EgSeverin and Eg14-3-3zeta from Echinococcus granulosus are potential antigens for serological diagnosis of echinococcosis in dogs and sheep.

Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by the metacestode larva of Echinococcus granulosus. In this study, two-dimensional gel electrophoresis (2-DE) coupled with immunoblot analysis revealed that E. granulosus severin and 14-3-3zeta proteins (named EgSeverin and Eg14-3-3zeta, respectively) might be two potential biomarkers for serological diagnosis of echinococcosis.

Effect of Daratumumab combined with Chemotherapy on Immune Function in Patients with Relapsed/Refractory Multiple Myeloma and Observation of its Clinical Efficacy.

Objective: To evaluate the clinical efficacy and immune function of Daratumumab combined with chemotherapy in patients with relapsed/refractory multiple myeloma (RRMM).

Methods: Eighty patients with RRMM treated in Xingtai People's Hospital from January, 2020 to December, 2021 were randomly divided into two groups. Patients in the study group were treated with Daratumumab combined with PAD regimen, while patients in the control group were provided with PAD regimen alone.

Temporal metabolic profiling of erythrocytes in mice infected with Babesia microti.

Background: Babesiosis is an emerging zoonosis worldwide that is caused by tick-borne apicomplexans, Babesia spp., which threatens the health of domesticated and wild mammals and even humans. Although it has done serious harm to animal husbandry and public health, the study of Babesia is still progressing slowly.

Seroprevalence and the Risk Factor of Infection to Slaughter Pigs in Chongqing, China.

can infect all mammals, including humans, and can cause serious public health problems and economic losses. Pork is considered an important source of infection for humans, and seroepidemiological surveys are used to assess the level of infection in pig herds. To understand the current seroprevalence and potential risk factors of in pigs in Chongqing, a total of 1221 serum samples collected from seven slaughterhouses in five districts from 2015 to 2019 were analyzed for antibodies against the protozoan by enzyme-linked immunosorbent assay.

miR-506 in patients with chronic myeloid leukemia and its effect on apoptosis of K562 cells.

Objective: To explore the expression of miR-506 in chronic myeloid leukemia (CML) and its influence on the biological function of CML cells.

Methods: Altogether 84 CML patients from February 2012 to September 2014 were obtained as the observation group (OG), and 71 healthy people were taken as the control group (CG). miR-506 was tested using RT-qPCR, and the 5-year survival of patients with high and low expression of miR-506 was compared with the median value of miR-506 as the limit.

miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1.

Objective: To validate the role of miR-497-5p in apoptosis in K562 cells by targeting Rho-associated kinase isoform 1 (ROCK1).

Methods: From January, 2017 to February, 2019, 57 patients with chronic myeloid leukemia (CML) treated in our hospital were included in patient group, and 50 healthy individuals were recruited as control group. miR-497-5p level in peripheral blood was quantitated using qRT-PCR.

The divergent response of vegetation phenology to urbanization: A case study of Beijing city, China.

Characterizing the relationship between vegetation phenology and urbanization indicators is essential to understand the impacts of human activities on urban ecosystems. In this study, we explored the response of vegetation phenology to urbanization in Beijing (China) during 2001-2018, using impervious surface area (ISA) and the information of urban-rural gradients (i.e.

Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China.

Raw or undercooked meat is an important source of Toxoplasma gondii infection in China, but there is little research data on these infections in Chongqing. This study determined the prevalence of T. gondii, and its genotypes, in pork.

Seroprevalence and associated risk factors of Toxoplasma gondii infection in yaks (Bos grunniens) on the Qinghai-Tibetan Plateau of China.

Toxoplasma gondii is an intracellular parasite that is extensively prevalent globally. Studies have indicated the presence of T. gondii infection in animals in some provinces of China, but little is known about T.

Expression and functionality of allergenic genes regulated by simulated gastric juice in Anisakis pegreffii.

To better understand the molecular mechanisms underlying allergens and parasite immunity and discover the stage-enriched gene expression of fish-borne zoonotic nematodes in the stomach, we used RNA-seq to study the transcriptome profiles of Anisakis pegreffii (Nematoda: Anisakidae, AP) in simulated gastric juice. Mobile L3 larvae were incubated in simulated medium at 37 °C in 5% CO (AP-GJ) and the control group larvae were collected in PBS under the same conditions (AP-PBS). We found that the sequences of A.

Low prevalence of spargana infection in farmed frogs in the Yangtze River Delta of China.

Frogs are the main source of infection for human sparganosis. In this study, the prevalence and pathogenicity of plerocercoid larvae (sparganum) in frogs collected from the Yangtze River Delta in East China were investigated. A total of 386 frogs belonging to five species were purchased from farmers' markets across all three provincial level areas in the Yangtze River Delta region.

G allele of rs7853346 polymorphism in PTENP1 enhances the proliferation of multiple myeloma cancer stem cells by promoting the expression of PTENP1 and its downstream signaling molecules.

Noncoding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), are shown to be associated with the pathogenesis of various diseases, such as multiple myeloma (MM). Therefore, the aim of this study was to explore the role of rs7853346 polymorphism in PTENP1, and its downstream signaling molecules, in the pathology of MM. Forty-three multiple myeloma patients and 35 healthy subjects were recruited and divided into CC, CG, and GG groups according to their genotypes of rs7853346 polymorphism in PTENP1.

Prevalence and genetic diversity of Enterocytozoon bieneusi in sheep in China.

Background: Enterocytozoon bieneusi is a common species of microsporidia that not only influences human health but also threatens animal productive performance and value. However, there have been no systematic studies of the prevalence of E. bieneusi in sheep in China.

Sheep as a Potential Source of Zoonotic Cryptosporidiosis in China.

In this study, we assessed the prevalence and genetic characteristics of in sheep from 10 provinces in China. Fecal samples from 1,035 sheep originating from 16 farms were collected, and 295 (28.5%) were found to be positive by nested PCR.

Synthesis of a Poly-l-Lysine/Black Phosphorus Hybrid for Biosensors.

A simple, noncovalent modification strategy was proposed to synthesize poly-l-lysine-black phosphorus (pLL-BP) hybrid. BP nanoflakes were prepared with a water-phase exfoliation method. pLL can adhere to the surface of BP via hydrophobic interaction between butyl chains of pLL and the BP surface as well as the electrostatic interaction between the protonated amino groups on pLL and the negative charge on deprotonated PO groups remaining on BP.

[Study on the Analysis of the Proportion of Mixed Samples with Near Infrared Spectroscopy and Non-Negative Coefficients Regression].

In the process of practical production, it is important to accurately analyze the proportion of mixed samples with high speed, which plays a great role for quality control and formulation design in food and agricultural processing. Traditional solution is to build statistical model with a large number of representative samples, which is both labor-intensive and time-consuming. In this paper, the proportion of alcohol and acids mixed samples, and their dilute solution mixed samples(used carbon tetrachloride (CCl4) which has no near-infrared absorption characteristics as the solvent medium)，as well as sheet tobacco leaf mixed samples are respectively analyzed by using near infrared spectroscopy, SG smooth and non-negative coefficients regression, which verifies the feasibility of analyzing the proportion of the mixed samples.