CRISPR/Cas-mediated genome editing in mice for the development of drug delivery mechanism.

Background: To manipulate particular locations in the bacterial genome, researchers have recently resorted to a group of unique sequences in bacterial genomes that are responsible for safeguarding bacteria against bacteriophages. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) are two such systems, each of which consists of an RNA component and an enzyme component.

Methods And Results: This review focuses primarily on how CRISPR/Cas9 technology can be used to make models to study human diseases in mice.