RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer Cells.

Background: Receptor Expressed in Lymphoid Tissues (RELT) is a TNFRSF member that has two paralogs, RELL1 and RELL2; the three proteins are collectively referred to as RELT family members (RELTfms).

Methods: We sought to evaluate RELT expression in cancerous cells by using real-time PCR, western blotting, flow cytometry, and immunohistochemistry (IHC). The mechanism of RELT-induced cell death was assessed by western blotting, flow cytometry, luciferase assays, and morphology staining.

Repeatability of Corneal Hysteresis Measurements in Glaucoma Patients During Routine Follow Up and After Cataract Surgery.

Prcis: Corneal hysteresis (CH) measurements are relatively stable during routine follow up of glaucoma patients over an intermediate time frame. Cataract surgery does not change the CH significantly after an average follow up of 6 months.

Purpose: The aim was to assess the repeatability of CH measurements in glaucoma patients over time, during routine follow up and after cataract surgery.

Spectrally Resolved, Functional Super-Resolution Microscopy Reveals Nanoscale Compositional Heterogeneity in Live-Cell Membranes.

By recording the full fluorescence spectra and super-resolved positions of ∼10 individual polarity-sensing solvatochromic molecules, we reveal compositional heterogeneity in the membranes of live mammalian cells with single-molecule sensitivity and ∼30 nm spatial resolution. This allowed us to unveil distinct polarity characteristics of the plasma membrane and the membranes of nanoscale intracellular organelles, a result we found to be due to differences in cholesterol levels. Within the plasma membrane, we observed the formation of low-polarity, raft-like nanodomains upon cholesterol addition or cholera-toxin treatment, but found this nanoscale phase separation absent in native cells.