pneumoniae, adenovirus, and human rhinovirus from January 2023 to December 2023 were analyzed.

Low expression of interleukin-1 receptor antagonist correlates with poor prognosis via promoting proliferation and migration and inhibiting apoptosis in oral squamous cell carcinoma.

Background: Biomarkers are biochemical indicators that can identify changes in the structure or function of systems, organs, or cells and can be used to monitor a wide range of biological processes, including cancer. Interleukin-1 receptor antagonist (IL1RA) is an important inflammatory suppressor gene and tumor biomarker. The goal of this study was to investigate the expression of IL1RA, its probable carcinogenic activity, and its diagnostic targets in oral squamous cell carcinoma (OSCC).