Clinical application of 3D slicer reconstruction and 3D printing localization combined with neuroendoscopy technology in VPS surgery.

To explore techniques, advantages and disadvantages of 3D Slicer reconstruction and 3D printing localization technology combined with transcranial neuroendoscopy in ventriculoperitoneal shunt surgery. Retrospective analysis of clinical data of patients with hydrocephalus treated by ventriculoperitoneal shunt surgery using 3D Slicer reconstruction and 3D printing positioning technology combined with transcranial neuroendoscopy in our hospital from October 2021 to March 2023. A total of 33 patients with complete data were collected, including 19 males and 14 females, aged 10-81 years.

Prevalence of excessive daytime sleepiness (EDS) and its association with quality of life in patients with obstructive sleep apnea (OSA): data from a sleep-center in Shenzhen, a single-center cross-sectional study.

Background: Excessive daytime sleepiness (EDS) is considered to be one of the main clinical manifestations of obstructive sleep apnea (OSA) and is a treatment target for patients with OSA. The prevalence of EDS in patients with OSA remains unclear and there is a lack of studies on the associations of EDS with quality of life among patients with OSA in China. This study aimed to evaluate the prevalence of EDS and its association with quality of life in patients with OSA in Shenzhen, China.

Developmental changes of skin quality from breast, back, and thigh of Pekin ducks from 1 to 6 weeks of age.

Good skin quality not only improved carcass quality but also increased consumer demand for fresh poultry meat. This study aimed to investigate the developmental changes in skin growth and quality of Pekin ducks during 1-6 weeks of age. The skin samples were collected from the breast, back, and thigh tissues of six male ducks at the end of each week.

Genetic code expansion reveals site-specific lactylation in living cells reshapes protein functions.

Protein lactylation is an emerging field. To advance the exploration of its biological functions, here we develop a comprehensive workflow that integrates proteomics to identify lactylated sites, genetic code expansion (GCE) for the expression of site-specifically lactylated proteins in living cells, and an integrated functional analysis (IFA) platform to evaluate their biological effects. Using a combined wet-and-dry-lab proteomics strategy, we identify a conserved lactylation at ALDOA-K147, which we hypothesize plays a significant biological role.

Senescent retinal pigment epithelial cells promote angiogenesis in choroidal neovascularization via the TAK1/p38 MAPK pathway.

Senescent retinal pigment epithelial cells play a key role in neovascular age-related macular degeneration (nAMD); however, the mechanisms underlying the angiogenic ability of these cells remain unclear. Herein, we investigated the effects of the senescent adult retinal pigment epithelial cell line-19 (ARPE-19) on wound healing, cell migration and survival, and tube formation abilities of human umbilical vein endothelial cells (HUVECs). Additionally, we used Brown Norway rats to establish a laser-induced choroidal neovascularization (CNV) model for further nAMD-related studies.

Real-world evidence on the efficacy and safety of vonoprazan-amoxicillin dual therapy for treatment in elderly patients.

Background: A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of (). While substantial research supports the efficacy and safety of vonoprazan and amoxicillin (VA) dual therapy in the general population, there is still a lack of studies specifically focusing on its safety in elderly patients.

Aim: To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for in elderly patients.

Strategy for Fabricating Degradable Low-Surface-Energy Cross-Linked Networks with Excellent Anti-Fouling Properties.

Marine biofouling negatively impacts marine industries and ship navigation. However, current coatings are based on a single antifouling mechanism, which is insufficient to cope with the complex and ever-changing marine environment. Herein, multifunctional antifouling coatings were developed using a material system containing perfluoropolyether and caprolactone chains.

Supplemental glucose oxidase as an antibiotic substitute alleviates diarrhea and improves intestinal health in weaned piglets.

Weaning stress-induced diarrhea is a serious issue in pig production. This study aimed to evaluate the potential of glucose oxidase (GOD) as an antibiotic substitute to alleviate diarrhea and improve gut health in weaned piglets. According to a randomized complete block design, 250 piglets weaned around 21 d of age were allocated into 5 groups (5 replicates/group), which received a basal diet without or with supplemental 200 mg/kg antibiotic, 500, 1000 and 2000 U/kg GOD, respectively.

The expression of CCL17 and potential prognostic value on tumor immunity in thyroid carcinoma based on bioinformatics analysis.

Although CCL17 has been reported to exert a vital role in many cancers, the related studies in the thyroid carcinoma have never reported. As a chemokine, CCL17 plays a positive role by promoting the infiltration of immune cells into the tumor microenviroment (TME) to influence tumor invasion and metastasis. Therefore, this study is aimed to investigate the association of CCL17 level with potential prognostic value on tumor immunity in the thyroid carcinoma (THCA) based on the bioinformatics analysis.

Design of upper limb muscle strength assessment system based on surface electromyography signals and joint motion.

Purpose: This study aims to develop a assessment system for evaluating shoulder joint muscle strength in patients with varying degrees of upper limb injuries post-stroke, using surface electromyographic (sEMG) signals and joint motion data.

Methods: The assessment system includes modules for acquiring muscle electromyography (EMG) signals and joint motion data. The EMG signals from the anterior, middle, and posterior deltoid muscles were collected, filtered, and denoised to extract time-domain features.

Expanding horizons: genetic code expansion technology in the study of PTM functions.

Recent advancements in Genetic Code Expansion (GCE) have significantly enhanced our understanding of post-translational modifications (PTMs), which are critical for protein regulation. GCE facilitates the precise incorporation of unnatural amino acids (UAAs) at specific sites within proteins of interest (POIs), making it a powerful tool for modulating PTMs in vivo. This review summarizes the various UAAs utilized to directly incorporate PTMs into proteins through GCE, with a focus on their applications in both histone and non-histone PTMs research.

