TRANSCRIPTIONAL ANALYSIS OF ZINC-DEPENDENT HISTONE DEACETYLASES IN SEVERAL HUMAN CANCER CELLS.

Unlabelled: Histone deacetylases, especially zinc-dependent deacetylases HDACs, are among attractive drug targets for treating cancer in recent years.

Aim: To explore the expression level of HDACs in several human cancer cell lines and examine the possible association between their expression and the sensitivity/resistance to the selective- or pan-HDAC inhibitors.

Materials And Methods: The RNA expression of 11 HDACs isoforms was assayed in HeLa, HepG2, AV3, HEK293, A549, and K562 cells by semiquantitative reverse transcription-polymerase chain reaction.

The effect of training orthopedic nurse specialists in Jiangsu Province of China.

Background: The training of orthopedic nurse specialists is gradually increasing in China, but the effect of the training, namely the specialized nursing work carried out by them upon returning to work after training, is unknown. This study aimed to further our understanding of the effect of training orthopedic nurse specialists and provide a basis for improving the training program.

Methods: A total of 201 clinical nurse specialists who graduated from the training base of orthopedic nurse specialists in the Jiangsu Province were interviewed via a self-made questionnaire.

A Series of Novel HDAC Inhibitors with Anthraquinone as a Cap Group.

Although anthraquinone derivatives possess significant antitumor activity, most of them also displayed those side effects like cardiotoxicity, mainly owing to their inhibition of topoisomerase II of DNA repair mechanisms. Our raised design strategy by switching therapeutic target from topoisomerase II to histone deacetylase (HDAC) has been applied to the design of anthraquinone derivatives in current study. Consequently, a series of novel HDAC inhibitors with a tricylic diketone of anthraquinone as a cap group have been synthesized.

N -acetyl lysine derivatives with zinc binding groups as novel HDAC inhibitors.

HDAC inhibitors have been developed very rapidly in clinical trials and even in approvals for treating several cancers. However, there are few reported HDAC inhibitors designed from N -acetyl lysine. In the current study, we raised a novel design, which concerns N -acetyl lysine derivatives containing amide acetyl groups with the hybridization of ZBG groups as novel HDAC inhibitors.

The mimics of N-acyl-lysine derived from cysteine as sirtuin inhibitors.

Sirtuin inhibitors as physiological research tools and therapeutic potentials have caught many attentions in last decades. The mimics of acyl lysine have been approved to be a very efficient strategy for development of mechanism-based sirtuin inhibitors. In current study, a novel scaffold of l-S-(3-carboxamidopropyl) cysteine (l-CAPC) has been exploited for design and synthesis of sirtuin inhibitors.