Publications by authors named "Ye-Hui Lu"
Article Synopsis
- Forensic genetics labs are increasingly using massively parallel sequencing (MPS) technology, including next-generation sequencing (NGS), to analyze various genetic markers for applications like individual identification and ancestry analysis.
- MPS offers significant advantages over traditional capillary electrophoresis (CE), such as the ability to detect many genetic markers simultaneously and better resolution, but it is also more expensive and lacks standardized protocols.
- The review highlights the current state of MPS application for detecting short tandem repeats (STRs) in forensic genetics, addresses urgent challenges, and discusses future possibilities for its integration into forensic practices.
Objective: To improve the method of sorting undifferentiated and differentiated spermatogonial cells by magnetic bead sorting with specific antibodies.
Methods: Using the magnetic bead sorting technique combined with Thy1 and c-Kit specific antibodies, we sorted Thy1+ and c-Kit+ cells in the testis of 7-postnatal-day male mice as undifferentiated and differentiated spermatogonia, respectively. We determined the purities of the two types of spermatogonial cells by immunofluorescence and flow cytometry, identified them via the differential expressions of Gfrα1, Plzf, c-Kit and Sohlh2 by real-time quantitative PCR, and cultured the Thy1+ cells primarily.
This study aims to investigate the therapeutic effects of papain elastic liposomes (PEL) on hypertrophic scar through topical application. PEL were prepared using the reverse-phase evaporation method and optimized by response surface methodology. The transdermal absorption of optimized PEL was tested by vertical Franz diffusion cells in vitro.
View Article and Find Full Text PDFObjective: To explore the correlation between postmortem interval (PMI) and five RNA markers of rat's skin--β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA(18S rRNA), 5S ribosomal RNA (5S rRNA), and microRNA-203 (miR-203), at different temperatures.
Methods: Eighteen SD rats were randomly divided into three environmental temperature groups: 4 °C, 15 °C and 35 °C, respectively. Skin samples were taken at 11 time points from 0 h to 120 h post-mortem.
Objective: To observe the changes of relative expression of myocardial various RNAs in rats died of different causes and their relationship with PMI.
Methods: The rat models were established in which the rats were sacrificed by broken neck, asphyxia, and hemorrhagic shock. Total RNAs were extracted from myocardium.
[Application of molecular autopsy in sudden death caused by inherited arrhythmia].
Fa Yi Xue Za Zhi
October 2012
Article Synopsis
- * Inherited arrhythmias can cause lethal heart rhythms leading to SCD, but identifying their cause can be tricky during autopsies since there are usually no obvious heart abnormalities.
- * Recent research highlights the importance of detecting genetic mutations related to these inherited arrhythmias using molecular biology techniques, especially in cases where traditional autopsy findings are inconclusive.