Publications by authors named "Yaying Wu"

Lithocholic acid (LCA) is accumulated in mammals during calorie restriction and it can activate AMP-activated protein kinase (AMPK) to slow down ageing. However, the molecular details of how LCA activates AMPK and induces these biological effects are unclear. Here we show that LCA enhances the activity of sirtuins to deacetylate and subsequently inhibit vacuolar H-ATPase (v-ATPase), which leads to AMPK activation through the lysosomal glucose-sensing pathway.

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Purpose: To evaluate the clinical characteristics and viral Colonization of corneas donated by volunteers with coronavirus disease 2019 (COVID-19) before and after corneal transplantation.

Methods: We retrospectively compared the characteristics and clinical outcomes of patients who received corneas from donors with and without a history of COVID-19 after corneal transplantation. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to evaluate the expression of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA on ocular surfaces in corneal preservation solutions as well as the recipients' tears.

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The shift of carbon utilization from primarily glucose to other nutrients is a fundamental metabolic adaptation to cope with decreased blood glucose levels and the consequent decline in glucose oxidation. AMP-activated protein kinase (AMPK) plays crucial roles in this metabolic adaptation. However, the underlying mechanism is not fully understood.

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Article Synopsis
  • - Glycolytic metabolites, like 3-phosphoglycerate (3-PGA), are not just for energy but can regulate cell fate by influencing p53 activity related to apoptosis.
  • - Low 3-PGA levels shift phosphoglycerate dehydrogenase (PHGDH) function from serine synthesis to activating p53, promoting apoptosis through interactions with proteins like AXIN and HIPK2.
  • - PHGDH mutations can either hinder or enhance p53 activation and apoptosis in liver cancer cells, with caloric restriction providing a potential method to control cancer growth by lowering glucose levels and affecting PHGDH activity.
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Tear film hyperosmolarity plays a core role in the development of dry eye disease (DED) by mediating the disruption of ocular surface homeostasis and triggering inflammation in ocular surface epithelium. In this study, the mechanisms involving the hyperosmolar microenvironment, glycolysis mediating metabolic reprogramming, and pyroptosis were explored clinically, in vitro, and in vivo. Data from DED clinical samples indicated that the expression of glycolysis and pyroptosis-related genes, including PKM2 and GSDMD, was significantly upregulated and that the secretion of IL-1β significantly increased.

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Two new species of the genus Pseudopoda Jäger, 2000 are reported from Yunnan Province, China: Pseudopoda mingshengi sp. nov. (male, female) and P.

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Variants in the may cause antenatal transient Bartter syndrome, which is characterised by polyhydramnios, preterm labour, postnatal polyuria, hypokalaemia and metabolic alkalosis. Transient gross hematuria and acute kidney injury in such cases have not been reported previously. The patient, a boy, was born at a gestational age of 27 + 5 weeks.

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Background: The family Macrothelidae Simon, 1892 belongs to the infraorder Mygalomorphae, currently contains two genera and 47 described species, from South Europe, South, and East Southeast Asia, Central, West, and North Africa.

New Information: Two new species of the funnel-web spider genus Ausserer, 1871 from Yunnan Province, China are described: Wu & Yang, sp. n.

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  • * The study reveals that blocking the sorting mechanism that delivers EGFR to the plasma membrane can be an effective approach for treating EGFR-dependent tumors, with palmitoylation playing a crucial role in this process.
  • * A new cell-permeable peptide, N-myristoylated GKVL-TAT, has been developed to disrupt EGFR's plasma membrane localization, demonstrating significant inhibition of tumor progression, thereby indicating its therapeutic potential.
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  • The study reveals that low glucose levels activate a specific version of AMPK, a protein that helps regulate energy metabolism, through the enzyme aldolase, which isn't bound to fructose-1,6-bisphosphate (FBP).
  • Researchers identified a small molecule called aldometanib that blocks FBP from binding to aldolase, leading to the activation of lysosomal AMPK and inducing beneficial metabolic effects in rodents.
  • Aldometanib demonstrated the ability to lower glucose levels without causing hypoglycemia, improve conditions like fatty liver disease, and even extend lifespan and healthspan in laboratory models, suggesting its potential as a treatment for metabolic disorders in humans.
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Purpose: To investigate the effects of high-intensity use of smartphones on ocular surface homeostasis and to explore whether high-intensity use of handheld digital devices can cause false increase of dry eye diagnostic rate.

Methods: In this prospective self-control study, 60 subjects (120 eyes) were recruited and asked to read on smartphones provided by the same manufacturer for two consecutive hours. This study was conducted during 8:00 - 10:00 AM to eliminate the influence of digital equipment used the previous day.

