Optimization of FRET imaging in Arabidopsis Protoplasts.

Recent advancements in fluorescence-based biosensor technologies have enabled more precise and accurate Förster Resonance Energy Transfer (FRET) imaging within Agrobacterium-mediated plant transformation systems. However, the application of FRET imaging in plant tissues remains hindered by significant challenges, particularly the time-intensive process of generating transgenic lines and the complications arising from tissue autofluorescence. In contrast, protoplast-based FRET imaging offers a rapid and efficient platform for functional screening and analysis, making it an essential tool for plant research.