Inhibition of the STAT3 Signaling Pathway Contributes to the Anti-Melanoma Activities of Shikonin.

Background: Malignant melanoma is an extremely aggressive and metastatic cancer, and highly resistant to conventional therapies. Signal transducer and activator of transcription 3 (STAT3) signaling promotes melanoma development and progression, which has been validated as an effective target in melanoma treatment. Natural naphthoquinone shikonin is reported to exert anti-melanoma effects.

Fast enantioselective determination of triadimefon in different matrices by supercritical fluid chromatography.

A fast analytical method through supercritical fluid chromatography was developed for enantioselective determination of triadimefon in soil and apple samples. Effects of chiral stationary phases, co-solvents, column temperature, and back pressure on chiral separation of triadimefon were discussed in detail. Chiral stationary phases containing electron-donating units located at phenylcarbamate moieties showed better enanitonrecognition abilities in triadimefon than those with electron-withdrawing units.

Lenalidomide, a blockbuster drug for the treatment of multiple myeloma: Semipreparative separation through supercritical fluid chromatography and vibrational circular dichroism spectroscopy.

Semipreparative separation of lenalidomide has been performed through supercritical fluid chromatography. In regard to retention and resolution of lenalidomide, effects of chromatographic conditions, such as chiral stationary phases, organic co-solvents, mobile phases, and column temperature, have been studied in detail. Amylose tris(3, 5-dimethylphenylcarbamate)-coated and the single-urea-bound β-cyclodextrin chiral stationary phases exhibited good separation performances for lenalidomide in the CO /methanol mixture.

Efficient preparative separation of β-cypermethrin stereoisomers by supercritical fluid chromatography with a two-step combined strategy.

An efficient two-step method has been developed for the separation of β-cypermethrin stereoisomers by supercritical fluid chromatography with polysaccharide chiral stationary phases. With respect to retention, selectivity, and resolution of β-cypermethrin, the effects of chiral stationary phases, cosolvents, mobile phases, and column temperature have been studied in detail. Through a two-step separation, β-cypermethrin was firstly separated by using a cellulose-derived chiral stationary phase to obtain two stereoisomeric pairs, and further resolved on an amylose-based chiral stationary phase to produce four enantiopure stereoisomers.

Enantioselective determination of metconazole in multi matrices by high-performance liquid chromatography.

A reliable and effective HPLC analytical method has been developed to stereoselectively quantify metconazole in soil and flour matrices. Effects of polysaccharide chiral stationary phase, type and content of alcoholic modifier on separation of racemic metconazole have been discussed in detail. Resolution and quantitative determination of metconazole stereoisomers were performed by using an Enantiopak OD column, with the n-hexane-ethanol mixture (97:3, v/v) at the flow rate of 1.