Publications by authors named "Yehong Gui"
Article Synopsis
- The integrated portable and automatic digital detection system (IPADS) is a new tool designed for rapid and accurate diagnosis of infectious diseases through nucleic acid detection.
- It uses a hybrid magnetic system (HMS) to efficiently extract genetic material, simplifying traditional methods and allowing for quick sample processing with over 80% extraction efficiency.
- In tests for hepatitis B virus (HBV) DNA, IPADS showed strong alignment with standard PCR methods, proving its potential as a reliable option for point-of-care testing.
O157:H7 ( O157:H7) is a prominent pathogenic bacterium that poses serious risks to food safety and public health. Rapid and accurate detection of live O157:H7 is of great importance in food quality monitoring and clinical diagnosis. Here, we report a propidium monoazide-assisted nonamplification digital CRISPR/Cas12a assay for sensitive and rapid detection of live O157:H7.
View Article and Find Full Text PDFO157:H7 is a major foodborne pathogen that poses a significant threat to food safety and human health. Rapid and sensitive detection of viable O157:H7 can effectively prevent food poisoning. Here, we developed a microwell-confined and propidium monoazide-assisted digital CRISPR microfluidic platform for rapid and sensitive detection of viable O157:H7 in food samples.
View Article and Find Full Text PDFMultiplex, sensitive, and rapid detection of pathogens is crucial for ensuring food safety and safeguarding human health, however, it remains a significant challenge. This study proposes a concanavalin A-assisted multiplex digital amplification (CAMDA) assay for simultaneous quantitative detection of multiple foodborne bacteria. The CAMDA assay enables the simultaneous detection of six foodborne pathogens within 1.
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