Endophytic Herbaspirillum seropedicae expresses nif genes in gramineous plants.

Abstract The interactions between maize, sorghum, wheat and rice plants and Herbaspirillum seropedicae were examined microscopically following inoculation with the H. seropedicae LR15 strain, a Nif(+) (Pnif::gusA) mutant obtained by the insertion of a gusA-kanamycin cassette into the nifH gene of the H. seropedicae wild-type strain.

Microscopic and NMR analysis of the external coat from seeds of Magonia pubescens.

•  The chemical composition and structure of the layers of the external coat and the three-dimensional organization of the gel during the swelling process are reported here for seeds of Magonia pubescens. •  Histochemical-light and scanning electron microscopy (SEM) were used in conjunction with CP-MAS (cross polarization - magic angle spinning: solid state) C NMR spectroscopy to analyse the seed coat layers. •  M.