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This study aims to investigate the reliability and efficacy of rear 4-min Schirmer test, as a supplement indicator, in assessing tear secretion and diagnosing dry eye. 180 participants were enrolled in this study. Schirmer test I without anaesthesia was performed once on both eyes to determine the value of normal Schirmer test.

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Abundant ion-channels, including various perceptual receptors, chloride channels, purinergic receptor channels, and water channels that exist on the ocular surface, play an important role in the pathogenesis of dry eye. Channel-targeting activators or inhibitor compounds, which have shown positive effects in and experiments, have become the focus of the dry eye drug research and development, and individual compounds have been applied in clinical experimental treatment. This review summarized various types of ion-channels on the ocular surface related to dry eye, their basic functions, and spatial distribution, and discussed basic and clinical research results of various channel receptor regulatory compounds.

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Purpose: To investigate the efficacy of intense pulsed light (IPL) combined with deproteinized calf blood extract (DCBE) eye drops for dry eye disease (DED) patients with nociceptive ocular pain.

Methods: In this prospective, one-center, interventional study, 23 subjects with DED and ocular pain were treated with a combination of IPL and DCBE eye drops for four sessions at a four-week interval. Subjective and objective assessments on nociceptive pain and dry eye were examined and analyzed.

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Purpose: Investigate the correlation and agreement between the results of semiautomated and fully automated quantitative analysis of the corneal sub-basal nerve plexus (SNP) in patients with dry eye disease (DED) with ocular pain using confocal microscopy (IVCM).

Method: A total of 50 voluntary participants were enrolled in this study, i.e.

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Article Synopsis
  • Metformin, widely used for diabetes treatment, also shows potential benefits in anti-aging and cancer prevention, primarily activating AMPK through an unknown mechanism.
  • Recent research identifies that metformin inhibits the v-ATPase lysosomal proton pump, which is crucial for AMPK activation, by binding to a protein called PEN2.
  • Knockout experiments demonstrate that PEN2 is essential for metformin's effects on reducing liver fat and lowering blood glucose, and it also plays a role in extending lifespan in the model organism Caenorhabditis elegans.
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Dry eye-related ocular surface examination is very important in the diagnosis and treatment of dry eye disease. With the recent advances in science and technology, dry eye examination techniques have progressed rapidly, which has greatly improved dry eye diagnoses and treatment. However, clinically, confusion remains about which examination to choose, how to ensure the repeatability of the examination, and how to accurately interpret the examination results.

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  • Targeted analysis of data-independent acquisition (DIA) data requires a spectral library, typically generated from data-dependent acquisition (DDA) experiments or directly from DIA data.
  • The study compares various spectral libraries to assess their impact on analyzing diaPASEF data, revealing differences in proteome coverage and data quality.
  • Although the library-free method identifies fewer proteins than the library-based approach, it maintains a high level of data completeness and yields similar biological insights.
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This paper aims to investigate the efficacy of circularly polarized light smartphones in affecting dry eye symptoms and asthenopia through a comparison with linearly polarized smartphones. One hundred twenty participants were randomly divided into four groups. Dry eye and asthenopia symptoms were evaluated using the Ocular Surface Disease Index (OSDI), Computer Vision Syndrome Scale 17 (CVSS17), Convergence Insufficiency Symptom Survey (CISS), and visual analogue scale (VAS).

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Objectives: To explore the role of the corneal sensory nerves in Pseudomonas aeruginosa (P. aeruginosa) keratitis, the synergistic effect between the sensory neurons and macrophages in calcitonin gene-related peptide (CGRP) release, and the functional mechanisms of CGRP-mediated transformation of macrophages to the M2 phenotype.

Methods: Corneal nerve loss, macrophage recruitment, and CGRP expression were evaluated.

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This study aims to investigate the reliability and efficacy of maximum fluorescein tear break-up time (FTBUTmax) in diagnosing dry eye disease (DED). 147 participants were enrolled in this study. Ocular symptoms were assessed by Ocular Surface Disease Index (OSDI).

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Introduction: Pterygium is a common chronic ocular surface condition in ophthalmology.At present, the main treatment modality is surgical resection. Although the recurrence rate can be controlled to varying degrees, some patients can still develop serious complications, such as scleral melting, corneal melting, and even corneal perforation.

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Background: To evaluate the therapeutic effect of intense pulsed light (IPL) therapy with optimal pulse technology (OPT) on meibomian gland dysfunction (MGD) with and without ocular infestation.

Methods: This prospective study included 150 patients with MGD who were divided into -infested (DI) and non-infested (control) groups according to counts determined by epilating eyelashes to evaluate. Each patient underwent three OPT treatment sessions at 3-week intervals.

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