Identification of sucrose synthase from to favor biocatalytic glycosylation.

Sucrose synthase (SuSy, EC 2.4.1.

Three Glycosyltransferase Mutants in a One-Pot Multi-enzyme System with Enhanced Efficiency for Biosynthesis of Quercetin-3,4'--diglucoside.

Quercetin-3,4'--diglucoside (Q3,4'G), among the major dietary flavonoids, is superior to quercetin aglycone or quercetin monoglucoside in solubility. However, its low content in nature makes it hard to be prepared in large quantities by traditional extraction methods. In the present study, the F378S mutant of UGT78D2 (78D2_F378S) derived from with improved regioselectivity and the V371A mutant of UGT73G1 (73G1_V371A) derived from were adopted to realize a two-step continuous glycosylation of quercetin to produce Q3,4'G.

Discovering and efficiently promoting the extracellular secretory expression of Thermobacillus sp. ZCTH02-B1 sucrose phosphorylase in Escherichia coli.

Sucrose phosphorylase (SPase, EC2.4.1.