ADA2 isoform of adenosine deaminase from pleural fluid.

Adenosine deaminase isoenzyme 2 (ADA2) was isolated from human pleural fluid for the first time. Molecular and kinetic properties were characterized. It was shown that the inhibitors of adenosine deaminase isoenzyme 1 (ADA1), adenosine, and erithro-9-(2-hydroxy-3-nonyl)adenine (EHNA) derivatives are poor inhibitors of ADA2.

Activity of adenosine deaminase and its isoforms in pleural fluid in tuberculous pleuritis.

Background: The problem of tuberculosis is increasing in a number of countries. Adenosine deaminase activity is considered in many clinics to be a valuable biochemical test of this pathology. Considerable research has been devoted to the activity of enzyme isoforms as significant tests for diagnosing tuberculosis.