Aloe-emodin plus TIENAM ameliorate cecal ligation and puncture-induced sepsis in mice by attenuating inflammation and modulating microbiota.

Despite the high sepsis-associated mortality, effective and specific treatments remain limited. Using conventional antibiotics as TIENAM (imipenem and cilastatin sodium for injection, TIE) is challenging due to increasing bacterial resistance, diminishing their efficacy and leading to adverse effects. We previously found that aloe-emodin (AE) exerts therapeutic effects on sepsis by reducing systemic inflammation and regulating the gut microbiota.

Fractional Carbon Dioxide Laser Combined with Secukinumab in the Treatment of Refractory Psoriasis Lesions on the Lower Legs.

Psoriasis is a chronic autoimmune skin disorder characterized by high morbidity and a tendency for recurrence. Although biologic therapy for lesion clearance can achieve PASI 90/100, refractory sites remain challenging to completely clear, particularly on the lower legs and elbows. To explore the efficacy of novel treatments for these resistant sites, we present a clinical observation on the safety and effectiveness of fractional carbon dioxide (CO2) laser therapy combined with secukinumab for four patients with moderate-to-severe plaque psoriasis.

Crosstalk between gut microbiota and cancer chemotherapy: current status and trends.

Background: Chemotherapy is crucial in the management of tumors, but challenges such as chemoresistance and adverse reactions frequently lead to therapeutic delays or even premature cessation. A growing body of research underscores a profound connection between the gut microbiota (GM) and cancer chemotherapy (CC). This paper aims to pinpoint highly influential publications and monitor the current landscape and evolving trends within the realm of GM/CC research.

Combined Abrocitinib and Acitretin Therapy for Darier's Disease: A Case Report.

Darier's disease (DD) is a rare chronic keratinizing skin disease characterized by dyskeratosis of epidermal cells. We report a case of DD with a medical history spanning over 20 years and recurring symptoms. Pathologically confirmed DD was treated with a combination of abrocitinib and acitretin, resulting in rapid symptom resolution within 2 weeks.

Evaluating the diagnostic performance of [F]ALF-NOTA-FAPI-04 PET/CT in gastric cancer: a comparative study with [F]FDG PET/CT.

Purpose: To compare the diagnostic value of [F]ALF-NOTA-FAPI-04 positron emission tomography/computed tomography (PET/CT) and F-fluorodeoxyglucose (FDG) PET/CT in gastric cancer.

Methods: This single-center retrospective analysis included 65 patients with gastric cancer who received both [F]FDG and [F]ALF-NOTA-FAPI-04 PET/CT for initial staging or restaging. Histopathological manifestations, typical imaging manifestations, follow-up imaging, and comprehensive clinical assessment were used as reference criteria.

Impact of fat intake on [F]AlF-NOTA-FAPI-04 uptake in normal abdominal organs.

Purpose: [F]AlF-NOTA-FAPI-04 demonstrates significant physiological uptake in the gallbladder and biliary tract system, representing a limitation of this positron emission tomography (PET) tracer. The aim of this study was to evaluate the impact of milk consumed prior to a PET/CT scan on [F]AlF-NOTA-FAPI-04 uptake in normal abdominal organs.

Materials And Methods: A total of 86 patients who underwent [F]AlF-NOTA-FAPI-04 PET/CT imaging took part in this single-center retrospective clinical study at the Hunan Cancer Hospital between December 2020 and August 2021.

Subcellular Proteomic Mapping of Lysine Lactylation.

Protein lactylation is a novel post-translational modification (PTM) involved in many important physiological processes such as macrophage polarization, immune regulation, and tumor cell growth. However, traditional methodologies for studying lactylation have predominantly relied on peptide enrichment from whole-cell lysates, which tend to favor the detection of high-abundance peptides, thus limiting the identification of low-abundance lactylated peptides. To address this limitation, here, we employed subcellular fractionation to separate proteins and map lactylated peptides from each isolated subcellular fraction using a model cell line.

Effects of cricket powder on structural and mechanical properties of soy protein isolate extrudates.

This study investigated the impact of cricket powder (CP) incorporation on the structural and mechanical properties of soy protein isolate (SPI) extrudates. The physicochemical properties of CP, rheological properties of SPI-CP blends and their potential structuring properties were evaluated. The results showed that CP had a high protein content (72.

The Protective Effects of and Tenuifolin on Corticosterone-Evoked Ferroptosis, Oxidative Stress, and Neuroinflammation: Insights from Molecular Dynamics Simulations and In Vitro Experiments.

Excessive stress is a well-established contributor to neurological damage, insomnia, and depression, imposing a significant burden on individuals and society. This underscores the urgent need for effective stress-relief strategies. The main purpose of this study was to explore the protective effects of (PT) and its bioactive compound, tenuifolin, against corticosterone-induced neurotoxicity, with a focus on ferroptosis, oxidative stress, and neuroinflammation.

Host-derived alleviates hyperuricemia by improving gut microbial community and hydrolase-mediated degradation of purine nucleosides.

The gut microbiota is implicated in the pathogenesis of hyperuricemia (HUA) and gout. However, it remains unclear whether probiotics residing in the host gut, such as , can prevent HUA development. Herein, we isolated SQ001 from the cecum of HUA geese and conducted in vitro assays on uric acid (UA) and nucleoside co-culture.

Leech granules inhibit ferroptosis and alleviate renal injury in mice with diabetic kidney disease.

Ethnopharmacological Relevance: The underlying mechanisms of diabetic kidney disease (DKD) and effective treatment strategies remain unclear. DKD progression is closely associated with abnormal iron metabolism and ferroptosis in vivo. Leeches, used in traditional Chinese medicine for promoting blood circulation and resolving blood stasis, are utilized to treat diabetes and its associated complications.