Quantum Dot Reporters Designed for CRISPR-Based Detection of Viral Nucleic Acids.

Diagnostic methods based on CRISPR technology have shown great potential due to their highly specific, efficient, and sensitive detection capabilities. Although the majority of the current studies rely on fluorescent dye-quencher reporters, the limitations of fluorescent dyes, such as poor photostability and small Stokes shifts, urgently necessitate the optimization of reporters. In this study, we developed innovative quantum dot (QD) reporters for the CRISPR/Cas systems, which not only leveraged the advantages of high photoluminescence quantum yield and large Stokes shifts of QDs but were also easily synthesized through a simple one-step hydrothermal method.

CRISPR/Cas13a Trans-Cleavage-Triggered Catalytic Hairpin Assembly Assay for Specific and Ultrasensitive SARS-CoV-2 RNA Detection.

New coronavirus (SARS-CoV-2), which has caused the coronavirus disease 2019 (COVID-19) pandemic, has brought about a huge burden on global healthcare systems. Rapid and early detection is important to prevent the spread of the pandemic. Here, an assay based on CRISPR/Cas13a and catalytic hairpin assembly (CHA), termed as Cas-CHA, was developed for ultrasensitive and specific detection of SARS-CoV-2 RNA.

Synthesis of bio-templated clickable quantum dots and a dual-emitting organic/inorganic complex for ratiometric fluorescence visual assay of blood glucose.

With the prevalence of diabetes, rapid and simple blood glucose monitoring has become more and more important. Here, we report the synthesis of bio-templated N-CdZnTeS quantum dots (QDs), which are great fluorescent biological labels and were used for the fabrication of dual-emissive dye@protein-QD conjugates copper-free click chemistry, such as the 5(6)-carboxyfluorescein@glucose oxidase-quantum dot (FAM@GOx-QDs) complex. When adding glucose, the red fluorescence of the CdZnTeS QDs sharply decreased, while the green fluorescence of FAM was invariable.

Investigating the effect of 6-mercaptohexanol on the performance of a biosensor based on nanosurface energy transfer between gold nanoparticles and quantum dots.

Nanosurface energy transfer (NSET)-based sensors have been widely developed using various pairs of nanomaterials including gold nanoparticles (AuNPs) and quantum dots (QDs). However, a low signal to background ratio is one of the most important problems that researchers are continually trying to solve. Herein, we present a 6-mercaptohexanol (MCH) modified MCH/DNA-Au-QD sensor for the detection of nucleic acids and MUC1.

Point-of-care testing (POCT) of patients with a high concentration of uric acid by using alginate hydrogel microspheres embedded with CdZnTeS QDs and urate oxidase (Alg@QDs-UOx MSs).

High concentration of uric acid is usually related to cardiovascular and cerebrovascular diseases. Developing a simple method for the rapid and efficient detection of uric acid has a great significance in clinical diagnosis. In this work, alginate hydrogel microspheres embedded with CdZnTeS QDs and urate oxidase (Alg@QDs-UOx MSs) were prepared for the first time, and further used for point-of-care testing (POCT) of patients with a high concentration of uric acid.

Homogeneous immunoassay for alpha-fetoprotein based on the quenching of the fluorescence of quantum dots by antibody labelled with complexed copper ion tags.

A homogeneous fluorescent immunoassay is described for the determination of alpha fetoprotein (AFP) relying on the interaction between copper ion complex and quantum dots (QDs). The copper ion complex-labelled antibody can be employed as a quencher of fluorescence of QDs and capture probe of AFP in homogeneous solution. The labelled antibody is mixed with QDs to form the immune ensemble probe.

DNA-templated quantum dots and their applications in biosensors, bioimaging, and therapy.

Over the past 10 years, DNA functionalized quantum dots (QDs) have attracted considerable attention in sensing and imaging of disease-relevant biological targets, as well as cancer therapy. Considerable efforts have been devoted to obtaining DNA functionalized QDs with enhanced stability and quantum yield. Here, we focus on a one-pot method, in which phosphorothioate-modified DNA is used as the co-ligand on the basis of the strong binding of sulfur and Cd.

Facile synthesis of stable CdTe/CdS QDs using dithiol as surface ligand for alkaline phosphatase detection based on inner filter effect.

Alkaline phosphatase (ALP) is a universal and important hydrolase that has been proved to be associated with several diseases. Herein, a simple and effective method was proposed for ALP detection based on the inner filter effect of p-nitrophenol (pNP) on the fluorescence of CdTe/CdS quantum dots (QDs). For the preparation of CdTe/CdS QDs, NaTeO was used as the Te source, and dithiol as the S source and surface ligand.

7-Meth-oxy-3-(4-meth-oxy-phen-yl)chroman-4-one.

The asymmetric unit of the title compound, C(17)H(16)O(4), contains two crystallographically independent mol-ecules with different absolute configurations.

3-(4-Hy-droxy-phen-yl)-7-meth-oxy-chroman-4-one monohydrate.

In the title compound, C(16)H(14)O(4)·H(2)O, the dihedral angle betwen the benzene rings is 71.4 (6)°. The pyran ring is in a sofa conformation.

4-alkoxy-3-arylfuran-2(5H)-ones as inhibitors of tyrosyl-tRNA synthetase: synthesis, molecular docking and antibacterial evaluation.

A series of novel 4-alkoxy-3-arylfuran-2(5H)-ones as tyrosyl-tRNA synthetase inhibitors were synthesized. Of these compounds, 3-(4-hydroxyphenyl)-4-(2-morpholinoethoxy)furan-2(5H)-one (27) was the most potent. The binding model and structure-activity relationship indicate that replacement of morpholine-ring in the side chain of 27 with a substituent containing more hydrophilic groups would be more suitable for further modification.